A5006728349- Neurogenesis and neuroplasticity mechanisms
- Neuroinflammation and Neurodegeneration Mechanisms
- Cancer-related molecular mechanisms research
- Single-cell and spatial transcriptomics
- Cell Image Analysis Techniques
- RNA Research and Splicing
- Neuroscience and Neuropharmacology Research
- Glioma Diagnosis and Treatment
- Epigenetics and DNA Methylation
- Neural dynamics and brain function
- Axon Guidance and Neuronal Signaling
- MicroRNA in disease regulation
- Bioinformatics and Genomic Networks
- Neural Networks and Applications
- RNA modifications and cancer
- Genetics and Neurodevelopmental Disorders
- Advanced Fluorescence Microscopy Techniques
- Prenatal Screening and Diagnostics
- Exercise and Physiological Responses
- Dermatology and Skin Diseases
- Pluripotent Stem Cells Research
- Developmental Biology and Gene Regulation
- Photoreceptor and optogenetics research
- Receptor Mechanisms and Signaling
- Microtubule and mitosis dynamics
BioMarin (United States)
2024
Stanford University
2017-2021
Howard Hughes Medical Institute
2017-2021
Broad Center
2010-2017
University of California, San Francisco
2011-2017
University of California San Francisco Medical Center
2011-2014
University of Chicago
2013
Long non-coding RNAs (lncRNAs) comprise a diverse class of transcripts that can regulate molecular and cellular processes in brain development disease. LncRNAs exhibit cell type- tissue-specific expression, but little is known about the expression function lncRNAs developing human brain. Furthermore, it has been unclear whether are highly expressed subsets cells within tissues, despite appearing lowly bulk populations. We use strand-specific RNA-seq to deeply profile from polyadenylated...
The dramatic increase in neocortical size and folding during mammalian brain evolution has been attributed to the elaboration of subventricular zone (SVZ) associated neural progenitors. However, recent studies have shown that SVZ abundance resident progenitors do not directly predict cortical topography, suggesting complex behaviors themselves may contribute overall shape adult cortex. Using time-lapse imaging, we examined dynamic ferret, a gyrencephalic carnivore, focusing our analysis on...
Evolutionary expansion of the human neocortex is partially attributed to a relative abundance neural stem cells in fetal brain called outer radial glia (oRG). oRG display characteristic division mode, mitotic somal translocation (MST), which soma rapidly translocates toward cortical plate immediately prior cytokinesis. MST may be essential for progenitor zone expansion, but mechanism unknown, hindering exploration its function development and disease. Here, we show that requires activation...
The projection targets of a neuronal population are key feature its anatomical characteristics. Historically, tissue sectioning, confocal microscopy, and manual scoring specific regions interest have been used to generate coarse summaries mesoscale projectomes. We present here TrailMap, three-dimensional (3D) convolutional network for extracting axonal projections from intact cleared mouse brains imaged by light-sheet microscopy. TrailMap allows region-based quantification total axon content...
Angelman syndrome (AS), an early-onset neurodevelopmental disorder characterized by abnormal gait, intellectual disabilities, and seizures, occurs when the maternal allele of UBE3A gene is disrupted, since paternal silenced in neurons antisense (UBE3A-AS) transcript. Given importance early treatment, we hypothesized that prenatal delivery oligonucleotide (ASO) would downregulate murine Ube3a-AS, resulting increased protein functional rescue. Using a mouse model with Ube3a-YFP reports...
Mammalian medial and lateral hippocampal networks preferentially process spatial- object-related information, respectively. However, the mechanisms underlying assembly of such parallel during development remain largely unknown. Our study shows that, in mice, complementary expression cell surface molecules teneurin-3 (Ten3) latrophilin-2 (Lphn2) networks, respectively, guides precise CA1-to-subiculum connections both networks. In network, Ten3-expressing (Ten3+) CA1 axons are repelled by...
Pollen grains are the male gametophytes that deliver sperm cells to female during sexual reproduction of higher plants. is a major source aeroallergens and environmental antigens. The pollen coat harbors plethora lipids required for hydration, germination, penetration stigma by tubes. In addition proteins, displays wide array interact with human immune system. Prior searches allergens have focused on identification intracellular allergenic but largely overlooked much extracellular matrix,...
The neocortex of primates, including humans, contains more abundant and diverse inhibitory neurons compared with rodents, but the molecular foundations these observations are unknown. Through integrative gene coexpression analysis, we determined a consensus transcriptional profile GABAergic in mid-gestation human neocortex. By comparing this to genes expressed purified from neonatal mouse neocortex, identified conserved distinct aspects expression cells between species. We show here that...
Abstract Parallel information processing is a salient feature of complex nervous systems. For example, the medial and lateral hippocampal networks (MHN LHN) preferentially process spatial- object-related information, respectively. However, mechanisms underlying parallel network assembly during development remain largely unknown. Here, we show that complementary expression cell-surface molecules Teneurin-3 (Ten3) Latrophilin-2 (Lphn2) in MHN LHN, respectively, guides precise both LHN....
Abstract The projection targets of a neuronal population are key feature its anatomical characterization. Historically, tissue sectioning, confocal microscopy, and manual scoring specific regions interest have been used to generate coarse summaries mesoscale projectomes. We present here TrailMap, 3D convolutional network for extracting axonal projections from intact cleared mouse brains imaged by light-sheet microscopy. TrailMap allows region-based quantification total axon content in large...
Single-cell transcriptomics has been widely applied to classify neurons in the mammalian brain, while systems neuroscience historically analyzed encoding properties of cortical without considering cell types. Here we examine how specific transcriptomic types mouse prefrontal cortex (PFC) projection relate axonal projections and across multiple cognitive tasks. We found that most projected targets, targets received from types, except PFC→PAG (periaqueductal gray). By comparing Ca2+-activity...
A Correction to this paper has been published: https://doi.org/10.1038/s41593-020-00779-0.