A5025974014- Cancer Genomics and Diagnostics
- Genetic factors in colorectal cancer
- Ovarian cancer diagnosis and treatment
- BRCA gene mutations in cancer
- Endometrial and Cervical Cancer Treatments
- Epigenetics and DNA Methylation
- Genomics and Rare Diseases
- Ferroptosis and cancer prognosis
- PARP inhibition in cancer therapy
- Colorectal and Anal Carcinomas
- Acute Myeloid Leukemia Research
- Cholangiocarcinoma and Gallbladder Cancer Studies
- Cervical Cancer and HPV Research
- Cancer-related molecular mechanisms research
- Molecular Biology Techniques and Applications
- Chromatin Remodeling and Cancer
- CRISPR and Genetic Engineering
- Neonatal Respiratory Health Research
- Medical Imaging Techniques and Applications
- Histone Deacetylase Inhibitors Research
- Pancreatic and Hepatic Oncology Research
- Viral-associated cancers and disorders
- Cancer Treatment and Pharmacology
- Radiation Dose and Imaging
- DNA Repair Mechanisms
Agostino Gemelli University Polyclinic
2021-2025
Istituti di Ricovero e Cura a Carattere Scientifico
2021-2025
Università Cattolica del Sacro Cuore
2024
Science & Technology Park (Czechia)
2024
Institute for Systems Analysis
2024
National Academies of Sciences, Engineering, and Medicine
2024
ORCID
2023
European Institute of Oncology
2010-2019
University of Rome Tor Vergata
2012
The identification of genes maintaining cancer growth is critical to our understanding tumorigenesis. We report the first in vivo genetic screen patient-derived tumors, using metastatic melanomas and targeting 236 chromatin by expression specific shRNA libraries. Our screens revealed unprecedented numerosity indispensable for tumor (∼50% tested genes) unexpected functional heterogeneity among patients (<15% common). Notably, these were not activated somatic mutations same are therefore...
The E3 ubiquitin ligase (E3) WWP1 is an oncogenic factor implicated in the maintenance of different types epithelial cancers. role WW domain-containing protein 1 (WWP1) haematological neoplasms remains unknown. Acute myeloid leukaemia (AML) characterized by expansion malignant cells blocked at stages differentiation. Here we report that expression significantly augmented a large cohort primary AML patients and cell lines, compared with haematopoietic from healthy donors. We show inactivation...
Abstract Background Extremely low gestational age neonates (ELGANs, i.e., born before 28 weeks of gestation) are at high risk developing retinopathy prematurity (ROP), with potential long-life visual impairment. Due to concomitant anemia, ELGANs need repeated red blood cell (RBC) transfusions. These produce a progressive replacement fetal hemoglobin (HbF) by adult (HbA). Furthermore, close association exists between levels HbF and severe ROP, suggesting that perturbation the HbF-mediated...
Abstract Background High-grade serous ovarian cancer (HGSOC) has poor survival rates due to a combination of diagnosis at advanced stage and disease recurrence as result chemotherapy resistance. In BRCA1 (Breast Cancer gene 1) - or BRCA2-wild type (BRCAwt) HGSOC patients, resistance progressive occur earlier more often than in mutated BRCA. Identification biomarkers helpful predicting response first-line is challenge improve BRCAwt management. Methods To identify signature that can predict...
Abstract Background To date, 11 DNA polymerase epsilon ( POLE ) pathogenic variants have been declared “hotspot” mutations. Patients with endometrial cancer (EC) characterized by hotspot mutations mut) exceptional survival outcomes. Whereas international guidelines encourage deescalation of adjuvant treatment in early‐stage mut EC, data regarding safety patients unfavorable characteristics are still under investigation. On the other hand, spread comprehensive genome profiling programs has...
771 Background: Mutations (muts) in oncosuppressor genes have been associated with poor prognosis different solid tumors. This study aims to evaluate the prognostic impact of p53 pathway muts mPDAC pts. Methods: For each pt enrolled this a FFPE tumor tissue specimen was collected and Next Generation Sequencing (NGS) performed by TSO500HT assay (DNA [523 genes], RNA [55 genes]). Primary endpoint overall survival (OS). The Kaplan–Meier method used estimate efficacy outcome; log-rank test,...
Background/Objectives: The accurate prediction of adverse drug reactions (ADRs) to oncological treatments still poses a clinical challenge. Chemotherapy is usually selected based on trials that do not consider patient variability in ADR risk. Consequently, many patients undergo multiple find the appropriate medication or dosage, enhancing risks and increasing chance discontinuing therapy. We first aimed develop pharmacogenetic model for predicting chemotherapy-induced ADRs cancer (the...
The study's aim was to investigate the immunohistochemical (IHC) expression of biological markers as potential prognostic/therapeutic factors in vulvar squamous cell carcinoma (VSCC).A series 101 patients surgically treated at our center from 2016 2020 were retrospectively enrolled: 53 node-negative (Group A) and 48 node-positive B). A total 146 samples, primary tumor (T) 45 nodal metastases (N), investigated. IHC panel included: p16, p53, MLH1, MSH2, MSH6, PMS2, PD-L1, CD3, HER2/neu, ER,...
Ultrasound examination is an accurate method in the preoperative evaluation of inguinofemoral lymph nodes when performed by experienced operators. The purpose study was to build a robust, multi-modular model based on machine learning discriminate between metastatic and non-metastatic inguinal patients with vulvar cancer. One hundred twenty-seven women were selected at our center from March 2017 April 2020, 237 regions analyzed (75 162 histology). before surgery examiners. features defined...
The implementation of cancer molecular characterization in clinical practice has improved prognostic re-definition, extending the eligibility to a continuously increasing number targeted treatments. Broad profiling technologies better than organ-based approaches are believed serve such dynamic purposes. We here present workflow our institution adopted run comprehensive genome practice. This article describes designed make program feasible and sustainable large-volume referral hospital.
Complete response prediction in locally advanced rectal cancer (LARC) patients is generally focused on the radiomics analysis of staging MRI. Until now, omics information extracted from gut microbiota and circulating tumor DNA (ctDNA) have not been integrated composite biomarkers-based models, thereby omitting valuable decision-making process. In this study, we aim to integrate with ctDNA-based genomics tracking during neoadjuvant chemoradiotherapy (nCRT).
Medulloblastoma (MB) is the most common cerebellar malignancy during childhood. Among MB, MYC-amplified Group 3 tumors display worst prognosis. MYC an oncogenic transcription factor currently thought to be undruggable. Nevertheless, targeting MYC-dependent processes (i.e. and RNA processing regulation) represents a promising approach. We have tested sensitivity of MYC-driven MB cells pool splicing inhibitors that wide spectrum targets. them, we focus on THZ531, inhibitor transcriptional...
The Saccharomyces cerevisiae kinase/adenosine triphosphatase Rio1 regulates rDNA transcription and segregation, pre-rRNA processing small ribosomal subunit maturation. Other roles are unknown. When overexpressed, human ortholog RIOK1 drives tumor growth metastasis. Likewise, promotes 40S biogenesis has not been characterized globally. We show that manages directly via a series of regulators, an essential signaling network at the protein, chromatin RNA levels. orchestrates division depending...