A5017140369- Allergic Rhinitis and Sensitization
- Asthma and respiratory diseases
- Kidney Stones and Urolithiasis Treatments
- Food Allergy and Anaphylaxis Research
- Sinusitis and nasal conditions
- Drug-Induced Adverse Reactions
- Gut microbiota and health
- Urinary Bladder and Prostate Research
- Probiotics and Fermented Foods
- Transgenic Plants and Applications
- Inhalation and Respiratory Drug Delivery
- Respiratory and Cough-Related Research
- Pediatric Urology and Nephrology Studies
- Ocular Surface and Contact Lens
- Adenosine and Purinergic Signaling
- Advanced Drug Delivery Systems
- Nanoparticle-Based Drug Delivery
- Plant Reproductive Biology
- Esophageal and GI Pathology
- Biliary and Gastrointestinal Fistulas
- Immune Cell Function and Interaction
- Paleopathology and ancient diseases
- Connexins and lens biology
- Exercise and Physiological Responses
- Olfactory and Sensory Function Studies
University of Chicago
2011-2023
RELX Group (United States)
2018
Harvard University
2002-2004
Massachusetts General Hospital
2002-2004
Saarland University
1997
Hôpital Arnaud de Villeneuve
1996
Abstract The mechanisms by which signaling the innate immune system controls susceptibility to allergy are poorly understood. In this report, we show that intragastric administration of a food allergen with mucosal adjuvant induces allergen-specific IgE, elevated plasma histamine levels, and anaphylactic symptoms in three different strains mice lacking functional receptor for bacterial LPS (Toll-like 4 (TLR4)), but not MHC-matched or congenic controls. Susceptibility correlates Th2-biased...
The microbiome modulates host immunity and aids the maintenance of tolerance in gut, where microbial food-derived antigens are abundant. Yet modern dietary factors excessive use antibiotics have contributed to rising incidence food allergies, inflammatory bowel disease other non-communicable chronic diseases associated with depletion beneficial taxa, including butyrate-producing Clostridia. Here we show that intragastrically delivered neutral negatively charged polymeric micelles releasing...
Abstract Although helminths induce a polarized Th2 response they have been shown, in clinical studies, to confer protection against allergies. To elucidate the basis for this paradox, we examined influence of an enteric helminth infection on model food allergy. Upon Ag challenge, mice fed peanut (PN) extract plus mucosal adjuvant cholera toxin (CT) produced PN-specific IgE that correlated with systemic anaphylactic symptoms and elevated plasma histamine. was not induced helminth-infected PN...
Hyperoxaluria is a major risk factor for kidney stones and has no specific therapy, although Oxalobacter formigenes colonization associated with reduced stone risk. O. interacts colonic epithelium induces oxalate secretion, thereby reducing urinary excretion, via an unknown secretagogue. The difficulties in sustaining underscore the need to identify derived factors inducing secretion. We therefore evaluated effects of culture conditioned medium (CM) on apical 14 C-oxalate uptake by human...
Pollen grains are the male gametophytes that deliver sperm cells to female during sexual reproduction of higher plants. is a major source aeroallergens and environmental antigens. The pollen coat harbors plethora lipids required for hydration, germination, penetration stigma by tubes. In addition proteins, displays wide array interact with human immune system. Prior searches allergens have focused on identification intracellular allergenic but largely overlooked much extracellular matrix,...
Background The pollen coat is the first structure of to encounter mucosal immune system upon inhalation. Prior characterizations allergens have focused on water-soluble, cytoplasmic proteins, but overlooked much extracellular coat. Due washing with organic solvents when prepared, these proteins are typically absent from commercial standardized allergenic extracts (i.e., "de-fatted"), and, as a result, their involvement in allergy has not been explored. Methodology/Principal Findings Using...
The 2S albumin storage protein of <i>Ricinus communis</i> consists the two heterodimeric proteins Ric c 1 and 3 each which is composed a small large subunit linked together by disulphide bridges. complete primary structures both were determined enzymatic degradation automated Edman degradation. sequences all four chains correspond to known cDNA sequence gene presumed precursor molecule previously partial for 3. In addition, few differences in amino acid positions found seem be...
Most kidney stones (KS) are composed of calcium oxalate and small increases in urine enhance the stone risk. Obesity is a risk factor for KS, urinary excretion with increased body size. We previously established obese ob/ob ( ob) mice as model (3.3-fold higher oxalate) to define pathogenesis obesity-associated hyperoxaluria (OAH). The purpose this study was test hypothesis that enhanced intestinal paracellular permeability contributes OAH by increasing passive absorption. ob Mice have...
Most kidney stones are composed of calcium oxalate, and small increases in urine oxalate enhance the stone risk. The mammalian intestine plays a crucial role homeostasis, we had recently reported that Oxalobacter-derived factors stimulate transport by human intestinal Caco2-BBE (C2) cells through PKA activation. We therefore evaluated whether is directly regulated activation signaling pathway. To this end, was activated with forskolin IBMX (F/I). F/I significantly stimulated (3.7-fold) [ 14...
Background Systemic corticosteroids are the most effective anti‐inflammatory drugs used for controlling chronic rhinosinusitis (CRS) symptoms. The potential mechanisms their beneficial effects include increasing number and function of T regulatory cells (Tregs), as reported in local tissue post–intranasal steroid treatment. We investigated effect systemic on peripheral blood (PB) Tregs subjects with CRS. Methods Twenty CRS 19 controls were recruited. PB mononuclear (PBMCs) isolated from...
Most kidney stones (KS) are composed of calcium oxalate, and small increases in urine oxalate affect the stone risk. Intestinal secretion mediated by anion exchanger SLC26A6 (PAT1) plays a crucial role limiting net absorption ingested thereby preventing hyperoxaluria related KS, reflecting importance understanding regulation intestinal transport. We previously showed that ATP UTP inhibit transport human Caco2-BBE cells (C2). Since is rapidly degraded to adenosine (ADO), we examined whether...
Background Nasal allergen challenge (NAC) leads to a nasal ocular reflex, which is augmented by allergic inflammation. This study was designed confirm our previous observation that an intranasal steroid inhibits the reflex and show histamine does not play important role in genesis of this reflex. Methods We performed randomized, double-blind, double-dummy, placebo (PL)-controlled, four-way crossover trial subjects with seasonal rhinitis out season. Subjects were randomized receive 1 week...
Kidney stones (KSs) are very common, excruciating, and associated with tremendous healthcare cost, chronic kidney disease (CKD), failure (KF). Most KSs composed of calcium oxalate small increases in urinary concentration significantly enhance the stone risk. Oxalate also potentially contributes to CKD progression, disease-associated cardiovascular diseases, poor renal allograft survival. This emphasizes urgent need for plasma lowering therapies, which can be achieved by enhancing enteric...
Abstract The microbiome modulates host immunity and aids in maintenance of tolerance the gut, where microbial food-derived antigens are abundant. Modern lifestyle practices, including diet antibiotic use, have depleted beneficial taxa, specifically butyrate-producing Clostridia. This depletion is associated with rising incidence food allergy, inflammatory bowel diseases, other noncommunicable chronic diseases. Although butyrate known to play important roles regulating gut maintaining...
Abstract Regulatory T cells (Tregs) play essential roles in maintaining immune homeostasis and preventing autoimmunity allergy. Peripherally derived Tregs are induced most efficiently the lymph nodes (LNs), yet current therapies not effectively delivered to these sites. The short-chain fatty acid butyrate facilitates extrathymic differentiation of has been considered a promising therapeutic candidate. However, there challenges its use, including foul odor, quick metabolism, low efficiency...