A5066992942- Neonatal Respiratory Health Research
- Transplantation: Methods and Outcomes
- Tissue Engineering and Regenerative Medicine
- Congenital Diaphragmatic Hernia Studies
- Lung Cancer Diagnosis and Treatment
- Pleural and Pulmonary Diseases
- Organ Transplantation Techniques and Outcomes
- Respiratory Support and Mechanisms
- Mechanical Circulatory Support Devices
- Lung Cancer Treatments and Mutations
- Cancer Cells and Metastasis
- Medical Imaging and Pathology Studies
- Tracheal and airway disorders
- Pulmonary Hypertension Research and Treatments
- Renal Transplantation Outcomes and Treatments
- Radiomics and Machine Learning in Medical Imaging
- Congenital heart defects research
- Cancer Immunotherapy and Biomarkers
- Esophageal and GI Pathology
- Angiogenesis and VEGF in Cancer
- Radiation Dose and Imaging
- Inhalation and Respiratory Drug Delivery
- Trauma Management and Diagnosis
- Organ Donation and Transplantation
- Cardiac Arrest and Resuscitation
Medical University of South Carolina
2018-2025
Brigham and Women's Hospital
2010-2023
Harvard University
2011-2023
Salisbury District Hospital
2023
University of Virginia
2022
Public Health England
2022
UK Health Security Agency
2022
Penn State Milton S. Hershey Medical Center
2013
Johannes Gutenberg University Mainz
2012
University of Florida
2003
In most rodents and some other mammals, the removal of one lung results in compensatory growth associated with dramatic angiogenesis complete restoration capacity. One pivotal mechanism neoalveolarization is neovascularization, because without new alveoli can not be formed. The aim this study to image analyze three-dimensionally different patterns neovascularization seen following pneumonectomy mice on a sub-micron-scale. C57/BL6 underwent left-sided pneumonectomy. Lungs were harvested at...
Objectives: The aim of this study was to summarize waitlist and transplant outcomes in kidney, liver, lung, heart transplantation using organ donation after circulatory death (DCD). Summary Background: DCD has expanded the donor pool for solid transplantation, most recently transplantation. Methods: United Network Organ Sharing registry used identify adult candidates recipients recent allocation policy eras transplant. Transplant were grouped by acceptance criteria versus brain-dead donors...
Abstract In most mammalian species, the removal of one lung results in dramatic compensatory growth remaining lung. To investigate contribution alveolar macrophages (AMs) to murine post‐pneumonectomy growth, we studied bronchoalveolar lavage (BAL)‐derived AM on 3, 7, 14 and 21 days after left pneumonectomy. BAL demonstrated a 3.0‐fold increase (CD45 + , CD11b − CD11c F4/80 Gr‐1 ) by Cell cycle flow cytometry BAL‐derived cells an S G2 phase 3 (11.3 ± 2.7%) 7 (12.1 1.8%) Correspondingly,...
Blood-borne nucleated cells participate not only in inflammation, but tissue repair and regeneration. Because progenitor stem cell populations have a low concentration the blood, circulation kinetics distribution of these is largely unknown. An important approach to tracking lineage use fluorescent tracers parabiotic models cross-circulation. Here, we investigated cross-circulation C57/B6 GFP(+)/wild-type parabionts. Flow cytometry analysis peripheral blood after parabiosis demonstrated no...
Abstract The oncotherapeutic promise of IL-15, a potent immunostimulant, is limited by short serum t1/2. fusion protein N-803 chimeric IL-15 superagonist that has >20-fold longer in vivo t1/2 versus IL-15. This phase 1 study characterized the pharmacokinetic (PK) profile and safety after s.c. administration to healthy human volunteers. Volunteers received two doses N-803, each dose, PK were assessed for 9 d. primary endpoint was profile, secondary safety, immune cell levels...
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Thoracic surgical procedures in mice have been applied to a wide range of investigations, but little is known about the murine physiologic response pulmonary surgery. Using continuous arterial oximetry monitoring and FlexiVent ventilator, authors investigated effect anesthesia pneumonectomy on mouse oxygen saturation lung mechanics. Sedation resulted dose-dependent decline that ranged from 55% 82%. Oxygen was restored by mechanical ventilation with increased rate tidal volumes. In strain...
The burden of imported rickettsial infection in the UK is not previously described. This retrospective review identifies cases diagnosed at national reference laboratory between 2015 and 2022.Samples testing positive for spotted fever group, typhus scrub IgG/IgM on acute convalescent blood samples, and/or PCR tissue/blood were categorized as suspected, confirmed or past infection.220 patients had rickettsioses, commonest import was group (61%, 125/205), 54% (62/114) from South Africa. In...
