A5087906581- Wound Healing and Treatments
- Mesenchymal stem cell research
- Extracellular vesicles in disease
- Craniofacial Disorders and Treatments
- Cleft Lip and Palate Research
- Hedgehog Signaling Pathway Studies
- Spine and Intervertebral Disc Pathology
- Surgical site infection prevention
- Orthopedic Infections and Treatments
- Reconstructive Surgery and Microvascular Techniques
- Dermatologic Treatments and Research
- Medical and Biological Sciences
- Bone Metabolism and Diseases
- Salivary Gland Disorders and Functions
- Reconstructive Facial Surgery Techniques
- Cancer and Skin Lesions
- Bone fractures and treatments
- Fibroblast Growth Factor Research
- Salivary Gland Tumors Diagnosis and Treatment
- Hair Growth and Disorders
- Skin Protection and Aging
- Proteoglycans and glycosaminoglycans research
- Dental Trauma and Treatments
- Angiogenesis and VEGF in Cancer
- Cardiovascular Disease and Adiposity
Peking University
2020-2024
Peking University Third Hospital
2020-2024
The potential of relapse craniofacial disharmony after trans-sutural distraction osteogenesis is high due to the failure produce a stable bone bridge in suture gap. aim this study evaluate whether hydroxyapatite nanoparticles (nHAP) have effect promoting osteoblast differentiation suture-derived stem cells (SuSCs) and formation sagittal during expansion.SuSCs were isolated from sutures exposed various concentrations nHAP (0, 25, 50, 100 μg mL-1) determine optimal concentration via performing...
Abstract To investigate whether human umbilical cord mesenchymal stem cell‐derived exosomes combined with gelatin methacryloyl (GelMA) hydrogel are beneficial in promoting healing of laser‐injured skin wounds mice. Supernatants cultured cells (HUC‐MSCs) were collected to obtain MSC‐derived (HUC‐MSCs‐Exos), which GelMA complex treat a mouse fractional laser injury model. The study was divided into PBS group, EX (HUC‐MSCs‐Exos) GEL (GelMA hydrogel) group and EX+GEL (HUC‐MSCs‐Exos group. each...
Trans-sutural Distraction Osteogenesis (TSDO) is a common surgical method for treating facial hypoplasia in secondary cleft lip and palate. Its mechanism involves utilizing mechanical traction to induce the differentiation of mesenchymal stem cells（MSCs） within into osteoblasts. Retinoic acid (RA) involved various life processes promotes osteogenic under low-dose conditions. However, impact RA treatment on cells force unclear. To investigate this mechanism, we performed cell stretching...
Three-dimensional cell-laden tissue engineering has become an extensive research direction. This study aimed to evaluate whether chondrocyte spheroids (chondro-spheroids) prepared using the hanging-drop method could develop better cell proliferation and morphology maintenance characteristics, be optimized as a micro unit for cartilage engineering. Chondro-spheroids were loaded into cross-linkable hybrid hydrogel of gelatin methacrylate (GelMA) hyaluronic acid (HAMA) in vivo vitro. Cell...
To investigate the clinical application value of different flap transfer and repair techniques in adult patients with chronic osteomyelitis limbs complicated soft tissue defects. According to characteristics defects 21 cases, plastic surgery was applied, including debridement, negative pressure device, cover wound. Among cases local defect, 15 were repaired sural neurotrophic musculocutaneous transfer, 2 medial plantar skin ilioinguinal 1 patient z-forming wound, soleus muscle combined...
Extracellular vesicles (EVs) are specific subcellular released by cells under various environmental conditions. Tumescent liposuction is a commonly used procedure in plastic surgery practice. In the present study, we aimed to extract EVs derived from lipoaspirate fluid (LF-EVs) and characterize them using transmission electron microscopy, nanoparticle tracking analysis, western blotting. The global profiles of proteins microRNAs LF-EVs were identified, strongly suggesting potential...
Wound healing undergoes intricate phases: hemostasis, inflammation, proliferation, and remodeling. Stem cell therapy based on adipose tissue-derived stem exosomes (ADSCs-exo) is considered a potential effective treatment for accelerating wound healing. However, the molecular mechanisms of using ADSCs-exo remain largely unknown.Circular wounds, 1 × cm, were generated C57BL/6 mice, followed by OriCell mouse adipose-derived mesenchymal suspension treatment, area was measured recorded at days 0,...
Abstract To investigate the role of GLI1 on skin proliferation and neovascularization during expansion in mice. We constructed GLI1‐cre/R26‐Tdtomato GLI1‐cre/R26‐mtmg gene‐tagged mouse models. Using a two‐photon vivo imaging instrument to observe changes number distribution GLI1(+) cells process clarify spatial relationship between blood vessels process. In vitro assays were performed further validate effects SHH (sonic hedgehog) its downstream component cell viability. Finally, qRT‐PCR was...
Injuries and tumours of the cervical spine represent therapeutic challenges to treating surgeon due complex anatomical relationships biomechanical features. The anterior midline (ACM) retropharyngeal (ACR) approaches are effective safe surgical for certain lesions, such as neoplasms, atlantoaxial subluxation, odontoid fractures. Posterior pharyngeal wall defects (PPWDs) is one most frequently encountered morbidities after surgery (ACSS). However, limited information has been published...
Abstract This study aimed to explore the treatment effect of Z‐plasty on a non‐healing wound. A total 72 patients diagnosed with chronic wound in Peking University Third Hospital from November 2009 August 2019 were retrospectively analysed. Among them, 27 treated Z‐plasty, and 45 general method. Detailed patient information was retrieved medical records, including age, gender, body mass index (BMI), alcohol, smoking, comorbidities (diabetes mellitus, hypertension, heart disease). Surgical...
Abstract Background Trans-sutural distraction osteogenesis (TSDO) involves the application of force to facial sutures stimulate osteogenesis. Gli1 + cells in cranial play an important role bone growth. However, whether differentiate into under is unknown. Methods 4-week-old Gli1ER/Td and C57BL/6 mice were used establish a TSDO model explore zygomaticomaxillary sutures. A cell lineage tracing was observe distribution remodeling. Results Distraction promoted remodeling during TSDO....
Minor salivary gland mesenchymal stem cells (MSGMSCs) can be easily extracted and have a broad range of sources. Applying exosomes to wounds is highly promising method for promoting wound healing. Exosomes derived from different cell types been proven enhance healing, with adipose-derived (ADSC)-derived being the most extensively researched. Considering that MSGMSCs advantages such as easier extraction compared ADSCs, should also very type in exosome therapy. However, whether MSGMSC-derived...
Dedifferentiated adipose cells (DAs) and adipose‑derived stem (ADSCs) are two of the primary types derived from tissue, which have been reported to possess similar characteristics, but also exhibit unique phenotypic functional advantages. However, several reports described inconsistent results regarding their differences in multilineage differentiation function. Moreover, best our knowledge, there no studies assessing myogenic ability, or transcriptome between cell lipoaspirates via...
We aimed to explore the clinical significance of secondary pedicle amputation repair distal defects with pedicled axial flap. Five patients who underwent flap transfer a large area skin and soft tissue in our hospital were included this retrospective study. Detailed information including general data data, such as preoperative complication, type primary wound, distance between wound donor site (cm), postoperative complications, types collected. The had good joint movement at 6 months after...