A5005821123- Cardiac Fibrosis and Remodeling
- Liver Disease Diagnosis and Treatment
- Peptidase Inhibition and Analysis
- Radiomics and Machine Learning in Medical Imaging
- Nutritional Studies and Diet
- Lipoproteins and Cardiovascular Health
- Calpain Protease Function and Regulation
- Biliary and Gastrointestinal Fistulas
- Cell Adhesion Molecules Research
- Gastrointestinal Tumor Research and Treatment
- Protein Kinase Regulation and GTPase Signaling
- Cholangiocarcinoma and Gallbladder Cancer Studies
- Hepatitis B Virus Studies
- Intestinal and Peritoneal Adhesions
- Lanthanide and Transition Metal Complexes
- Phosphodiesterase function and regulation
- Cholesterol and Lipid Metabolism
- Bone and Joint Diseases
- Renin-Angiotensin System Studies
- Interstitial Lung Diseases and Idiopathic Pulmonary Fibrosis
- Health, Environment, Cognitive Aging
- Neutrophil, Myeloperoxidase and Oxidative Mechanisms
- Heparin-Induced Thrombocytopenia and Thrombosis
- Neurological and metabolic disorders
- Infectious Encephalopathies and Encephalitis
Sysmex (Japan)
2016
Kurume University
2010-2013
Funai Electric (Japan)
1993-2004
Yamagata University Hospital
1991
Hiroshima University
1983-1985
Kyoto Bunkyo University
1975
Kyoto University
1975
Kyoto University Research Reactor Institute
1975
Iron-containing and manganese-containing superoxide dismutases were found in Plectonemo boryanum.The Mn-enzyme occupies about 10% of total activity.The' Fe-enzyme was puriiied to near homogeneity.It contains 2 atoms iron per mol.Its molecular weight is 41,700 it composed two subunits identical without disultide linkage.Amino acid composition presented.Electron paramagnetic resonance spectrum revealed that occurs a high spin ferric form some anisotropic environment.The absorption the absence...
Twenty-seven gallbladder carcinomas were examined by computed tomography (CT). All patients had abnormal CT findings, suggesting a lesion in 26. Based on the major cancer was classified as "massive," "thickened wall," or "intraluminal." Twenty cases diagnosed correctly cancer: retrospect, definite findings of observed 24 and equivocal misleading signs 3. Some chronic cholecystitis liver tumor difficult to differentiate from cancer. Direct hepatic extension easily seen, but lymph-node...
More than 97% of spectrin phosphatase activity in human erythrocyte hemolysate was recovered cytosol.The cytosolic resolved into four peaks, namely phosphatases I (22% M, = 180,000), I1 (3%, 42,000), I11 (8%, 177,000), and IV (62%, 104,000), by aminohexyl-Sepharose preparatlan showed only 2 bands corresponding to band 1 Fairbanks a l .(231 stored at -80-C until use.C y C on an SDS-polyacrylamide gel electrophoresls the method nucleotides-l~aeoendent Drotern kinase Dartiallv Purlfled from h m...
Retinal neovascularization (NV) due to retinal ischemia remains one of the principal causes vision impairment in patients with ischemic diseases. We recently reported that periostin (POSTN) may play a role development preretinal fibrovascular membranes, but its NV has not been determined. The purpose this study was examine expression POSTN retinas mouse model oxygen-induced NV. also studied function on using Postn KO mice and human endothelial cells (HRECs) culture. In addition, we used...
Remnant-like lipoprotein particle cholesterol (RLP-C) is a highly atherogenic factor. RLP-C induces endothelial dysfunction and associated with hyperinsulinemia. This study was designed to determine whether high plasma levels predispose the development of hypertension in subjects normal blood pressure (BP).A total 1,485 aged >40 years Japanese Cohort Seven Countries Study received health examinations. We examined BP, anthropometric parameters, chemistries, including fasting levels. were...
Metastasis is the leading cause of cancer death. A tumor‐supportive microenvironment, or premetastatic niche, at potential secondary tumor sites plays an important role in metastasis, especially cell colonization. Although a fibrotic milieu known to promote tumorigenesis and underlying molecular contributors this effect have remained unclear. Here we show that periostin, component extracellular matrix functions tissue remodeling, has key formation environment promotes metastatic We found...
