A5007879499- 3D Printing in Biomedical Research
- Bone Tissue Engineering Materials
- Cancer Cells and Metastasis
- MicroRNA in disease regulation
- Nanoplatforms for cancer theranostics
- Cellular Mechanics and Interactions
- Metabolism, Diabetes, and Cancer
- Ferroptosis and cancer prognosis
- Orthopaedic implants and arthroplasty
- Orthopedic Infections and Treatments
- Bone and Joint Diseases
- Cell Adhesion Molecules Research
- Dental Implant Techniques and Outcomes
- Innovative Microfluidic and Catalytic Techniques Innovation
- Bone Metabolism and Diseases
- Cancer-related molecular mechanisms research
- Biomarkers in Disease Mechanisms
- Graphene and Nanomaterials Applications
- Mesenchymal stem cell research
Shanghai Ninth People's Hospital
2020-2025
Shanghai Jiao Tong University
2020-2025
Diabetic osteoporosis (DOP) is the leading complication continuously threatening bone health of patients with diabetes. A key pathogenic factor in DOP loss osteocyte viability. However, mechanism death remains unclear. Here, we identified ferroptosis, which iron-dependent programmed cell death, as a critical murine models DOP. The diabetic microenvironment significantly enhanced ferroptosis vitro, shown by substantial lipid peroxidation, iron overload, and aberrant activation pathway. RNA...
Abstract The specific mechanisms underlying bacteria‐triggered cell death and osteogenic dysfunction in host bone marrow mesenchymal stem cells (BMSCs) remain unclear, posing a significant challenge to the repair of infected defects. This study identifies ferroptosis as predominant cause BMSCs microenvironment. Mechanistically, bacteria‐induced activation innate immune response leads upregulation phosphorylation interferon regulatory factor 7 (IRF7), thus facilitating IRF7‐dependent through...
Abstract Antimicrobial resistance and impaired bone regeneration are the great challenges in treating infected defects. Its recurrent resistant nature, high incidence rate, long‐term hospitalization, medical costs have driven efforts of scientific community to develop new therapies improve situation. Considering complex microenvironment persistent mechanisms mediated by bacteria, it is crucial an implant with enhanced osseointegration sustained effective infection clearance effects. Here, a...
Abstract Although great progress has been made in engineered bone tissues, delayed or ineffective regeneration remains an issue due to the lack of neural network reconstruction their design. Therefore, tissue construct that mimics ossification center microenvironment promote innervation is proposed. Based on this, NGF@Lap constructs are constructed through bioprinting technology, which can release nerve growth factor (NGF) for a long time and simulate center's with high expression NGF. In...
Current in vitro models for osteosarcoma investigation and drug screening, including two-dimensional (2D) cell culture tumour spheroids (i.e. cancer stem-like cells), lack extracellular matrix (ECM). Therefore, results from traditional may not reflect real pathological processes genuine histological structures. Here, we report a three-dimensional (3D) bioprinted model (3DBPO) that contains cells shrouding ECM analogue 3D frame. Photo-crosslinkable bioinks composed of gelatine methacrylamide...
Osteocytes, essential regulators of bone homeostasis, are embedded in the mineralized matrix. Given spatial arrangement osteocytes, bioprinting represents an ideal method to biofabricate a 3D osteocyte network with suitable surrounding matrix similar native tissue. Here, we reported bioprinted osteocyte-laden hydrogel for biomimetic mineralization vitro exceptional shape fidelity, high cell density (107 cells per ml) and viability (85%-90%). The bioinks were composed modified biopolymers,...
To evaluate the interbody fusion efficacy and biocompatibility of a graft-free cage made polyetheretherketone/calcium silicate composite/porous tantalum (PEEK/CS/pTa cage) compared with PEEK/CS an autogenous bone graft in goat model. PEEK/CS/pTa cages were prepared through injection-moulding method. The composites porous characterized by Fourier transform infrared spectroscopy (FTIR), X-ray photoelectron (XPS), diffraction (XRD), scanning electron microscopy (SEM), energy-dispersive (EDS)...
Senile osteoporosis is characterized by age-related bone loss and microarchitecture deterioration. However, little known to date about the mechanism that maintains homeostasis during aging. In this study, we identify adenosine monophosphate-activated protein kinase alpha 1 (AMPKα1) as a critical factor regulating senescence lineage commitment of mesenchymal stem cells (MSCs). A phospho-mutant mouse model shows constitutive AMPKα1 activation prevents promoted MSC osteogenic with increased...