A5074659798- Particle physics theoretical and experimental studies
- Quantum Chromodynamics and Particle Interactions
- Neutrino Physics Research
- Astrophysics and Cosmic Phenomena
- Enzyme Structure and Function
- Enzyme Production and Characterization
- High-Energy Particle Collisions Research
- Studies on Chitinases and Chitosanases
- Biofuel production and bioconversion
- Radiation Detection and Scintillator Technologies
- Advanced Cellulose Research Studies
- Atomic and Subatomic Physics Research
- Advanced Electron Microscopy Techniques and Applications
- Nuclear Physics and Applications
- Sinusitis and nasal conditions
- Nuclear physics research studies
- Asthma and respiratory diseases
- Plant Parasitism and Resistance
- Protein Structure and Dynamics
- Salivary Gland Tumors Diagnosis and Treatment
- ATP Synthase and ATPases Research
- Asymmetric Synthesis and Catalysis
- Superconducting Materials and Applications
- Allergic Rhinitis and Sensitization
- Analytical Chemistry and Chromatography
Institute for Molecular Science
2017-2025
Shizuoka University
2020-2025
National Institutes of Natural Sciences
2016-2025
Hokkaido University
2024
Okayama University of Science
2019-2023
Osaka Prefectural Institute of Public Health
2006-2023
Tohoku University
2012-2022
The Nakamura Hajime Eastern Institute
2017-2022
Foundation Osaka City Museum Association
2022
Nakamura Memorial Hospital
2020-2022
OBJECTIVE—Interleukin (IL)-18 is a proinflammatory cytokine secreted from mononuclear cells. Serum concentration of IL-18 strong predictor death in patients with cardiovascular diseases. Recent studies have shown that microinflammation involved the pathogenesis diabetic nephropathy as well This study aimed to test hypothesis serum level common and atherosclerosis type 2 diabetes. RESEARCH DESIGN AND METHODS—Eighty-two Japanese diabetes 55 age- sex-matched healthy control subjects were...
Although C–H oxidation of hydrocarbons is generally difficult, allylic relatively simple and predictable, even on a preparative scale, because active species generated at the position are stabilized by double bond. Therefore, has been employed in natural product synthesis, variety reagents conditions for have reported. However, suitable synthesis limited terms efficiency chemo-, regio-, stereoselectivity, owing to structural characteristic diversity products. This review addresses...
Hydrolysis of carbohydrates is a major bioreaction in nature, catalyzed by glycoside hydrolases (GHs). We used neutron diffraction and high-resolution x-ray analyses to investigate the hydrogen bond network inverting cellulase PcCel45A, which an endoglucanase belonging subfamily C GH family 45, isolated from basidiomycete Phanerochaete chrysosporium. Examination enzyme enzyme-ligand structures indicates key role multiple tautomerizations asparagine residues peptide bonds, are finally...
A thermostable enzyme PET2, found in a metagenome library, has been engineered to improve its hydrolytic activity against polyethylene terephthalate (PET). The PET2 wild-type (WT) showed melting temperature of 69.0 °C and produced water-soluble reaction products at rate 0.40 min–1 (2.4 μM from 0.1 after 60 min reaction) an amorphous PET film °C. Mutations for surface charge modification, backbone stabilization, formation additional disulfide bond were introduced into the WT, best mutant...
Background: Most diseases are thought to arise from interactions between environmental factors and the host genotype. To detect gene–environment in development of lifestyle-related diseases, especially cancer, Japan Multi-institutional Collaborative Cohort (J-MICC) Study was launched 2005.Methods: We initiated a cross-sectional study examine associations genotypes with lifestyle clinical factors, as assessed by questionnaires medical examinations. The 4519 subjects were selected among...
Analysis of heterogeneous catalysis at an interface is difficult because the variety reaction sites and difficulty observing reaction. Enzymatic hydrolysis cellulose by cellulases a typical solid/liquid interface, key parameter such reactions on polymeric substrates processivity, i.e., number catalytic cycles that can occur without detachment enzyme from substrate. In this study, we evaluated three closely related glycoside hydrolase family 7 cellobiohydrolases filamentous fungi molecular...
Mutation of Trp-40 in the Cel7A cellobiohydrolase from Trichoderma reesei (TrCel7A) causes a loss crystalline cellulose-degrading ability.Mutant W40A showed reduced specific activity for cellulose and diffused chain entrance active site tunnel.Trp-40 is essential end loading to initiate processive hydrolysis TrCel7A.The mechanisms polysaccharide degradation are clarified. The glycoside hydrolase family 7 one best studied cellulases with ability degrade highly cellulose. catalytic domain...
Processive chitinase is a linear molecular motor which moves on the surface of crystalline chitin driven by processive hydrolysis single chain. Here, we analyse mechanism underlying unidirectional movement Serratia marcescens A (SmChiA) using high-precision single-molecule imaging, X-ray crystallography, and all-atom dynamics simulation. SmChiA shows fast ~50 nm s-1 with 1 forward backward steps, consistent length reaction product chitobiose. Analysis kinetic isotope effect reveals...
