The potential of relapse craniofacial disharmony after trans-sutural distraction osteogenesis is high due to the failure produce a stable bone bridge in suture gap. aim this study evaluate whether hydroxyapatite nanoparticles (nHAP) have effect promoting osteoblast differentiation suture-derived stem cells (SuSCs) and formation sagittal during expansion.SuSCs were isolated from sutures exposed various concentrations nHAP (0, 25, 50, 100 μg mL-1) determine optimal concentration via performing...

Trans-sutural Distraction Osteogenesis (TSDO) is a common surgical method for treating facial hypoplasia in secondary cleft lip and palate. Its mechanism involves utilizing mechanical traction to induce the differentiation of mesenchymal stem cells（MSCs） within into osteoblasts. Retinoic acid (RA) involved various life processes promotes osteogenic under low-dose conditions. However, impact RA treatment on cells force unclear. To investigate this mechanism, we performed cell stretching...

Although maxillary distraction osteogenesis has been used for early treatment of midfacial hypoplasia, the inevitable osteotomies are still a complicated and invasive procedure growing patients. Based on bone-borne trans-sutural osteogenesis, novel improvements to approach were made treat clinical outcomes skeletal changes analyzed.Seventy consecutive cleft lip palate patients with hypoplasia treated osteogenesis. The system consists rigid external distractor, nickel-titanium shape memory...

Objective The aim was to evaluate clinical application of autologous fat transplantation in the posterior pharynx treat velopharyngeal incompetence and cleft palate. Study Design Case series with chart review. Setting Cleft Lip Palate Center Plastic Surgery Hospital, an academic medical center. Subjects Methods We studied 11 patients (age, 5‐26 years) a palate insufficiency who underwent injection. Patients were followed for 9 40 months. Pronunciation evaluation, visual appearance...

In Brief Background microform cleft lip repair, reconstructing the elaborate structures is difficult. We describe a new technique of unilateral repair that based on muscle tension line group theory. Methods According to shape Cupid bow, different small incision used without creating an obvious cutaneous scar. First, nasolabial around nasal floor (the first auxiliary group) reconstructed, and then orbicularis oris philtrum second reconstructed Results From June 2006 2012, was in 263 repairs....

This retrospective study describes various buccinator musculomucosal flaps for the repair of palatal fistulae. Twenty-two fistulae were repaired at our institution between 2002 and 2012 by with superior-anterior or posterior pedicles. Seventeen patients treated posteriorly pedicled flaps, 7 whom had lengthening soft palate 10 simultaneously severe lateral scarring. Five anterior midpalatal superior-anteriorly flaps. All but 4 22 satisfactory results. Four recurrent fistulae, 2 resulting from...

Midface distraction osteogenesis has been popularized for the correction of midface hypoplasia associated with exophthalmos and obstructive sleep apnea in patients Crouzon syndrome. The purpose this study was to present method utilizing modified external device elastic advancement syndrome, clinical outcomes skeletal changes were analyzed.Five consecutive syndrome underwent Le Fort III osteotomy using a distraction. system consists rigid distractor, nickel-titanium shape memory alloy spring,...

Bilateral cleft lip is considered to be the most difficult repair. More and more surgeries are concerned about reconstruction of orbicularis muscle pursuit philtrum shaping in bilateral repair, but have little success. Years study on children with resulted authors' creation tension line groups theory. Through implementation this theory, authors achieved favorable results microform The also applied concept repair.Between January 2010 December 2014, 31 received operations department, underwent...

Rigid external distraction is currently performed to correct cases of severe maxillary hypoplasia. As an improvement this technique, we propose the use intranasal bone-borne traction hook. This study a retrospective chart review hooks used in treatment There were 110 patients treated with from 2005 2017. The maximum force was 7.75 kg, and there few complications encountered during distraction. 76 who had removed under local anaesthesia. A cephalometric analysis conducted 56 patients. average...

Abstract Trans-sutural distraction osteogenesis (TSDO) promotes midface growth in growing cleft lip and palate (CLP) patients with midfacial hypoplasia. The superficial skeletal changes after therapy revealed rotation advancement of the skeleton associated differential displacement each segment, but reports rarely focus on internal structures, including circummaxillary sutures, maxillary tuberosity sinus, which may play a crucial role during this process. This study evaluated computed...

Abstract To investigate the role of GLI1 on skin proliferation and neovascularization during expansion in mice. We constructed GLI1‐cre/R26‐Tdtomato GLI1‐cre/R26‐mtmg gene‐tagged mouse models. Using a two‐photon vivo imaging instrument to observe changes number distribution GLI1(+) cells process clarify spatial relationship between blood vessels process. In vitro assays were performed further validate effects SHH (sonic hedgehog) its downstream component cell viability. Finally, qRT‐PCR was...

Midfacial hypoplasia is a common maxillofacial deformity in patients with cleft lip and palate, which requires surgical treatment. However, trans-sutural distraction osteogenesis (TSDO) remains some disadvantages, including difficulty accurate location of path, prolonged operation time, excess bleeding. This study aimed to evaluate the application an optical navigation system (OSNS) developed for TSDO. Six consecutive midfacial who required TSDO were included study. Preoperatively, head...

The purposes of this study were to evaluate the effects transsutural distraction osteogenesis applied maxillary complex with a new internalized device and analyze long-term outcome.Three-month-old beagle dogs treated self-designed distractor. feasibility was evaluated, growth measured using radiography computed tomography (CT). regenerated bone examined micro-CT, biomechanical testing, histology 1 year after distraction.The experimental group showed significantly larger forward displacement...

Trans-sutural distraction osteogenesis (TSDO) has been successfully used to correct midfacial hypoplasia in growing patients for years. The effects of TSDO, however, remain difficult predict adult patients. aim this study was determine the biologic basis age-related increase difficulty performing TSDO. A total 45 male Sprague Dawley (SD) rats were obtained 3 age groups: 4 weeks old (4W, N = 15), months (3M, and 13 (13M, 15). zygomaticomaxillary sutures (ZMS) dissected, their morphology...

Skeletal muscle regeneration mainly depends on satellite cells; however, these cells are not sufficient for supporting repair and in volumetric loss (VML), Duchenne muscular dystrophy, other injuries or diseases. As such, much work has been conducted recent years to search myogenic stem cells. Adipose-derived (ADSCs) have a wide range of sources, rapid growth, multi-directional differentiation potential, become vital candidates regeneration. Multiple factors influence the capacity ADSCs....

To propose landmarks and a new coordinate system to aid three-dimensional cephalometric analysis of adolescent cleft lip palate (CLP) using computed tomography (CT) imaging.Sixty-four-row CT images obtained from 52 patients were retrospectively analyzed with the MIMICS program (MIMICS 10.02; Materialise Technologies, Leuven, Belgium) determine intrarater reliability for before surgery.Five points located on each image including midpoint between both uppermost external auditory meatus (EAM),...