A5101995736- Sulfur Compounds in Biology
- Helicobacter pylori-related gastroenterology studies
- Pulmonary Hypertension Research and Treatments
- Galectins and Cancer Biology
- Veterinary medicine and infectious diseases
- Eicosanoids and Hypertension Pharmacology
- Neonatal Respiratory Health Research
- Fatty Acid Research and Health
- Nutritional Studies and Diet
- Advanced Chemical Sensor Technologies
- Connective Tissue Growth Factor Research
- Vector-Borne Animal Diseases
- Osteoarthritis Treatment and Mechanisms
- Building materials and conservation
- Immunotherapy and Immune Responses
- Ion channel regulation and function
- Food Allergy and Anaphylaxis Research
- Immune Response and Inflammation
- Medical Research and Treatments
- Regional Development and Environment
- Protein Kinase Regulation and GTPase Signaling
- Renin-Angiotensin System Studies
- Pancreatic function and diabetes
- Sodium Intake and Health
- Chronic Obstructive Pulmonary Disease (COPD) Research
Northern Jiangsu People's Hospital
2025
China Academy of Building Research
2024
Beijing Children’s Hospital
2022
Capital Medical University
2022
Peking University First Hospital
2015-2019
Peking University
2015-2019
University of South China
2008-2013
National Cheng Kung University
2012
Abstract Background: Pulmonary artery endothelial cell (PAEC) inflammation is a critical event in the development of pulmonary arterial hypertension (PAH). However, pathogenesis PAEC remains unclear. Methods: Purified recombinant human inhibitor κB kinase subunit β (IKKβ) protein, PAECs and monocrotaline-induced hypertensive rats were employed study. Site-directed mutagenesis, gene knockdown or overexpression conducted to manipulate expression activity target protein. Results: We showed that...
Background . The study was designed to investigate if H 2 S could inhibit high‐salt diet‐induced renal excessive oxidative stress and kidney injury in Dahl rats. Methods Male salt‐sensitive SD rats were used. Blood pressure (BP), serum creatinine, urea, creatinine clearance rate, 24‐hour urine protein measured. Renal ultra‐ microstructures observed. Collagen‐I ‐III contents the oxidants antioxidants levels tissue detected. Keap1/Nrf2 association Keap1 s‐sulfhydration Results After 8 weeks of...
The present study was designed to investigate whether endogenous sulphur dioxide (SO2) controlled pulmonary inflammation in a rat model of oleic acid (OA)-induced acute lung injury (ALI). In this model, adenovirus expressing aspartate aminotransferase (AAT) 1 delivered the lungs, and levels SO2 proinflammatory cytokines tissues were measured. human alveolar epithelial cell line A549, nuclear translocation DNA binding activities wild-type (wt) C38S (cysteine-to-serine mutation at p65 Cys38)...
The study was designed to examine if the vasorelaxant effect of hydrogen sulfide mediated by sulfhydration-associated phosphodiesterase (PDE) 5A dimerization. thoracic aorta rat separated and effects were examined with in vitro vascular perfusion experiments. dimerization sulfhydration PDE soluble guanylatecyclase (sGC) measured. protein kinase G (PKG) activities tested. Intracellular cGMP content detected enzyme-linked immunosorbent assay (ELISA). results showed that NaHS relaxed isolated...
The study aimed to examine the protective effect of hydrogen sulfide (H2S) on high-salt-induced oxidative stress and myocardial hypertrophy in salt-sensitive (Dahl) rats. Thirty male Dahl rats 40 SD were included study. They randomly divided into control (Dahl + NS), high salt HS), HS NaHS, NS, HS, NaHS hydroxylamine (HA). Rats NS groups given chow with 0.5% NaCl 0.9% normal saline intraperitoneally daily. Myocardial structure, α-myosin heavy chain (α-MHC) β-myosin (β-MHC) expressions...
The aim of the study was to investigate role endogenous sulfur dioxide (SO2)/ aspartate aminotransferase 1 (AAT1) pathway in stretch-induced excessive collagen expression and its mechanism. mechanical stretch downregulated SO2/AAT1 increased I III protein expression. Importantly, AAT1 overexpression blocked increase expression, transforming growth factor-β1 (TGF- β1) phosphorylation Smad2/3 induced by stretch, but knockdown mimicked TGF- β1 stretch. Mechanistically, SB431542, a...
Background We aimed to explore the role of endogenous sulfur dioxide ( SO 2 ) in pulmonary vascular collagen remodeling induced by monocrotaline and its mechanisms. Methods Results A rat model monocrotaline‐induced was developed administered with l ‐aspartate‐β‐hydroxamate or donor. The morphology small arteries metabolism were examined. Cultured arterial fibroblasts stimulated transforming growth factor β1 TGF ‐β1) used mechanism. results showed that monocrotaline‐treated rats, mean artery...