Efficient depolymerization of crystalline cellulose requires cooperation between multiple cellulolytic enzymes. Through biochemical approaches, molecular dynamics (MD) simulation, and single-molecule observations using high-speed atomic force microscopy (HS-AFM), we quantify track synergistic activity for cellobiohydrolases (CBHs) with a lytic polysaccharide monooxygenase (LPMO) from Phanerochaete chrysosporium. Increasing concentrations LPMO (AA9D) increased the glycoside hydrolase family 6...
Karrikin Insensitive 2 (KAI2), identified as the receptor protein for karrikins (KARs), which are smoke-derived seed germination stimulants, belongs to same α/β-hydrolase family D14, strigolactones (SLs). KAI2 is believed recognize an endogenous butenolide (KAI2 ligand; KL), but identity of this compound remains unknown. Recent studies have suggested that ligand hydrolysis by a prerequisite activation induce interaction with target proteins, similar situation D14. However, direct...
ADVERTISEMENT RETURN TO ISSUEPREVArticleNEXTHighly diastereoselective reactions of ytterbium-mediated alkynyllithium and alkynylmagnesium reagents with chiral 2-acyl-1,3-oxathianes: reversal diastereoselectivityKiitiro Utimoto, Akihiko Nakamura, Seijiro MatsubaraCite this: J. Am. Chem. Soc. 1990, 112, 22, 8189–8190Publication Date (Print):October 1, 1990Publication History Published online1 May 2002Published inissue 1 October 1990https://doi.org/10.1021/ja00178a066RIGHTS & PERMISSIONSArticle...
Trichoderma reesei Cel6A (TrCel6A) is a cellobiohydrolase that hydrolyzes crystalline cellulose into cellobiose. Here we directly observed the reaction cycle (binding, surface movement, and dissociation) of single-molecule intact TrCel6A, isolated catalytic domain (CD), cellulose-binding module (CBM), CBM linker (CBM-linker) on Iα. The CBM-linker showed binding rate constant almost half whereas those CD were only one-tenth TrCel6A. These results indicate glycosylated region largely...
Cellulose is the most abundant biomass on Earth, and many microorganisms depend it as a source of energy. It consists mainly crystalline amorphous regions, natural degradation part highly dependent degree processivity degrading enzymes (i.e., extent continuous hydrolysis without detachment from substrate cellulose). Here, we report high-speed atomic force microscopic (HS-AFM) observations movement four types cellulases derived cellulolytic bacteria Cellulomonas fimi various insoluble...
ABSTRACT Nitrification by heterotrophic microorganisms is an important part of the nitrogen cycle in environment. The enzyme responsible for core function nitrification pyruvic oxime dioxygenase (POD). POD a non-heme, Fe(II)-dependent that catalyzes dioxygenation to produce pyruvate and nitrite. To analyze catalytic mechanism POD, crystal structure from Alcaligenes faecalis (AfPOD) was determined at 1.76 Å resolution. homotetramer, subunit homologous those class II aldolases, particular,...
The methanolic extract from the fruit of Citrullus colocynthis showed an inhibitory effect on ear passive cutaneous anaphylaxis reactions as a type I allergic model in mice. From extract, two new cucurbitane-type triterpene glycosides, colocynthosides A and B, were isolated together with 17 known constituents. structures B elucidated basis chemical physicochemical evidence. In addition, principal glycoside, cucurbitacin E 2-O-β-D-glucopyranoside, its aglycon, E, exhibited antiallergic...
Eosinophilic chronic rhinosinusitis (ECRS) is a subtype of associated with asthma. CD69 an important marker activation for eosinophils. But, whether correlation exist between the expression on eosinophils and clinical findings unclear. We performed quantitative PCR and/or flow cytometry using tissue purified from blood nasal polyps 12 patients ECRS 8 without (controls). assessed including polyp (NP) scores, sinus CT findings, pulmonary function test results, examined their possible...
Clinical data of esaxerenone in hypertensive patients with diabetic kidney disease (DKD) are lacking. We evaluated the efficacy and safety DKD an inadequate response to blood pressure (BP)-lowering treatment. In this multicenter, open-label, prospective study, were divided into urinary albumin-to-creatinine ratio subcohorts (UACR < 30, 30 300, 300 1000 mg/gCr). Esaxerenone was initiated at 1.25 mg/day followed by incremental dose escalation based on BP serum potassium level monitoring. The...
The total cross sections were measured for coherent double neutral-pion photoproduction on the deuteron at incident energies below 0.9 GeV first time. No clear resonance-like behavior is observed in excitation function Wγd=2.38–2.61 GeV, where d⁎(2380) dibaryon resonance COSY expected to appear. consistent with existing theoretical calculation this reaction. upper limit of section found be 0.034μb Wγd=2.37 (90% confidence level) γd→π0π0d
Gold nanoparticles (AuNPs) have been used as a contrast agent for optical imaging of various single biomolecules. Because AuNPs high scattering efficiency without photobleaching, biomolecular dynamics observed with nanometer localization precision and sub-millisecond time resolution. To understand the working principle motors in greater detail, further improvement resolution is necessary. Here, we investigated lower limit achievable fundamental law, which determines precision. We first...