Respiratory muscle-associated stretch has been implicated in normal lung development (fetal breathing movements) and postpneumonectomy growth. To test the hypothesis that mechanical from diaphragmatic contraction contributes to growth, we performed left phrenic nerve transections (PNT) mice with without ipsilateral pneumonectomy. PNT was demonstrated by asymmetric costal margin excursion confirmed at autopsy. In two lungs, associated a decrease volume ( P < 0.05) weight 0.05). After...
Abstract To investigate the local mechanical forces associated with intravascular pillars and vessel pruning, we studied conducting vessels in extraembryonic circulation of chick embryo. During development days 13–17, blood flow parameters were identified using fluorescent vascular tracers digital time‐series video reconstructions. The geometry selected was confirmed by corrosion casting scanning electron microscopy. Computational simulations pruning suggested that serial form along...
Abstract Although blood vessel growth occurs readily in the systemic bronchial circulation, angiogenesis pulmonary circulation is rare. Compensatory lung after pneumonectomy an experimental model with presumed alveolar capillary angiogenesis. To investigate genes participating murine neoalveolarization, we studied expression of endothelial cells. After left pneumonectomy, remaining right was examined on days 3, 6, 14 and 21days surgery compared to both no sham thoracotomy controls. The lungs...
In many species, pneumonectomy results in compensatory growth the remaining lung.Although late mechanical consequences of murine are known, little is known about anatomic adaptations and respiratory mechanics during lung growth.To investigate structural changes growth, mice were studied for 21 days after left using microCT system impedance (FlexiVent).Anatomic included minimal mediastinal shift or chestwall remodeling, but significant displacement heart cardiac lobe.Mean lobe centroid was...
Abstract Background An intriguing biologic process in most adult mammals is post-pneumonectomy lung regeneration, that is, the removal of one (pneumonectomy) results rapid compensatory growth remaining lung. The spatial dependence and metabolic activity rodent during regeneration largely unknown. Methods To determine if murine could be detected vivo , we studied inbred mice 3, 7, 14, 21 days after left pneumonectomy. was imaged using microCT as well glucose tracer 2-deoxy-2-[ 18...
The intimate anatomic and functional relationship between epithelial cells endothelial within the alveolus suggests likelihood of a coordinated response during postpneumonectomy lung growth. To define population dynamics potential contribution alveolar to angiogenesis, we studied Type II I 21 days after pneumonectomy. Alveolar were defined isolated by flow cytometry using CD45 − , MHC class + phosphine phenotype. These phenotypically demonstrated an increase in cell number pneumonectomy;...
Objectives: The mesothelium, the surface layer of heart, lung, bowel, liver and tunica vaginalis, is a complex tissue implicated in organ-specific diseases regenerative biology; however, mechanism mesothelial repair after surgical injury unknown. Previous observations indicated seeding denuded mesothelium by free-floating cells may contribute to healing. In this study, we investigated prevalence pleural fluid during 7 days following pulmonary surgery. Study design: Flow cytometry was...
Complement is known to play a role in ischemia and reperfusion injury (IRI). A general paradigm that complement activated by self-reactive natural IgM antibodies (nAbs), after they engage postischemic neoepitopes. However, for nAbs lung transplantation (LTx) has not been explored. Using mouse models of LTx, we investigated the two neoepitopes, modified annexin IV (B4) subset phospholipids (C2), LTx. Antibody deficient Rag1-/- recipient mice were protected from LTx IRI. Reconstitution with...
In many mammalian species, the removal of one lung [pneumonectomy (PNX)] is associated with compensatory growth remaining lung. To investigate hypothesis that parenchymal deformation may trigger regeneration, we used respiratory-gated micro-computed tomography scanning to create three-dimensional finite-element geometric models murine cardiac lobe cyclic breathing. Models were constructed scans pre-PNX and 24 h post-PNX. The computational demonstrated maximum stretch ratio map was patchy...
In many mammalian species, the removal of one lung (pneumonectomy) is associated with compensatory growth remaining lung. To investigate hypothesis that parenchymal deformation may trigger regeneration, we used microCT scanning to create 3D finite element geometric models murine pre- and post-pneumonectomy (24 h). The structural correspondence between was established using anatomic landmarks an iterative computational algorithm. When compared pre-pneumonectomy lung, demonstrated significant...