We aimed to investigate the regulatory effects of hydrogen sulfide (H2S) on carotid sinus baroreceptor sensitivity and its mechanisms. Male Wistar-Kyoto rats spontaneously hypertensive (SHRs) were used in experiment given an H2S donor or a cystathionine-β-synthase inhibitor, hydroxylamine, for 8 weeks. Systolic blood pressure cystathionine-β-synthase/H2S pathway detected. Carotid functional curve analyzed using isolated perfusion technique. Effects transient receptor potential cation channel...
Vacuolating cytotoxin (VacA) is an important virulence factor in the pathogenesis of Helicobacter pylori-related diseases. The aim this study was to investigate function amino-terminal 476 residue fragment (p52) VacA and possible molecular mechanisms responsible for its induction proinflammatory cytokines secretion apoptosis. Human acute monocytic leukemia cell line THP-1 used as vitro model apoptosis induced by transfection a recombinant plasmid encoding VacA. results showed that p52...
Both hydrogen sulfide (H2S) and sulfur dioxide (SO2) are produced endogenously from the mammalian metabolic pathway of sulfur-containing amino acids play important roles in several vascular diseases. However, their interaction during control function has not been fully clear. Here, we investigated potential role H2S SO2 production regulation vivo vitro. Wistar rats were divided into vehicle, SO2, DL-propargylglycine (PPG) + β-cyano-L-alanine (BCA) sodium hydrosulfide (NaHS) groups. donor was...
Allantoin, an active principle of the yam, belongs to group guanidinium derivatives and has been reported lower plasma glucose in diabetic animals. Recent evidence indicates that activation imidazoline I<sub>2B</sub> receptor (I<sub>2B</sub>R) by also increases uptake; however, effect allantoin on I<sub>2B</sub>R is still unknown. Glucose uptake into cultured C<sub>2</sub>C<sub>12</sub> cells was determined using 2-[<sup>14</sup>C]-deoxy-<sc>d</sc>-glucose as a tracer. The changes...
Timber-framed masonry structures are widely used around the world, and their seismic performance is generally poor. Most of them have not been seismically strengthened. In areas with high fortification intensity, there great potential safety hazards. And it urgent to carry out effective reinforcement. However, due complicated construction process existing reinforcement technology, poor durability materials, significant disturbance life original residents, an efficient single-story...
Since the implementation of rural vitalization strategy, how to set scientific and reasonable evaluation indicators evaluate results has been great theoretical practical significance. This article, at national, provincial regional level respectively, applies entropy create method for determining weight parallel indicators, establish basic sub-indicators, determine different combinations through official data survey data. According research findings, whole country provinces have all developed...
Objective To investigate the production of hydrogen sulfide (H2S) in rats with monocrotaline (MCT)-induced pulmonary hypertension(PAH). Methods Fourteen male Wistar were randomly divided into 2 groups (7 cases for each group): control group and MCT group.Rats intraperitoneally injected (60 mg/kg) on day 1 while received only same volume 9 g/L saline.And then conventional breeding was given 21 days.Mean artery pressure (mPAP) evaluated via right cardiac catheterization procedure.The...
Objective To investigate the immune response of mucosal immunization new chitosan(CS) nanoparticles coating DNA vaccine. Methods The chitosan containing plasmid encoding H. pylori lipoprotein Lpp20 gene were prepared using a complex coacervation method and then its speciality analyzed. We administered naked chitosan-DNA to 6-week-old female BALB/c mice by intranasal or oral routes observe humoral cellular responses. Results Naked pcDNA3.1 ( + )/Lpp20 chitosan-pcDNA3. 1 both induced effective...
Objective To study the effect of VacA on secretion THP-1 macrophages as an individual virulence determinant, and NF-kB macrophages. Methods The recombinant plasmid pDsRed-Monomer-Cl/vacA was transfected into cytokine con-tent TNF-α or IL-1β in culture medium tested quantitatively with ELISA kit, respectively. content NO ROS Griess reagent DCFH-DA fluorescent probe. apoptosis rate by flow cytometry. PDTC, inhibitor NF-kB, plasmids, also studied. activity examined cells electrophoretic...
Helicobacter pylori is a frequent aetiological agent all over the world,and thrives in human stomach an environment of varying acidity.This microorganism inevitably associated with gastritis and frequently peptic ulcer or gastric cancer.Recent researches indicate that mechanism acid acclimation H.priori depends on UreI membrane protein.Many studies have been done role protein acclimation,all these H.pylori.In article,the gene structure,its ale reviewed. Key words: Helicobacter...