A5018024499- Cancer-related molecular mechanisms research
- MicroRNA in disease regulation
- Hepatocellular Carcinoma Treatment and Prognosis
- Extracellular vesicles in disease
- RNA modifications and cancer
- Ferroptosis and cancer prognosis
- Circular RNAs in diseases
- Pancreatic and Hepatic Oncology Research
- Cancer, Hypoxia, and Metabolism
- Cancer-related gene regulation
- Cancer, Lipids, and Metabolism
- Epigenetics and DNA Methylation
- RNA Research and Splicing
- Immune Cell Function and Interaction
- Inflammatory Biomarkers in Disease Prognosis
- Molecular Biology Techniques and Applications
- Immune cells in cancer
- Mesenchymal stem cell research
- Angiogenesis and VEGF in Cancer
- Cancer Cells and Metastasis
- Cancer Genomics and Diagnostics
- IL-33, ST2, and ILC Pathways
- Genetic factors in colorectal cancer
Ajou University
2020-2025
Catholic University of Korea
2021
Samsung Medical Center
2006
Sungkyunkwan University
2006
Abstract Background Cancer‐associated fibroblasts (CAFs) play an important role in the induction of chemo‐resistance. This study aimed to clarify mechanism underlying CAF‐mediated resistance two tyrosine kinase inhibitors (TKIs), sorafenib and lenvatinib, identify a novel therapeutic target for overcoming TKI hepatocellular carcinoma (HCC). Methods We performed systematic integrative analysis publicly available gene expression datasets whole‐transcriptome sequencing data from 9 pairs CAFs...
Exosomal microRNAs (exo-miRs) have been promising cancer biomarkers. MiRs in hepatocellular carcinoma (HCC) cell-derived exosomes (HEX) were analyzed to identify reliable serum biomarkers for HCC. To detect overexpressed miRs HEX, extracted exosomal small RNAs from human HCC cell lines and normal hepatocytes sequenced analyzed. Clinical significance of the HEX was evaluated using quantitative real-time PCR (qRT-PCR) on samples a validation cohort consisting 28 healthy individuals, 60 with...
This study aimed to identify novel long noncoding RNA (lncRNA) biomarkers for hepatocellular carcinoma (HCC) using publicly available tissue genomic datasets and validate their diagnostic utility early‐stage HCC. Differentially expressed lncRNAs between 371 HCC 50 nontumor tissues were obtained from The Cancer Genome Atlas liver (TCGA_LIHC) project. Subsequently, the expression of serum‐ extracellular vesicle (EV)‐derived lncRNA was assessed in 10 patients with healthy controls RT–qPCR....
Abstract Currently, a reliable serum biomarker for hepatocellular carcinoma (HCC) has not been established, particularly early‐stage HCC (single tumor < 2 cm). We aimed to investigate diagnostic exosomal microRNA (exo‐miR) panel HCC. Driver oncogenic miR (onco‐miR) candidates were selected by integrative analysis of expression profiles from three different RNA sequencing datasets human Expressions onco‐miRs in exosome measured using quantitative real‐time PCR. Diagnostic performances...
Hepatocellular carcinoma (HCC) is characterized by high recurrence and mortality, necessitating the identification of reliable biomarkers. In this study, we aimed to identify predictive gene signatures for HCC evaluate efficiency GULP PTB domain-containing engulfment adaptor 1 (GULP1) as a diagnostic marker therapeutic target HCC. We analyzed genomic datasets from The Cancer Genome Atlas Gene Expression Omnibus databases via least absolute shrinkage selection operator Cox regression 10-fold...
This study investigated the diagnostic potential of serum small extracellular vesicle-derived long noncoding RNAs (EV-lncRNAs) for hepatocellular carcinoma (HCC). Driver oncogenic lncRNA candidates were selected by a comparative analysis expression profiles from two whole transcriptome human HCC datasets (Catholic_LIHC and TCGA_LIHC). Expression lncRNAs in EVs was evaluated using quantitative reverse transcription PCR. Diagnostic power EV-lncRNAs determined test (n = 44) validation 139)...
Background/Aims: Hepatocellular carcinoma (HCC) is a leading cause of cancer mortality worldwide. Despite identification several biomarkers for HCC diagnosis, challenges such as low sensitivity and intratumoral heterogeneity have impeded early detection, highlighting the need etiology-specific blood biomarkers.Methods: We generated whole-transcriptome sequencing (WTS) targeted proteome data from buffy coat plasma samples patients. By integrating etiological information on viral infection, we...
Liquid biopsy can provide a strong basis for precision medicine. We aimed to identify novel single-nucleotide variants (SNVs) in circulating tumor DNA (ctDNA) patients with hepatocellular carcinoma (HCC). Deep sequencing of plasma-derived ctDNA from 59 HCC was performed using panel 2924 SNVs 69 genes. In 55.9% the patients, at least one somatic mutation detected. Among 25 12 genes, four frequently observed SNVs, MLH1 (13%), STK11 PTEN (9%), and CTNNB1 (4%), were validated droplet digital...
Exosomal microRNAs (exo-miRs) contribute to cancer metastasis. To identify pro-metastatic circulating exo-miRs in hepatocellular carcinoma (HCC), next-generation sequencing-based plasma exo-miR profiles of 14 patients with HCC (eight non-metastatic and six metastasis within 1 year follow-up) were analyzed. Sixty-one miRs significantly overexpressed among metastatic HCC. Candidate selected through integrative analyses two different public expression datasets, GSE67140 The Cancer Genome Atlas...