BackgroundEndothelin-1 (ET-1) is a potent vasoconstrictor derived from the endothelium. However, most large scale cross-sectional studies in humans have indicated no relationship between plasma ET-1 levels and hypertension. The present study was designed to determine whether high predict development of
Tubulin was phosphorylated mainly at tyrosine residues by membranes from mouse liver and Ehrlich ascites tumor with ATP in the presence of MnC12, ZnCl,, NaVO3, Nonidet P-40 an epidermal growth factor (EGF)-and insulin-independent manner.The tubulin kinase activity is comparable to membranes.Two kinases, namely I (Mr = 64,000) I1 46,000), were purified 5-17-fold free EGF receptor insulin receptor.Phosphorylation endogenous proteins produced major alkali-resistant phosphoproteins 56 53 kDa...
To study the role of periostin in adhesion formation, effect antisense oligonucleotide (PAO) on formation was evaluated mice. Under anesthesia, serous membrane cecum abraded, and score mRNA levels its related factors were determined after surgery. Saline, 40 mg/kg negative sense (NSO), or PAO injected into abdomen surgery, 14 days later. Filmy observed 1 day increased gradually to days. The periostin, transforming growth factor (TGF)-β, collagen I from 3 significantly lower than saline NSO...
When membrane fractions from mouse liver. Ehrlich ascites tumor and MH 134 hepatoma were incubated with [γ‐ 32 ]ATP at O°C in the presence of MnCly ZnCl; NaV0 3 , proteins phosphorylated on tyrosines to a larger extent liver membranes than membranes. Separation labelled by SDS‐gel electrophoresis showed alkali‐resistant bands 170, 140, 130, 80, 56, 53 46 kDa membranes; exhibited more strongly proteins. Epidermal growth factor stimulated tyrosine phosphorylation only 170 protein, which was...
<h3>Introduction</h3> Rapid socioeconomic development in Japan since beginning of the Seven Countries Study 1958 has brought remarkable changes lifestyle and dietary patterns. We investigated relationship between time trends nutrient intake serum cholesterol levels a Japanese cohort Study, Tanushimaru, typical farming town on Kyushu Island. <h3>Methods</h3> Subjects totalled 628 1958, 539 1977, 602 1982, 752 1989, 402 1999, 329 2009, all subjects were men aged 40–64 years. Eating patterns...
The dephosphorylated form of phosphorylase kinase was purified 700‐fold from rabbit heart extract. enzyme had a pH 6.8/pH 8.2 activity ratio 0.04–0.08 and completely dependent on Ca 2+ with an apparent K value for 2.59 μM at 6.8. At free concentrations between 0.057 400 μM, 1.5 troponin complex no significant effect the reaction. However, skeletal muscle stimulated reaction 1.5–2‐fold concomitant decrease in to 1.40 μM. No differences effects these complexes were observed when heart‐type...
<h3>Introduction</h3> Homocysteine has been implicated in promoting atherosclerotic vascular disease. We have already demonstrated that high level of plasma homocysteine is an independent correlates for increased carotid artery wall thickness cross-sectional study a same cohort. The present was designed to determine whether levels predict the development hypertension after ten years. <h3>Methods</h3> A periodic epidemiological survey performed 1999 Tanushimaru town. Data on fasting were...
We investigated the inhibitory effects of neopterin (NP) and its reduced form, 5,6,7,8-tetrahydroneopterin (NPH4), on carbon tetrachloride (CCl4)-induced hepatotoxicity. In in vivo experiments, intraperitoneal administration NP or NPH4 significantly inhibited elevation plasma alanine aminotransferase activity induced by CCl4 mice. vitro experiments using cultured rat hepatocytes, a manner which was both time- dose-dependent lactate dehydrogenase release, addition to culture-medium release...
The rate of dephosphorylation isolated P-H2B histone by pig heart phosphoprotein phosphatase (Mr = 224,000) and its catalytic subunit 31,000) was suppressed more than 95% at low ionic strength when the substrate integrated into nucleosome core particles. increased 13-43-fold polyamine hydrochlorides Mg(CH3COO)2 optimal 0.2-0.38 M. Phosphatase activity toward distributed in both rat liver cytosol nuclear extracts. activities these fractions showed similar specific were also 10-32-fold 10 mM...
Tsukagawa, E.; Adachi, H.; Hirai, Y.; Enomoto, M.; Fukami, A.; Otsuka, Miyamoto, T.; Nanjo, Yoshikawa, K.; Kumagai, Esaki, Ogata, Yokoi, Murayama, Imaizumi, T. Author Information