A5082580301- RNA Interference and Gene Delivery
- Immunotherapy and Immune Responses
- Biopolymer Synthesis and Applications
- 3D Printing in Biomedical Research
- Immune Cell Function and Interaction
- Advanced biosensing and bioanalysis techniques
- HIV Research and Treatment
- Tissue Engineering and Regenerative Medicine
- Electrospun Nanofibers in Biomedical Applications
- Advanced Drug Delivery Systems
- Additive Manufacturing and 3D Printing Technologies
- Nanoparticle-Based Drug Delivery
- biodegradable polymer synthesis and properties
- Pluripotent Stem Cells Research
- Immune Response and Inflammation
- Polymer Surface Interaction Studies
- Surface Modification and Superhydrophobicity
- Antimicrobial agents and applications
- Wound Healing and Treatments
- Neuroscience and Neural Engineering
- Influenza Virus Research Studies
- Advancements in Transdermal Drug Delivery
- Nanofabrication and Lithography Techniques
- Cancer Research and Treatments
- Advanced Sensor and Energy Harvesting Materials
Osaka University
2014-2023
Ube Frontier University
2017-2021
Japan Science and Technology Agency
2007-2016
Shibuya (Japan)
2014
Graduate School USA
2014
Suita Municipal Hospital
2014
Kagoshima University
1997-2010
Shibaura Institute of Technology
2007
JIMRO (Japan)
2002-2005
Abstract Nanoparticles are considered to be efficient tools for inducing potent immune responses by an Ag carrier. In this study, we examined the effect of Ag-carrying biodegradable poly(γ-glutamic acid) (γ-PGA) nanoparticles (NPs) on induction in mice. The NPs were efficiently taken up dendritic cells (DCs) and subsequently localized lysosomal compartments. γ-PGA strongly induced cytokine production, up-regulation costimulatory molecules, enhancement T cell stimulatory capacity DCs. These...
This review describes the preparation of core-corona type polymeric nanoparticles and their applications in various technological biomedical fields. Over past two decades, we have studied synthesis clinical composed hydrophobic polystyrene hydrophilic macromonomers. These were utilized as catalyst carriers, carriers for oral peptide delivery, virus capture agents, vaccine so on. Moreover, based on this research, attempted to develop novel biodegradable poly(γ-glutamic acid) (γ-PGA)...
Some neuropsychiatric disease, including schizophrenia, may originate during prenatal development, following periods of gestational hypoxia and placental oxidative stress. Here we investigated if promotes damaging secretions from the placenta that affect fetal development whether a mitochondria-targeted antioxidant MitoQ might prevent this. Gestational caused low birth-weight changes in young adult offspring brain, mimicking those human disease. Exposure cultured neurons to plasma or model...
Autologous split-thickness skin grafts are the preferred treatment for excised burn wounds, but donor sites autografting often limited in patients with extensive burns. A number of alternative treatments already use to treat large burns and ulcers. Despite intense efforts develop tissue-engineered skin, delayed or absent vascularization is one major reasons engraftment failure. To overcome these problems, we developed a scaffold-free 3-dimensional (3D) substitute containing vascular networks...
Herein, we report the fabrication of native organ-like three-dimensional (3D) cardiac tissue with an oriented structure and vascular network using a layer-by-layer (LbL), cell accumulation 3D printing technique for regenerative medicine pharmaceutical applications. We firstly evaluated shaping ability hydroxybutyl chitosan (HBC), thermoresponsive polymer, by robotic dispensing printer. Next, tried to fabricate orientation-controlled human induced pluripotent stem cell-derived cardiomyocytes...
Amphiphilic graft copolymers consisting of poly(γ-glutamic acid) (γ-PGA) as the hydrophilic backbone and l-phenylalanine ethylester (L-PAE) hydrophobic side chain were synthesized by grafting L-PAE to γ-PGA. The nanoparticles prepared a precipitation method, about 200 nm-sized obtained due their amphiphilic properties. hydrolytic enzymatic degradation these γ-PGA was studied gel permeation chromatography (GPC), scanning electron microscopy (SEM), dynamic light scattering (DLS) 1H NMR...
Antigen delivery systems using polymeric nanoparticles are of special interest as stable protein-based antigen carriers. In the present study, novel biodegradable poly(gamma-glutamic acid) (gamma-PGA) were examined for their and immunostimulatory activities in vitro vivo. The uptake ovalbumin by dendritic cells was markedly enhanced gamma-PGA nanoparticles, gradually released from into cells. addition, appeared to have great potential an adjuvant, because they could induce maturation...
Amphiphilic block or graft copolymers have been demonstrated to form a variety of self-assembled nano/microstructures in selective solvents. In this study, the self-association behavior biodegradable composed poly(γ-glutamic acid) (γ-PGA) as hydrophilic segment and L-phenylalanine (Phe) hydrophobic aqueous solution was investigated. The association unimer nanoparticle formation these γ-PGA-graft-Phe (γ-PGA-Phe) were characterized with focus on effect Phe grafting degree intra- interpolymer...
Abstract Layer-by-Layer (LbL) assembly of interactive polymers onto surfaces leads to the construction multilayered ultrathin films, which can be done simply by alternately dipping substrate into various solutions. The range applications this LbL broadened introducing molecular recognition mechanisms for and proteins, using weak interactions such as van der Waals biological recognition. As a specific example, it applied formation stereocomplexes poly(methyl methacrylate) (PMMA), poly-lactide...
We report the design and preparation of polyion complex (PIC) nanoparticles composed anionic hydrophobically modified cationic poly(amino acid) effect hydrophobic interactions on stability these PIC under physiological conditions. selected poly(γ-glutamic (γ-PGA) as biodegradable polymer poly(ε-lysine) (ε-PL) polymer. Amphiphilic graft copolymers consisting γ-PGA l-phenylalanine (l-Phe) side chain were synthesized by grafting l-Phe to γ-PGA. The prepared mixing γ-PGA-graft-l-Phe (γ-PGA-Phe)...
Abstract Size‐regulated amphiphilic poly(amino acid) nanoparticles (NPs) composed of poly( γ ‐glutamic ( ‐PGA) and the hydrophobic amino acid derivative, L ‐phenylalanine ethyl ester (Phe) are prepared to evaluate effects particle size on dendritic cell (DC) uptake NPs their immune stimulatory activities as delivery carriers adjuvants. The Phe‐conjugated ‐PGA ‐PGA–Phe NPs) is easily controlled by regulating aggregated numbers. Each differently sized could efficiently encapsulate ovalbumin...
Jet set: Layer-by-layer alternate stepwise deposition of poly(L-lactide) (PLLA) and poly(D-lactide) (PDLA) is used to fabricate a polylactide (PLA) stereocomplex on substrate (see picture). Multiple inkjet passes improve the crystal structure PLA composite without intermediate rinsing steps.