Cancer-associated fibroblasts (CAFs) contribute to tumor progression, and microRNAs (miRs) play an important role in regulating the tumor-promoting properties of CAFs. The objectives this study were clarify specific miR expression profile CAFs hepatocellular carcinoma (HCC) identify its target gene signatures. Small-RNA-sequencing data generated from nine pairs para-cancer isolated human HCC para-tumor tissues, respectively. Bioinformatic analyses performed HCC-CAF-specific signatures...
Hepatocellular carcinoma (HCC) has a high rate of cancer recurrence (up to 70%) in patients who undergo surgical resection. We investigated prognostic gene signatures for predicting HCC using silico expression analysis. Recurrence-associated candidates were chosen by comparative analysis profiles from two independent whole-transcriptome datasets with underwent Five promising candidate genes, CETN2, HMGA1, MPZL1, RACGAP1, and SNRPB identified, the these genes was evaluated quantitative...
Abstract Background Hepatocellular carcinoma (HCC) is one of the most common and lethal cancers worldwide. Wiskott–Aldrich syndrome protein family member 2 (WASF2) an integral actin cytoskeleton pathway, which plays a crucial role in cell motility. In this study, we aimed to explore WASF2 HCC carcinogenesis its regulatory mechanism. Methods expression was analyzed using six public RNA-seq datasets 66 paired tissues from patients with HCC. The normal hepatocyte phenotypes evaluated...
Abstract Background Hepatocellular carcinoma (HCC) accounts for a majority of primary liver cancer cases and related deaths. The purpose this study was to assess the diagnostic value splicing factor 3b subunit 4 (SF3B4) as novel non-invasive biomarker HCC determine association between SF3B4 expression immune cell infiltration. Methods An enzyme-linked immunosorbent assay (ELISA) used detect levels in plasma samples obtained from healthy controls (HCs) patients with chronic hepatitis,...
Hepatocellular carcinoma (HCC) is known to be lethal disease. However, its prognosis remains poor, primarily because the precise oncogenic mechanisms underlying HCC progression remain elusive, thus hampering effective treatment. Here, we aimed identify potential oncogenes in and elucidate of their action. To candidate genes, an integrative analysis eight publicly available genomic datasets was performed, functional implications identified genes were assessed vitro vivo. Sortilin 1 (SORT1) as...
HCC remains a lethal cancer type, with early detection being critical for improved patient outcomes. This study introduces comprehensive methodological approach to identify the ITGA6 gene as potential blood marker (eHCC) detection. We initially analyzed GSE114564 dataset encompassing various stages of liver disease, identifying 972 differentially expressed genes in HCC. A refined analysis yielded 59 specifically HCC, including ITGA6. Subsequent validation multiple datasets confirmed...
Protein markers of hepatocellular carcinoma (HCC)-derived exosomes (HEX) have not yet been fully evaluated. Here, we identified novel protein contents HEX and their clinical significance as biomarkers. Exosomes were isolated from human HCC cell lines an immortalized normal hepatocyte line. Proteomic analyses revealed 15 markedly overexpressed proteins in HEX. The relevance the was analyzed public RNA-sequencing datasets, 6 selected candidate potential Serum CCT8 CFL1 test cohort (n = 8). In...
Reverse transcription‑quantitative (RT‑q) PCR is the most feasible and useful technique for identifying evaluating cancer biomarkers; however, method requires suitable reference genes gene expression analysis. The aim of present study was to identify normalization relative in human hepatocellular carcinoma (HCC) tissue blood samples. First, 14 candidate were selected through a systematic literature search. levels these (<em>ACTB, B2M, GAPDH, GUSB, HMBS, HPRT1, PGK1, PPIA, RPLP0, RPL13A,...
Purpose : The aim of this study was to obtain single cell-derived clones from human umbilical cord blood (hUCB) derived mesenchymal stem cell (MSC) population, compare the gene expression patterns and differentiation characteristics among hUCB MSC population its monoclonal populations, determine if is homogenous. Materials Methods: cells were isolated a cultured on each well culture plate. pattern expanded detected by RT-PCR for osteogenic, chondrogenic, adipogenic specific genes....
Background: Cancer-associated fibroblasts (CAFs) play an important role in the induction of chemo-resistance. The objectives this study were to clarify mechanism underlying CAF-mediated sorafenib/lenvatinib resistance and identify a novel therapeutic target overcome sorafenib/ lenvatinib hepatocellular carcinoma (HCC). Material methods: Whole transcriptome sequencing (WTS) data nine pairs CAFs para-cancer (PAFs) analyzed key molecules that induce tyrosine kinase inhibitors (TKIs). vitro In...
Abstract Background: Hepatocellular carcinoma (HCC) is one of the most common and lethal cancers worldwide. Wiskott-Aldrich syndrome protein family member 2 (WASF2) an integral actin cytoskeleton pathway that plays a crucial role in cell motility. In this study, we aimed to explore WASF2 HCC carcinogenesis its regulatory mechanism. Methods: expression was analyzed using six public RNA-seq datasets 66 paired tissues from patients with HCC. Role phenotypes evaluated small interfering RNA...