A5040758810- Acute Myeloid Leukemia Research
- Epigenetics and DNA Methylation
- Pluripotent Stem Cells Research
- Insect Resistance and Genetics
- Studies on Chitinases and Chitosanases
- Insect symbiosis and bacterial influences
- Cancer Genomics and Diagnostics
- Spinal Fractures and Fixation Techniques
- Hematopoietic Stem Cell Transplantation
- RNA modifications and cancer
- Scoliosis diagnosis and treatment
- Genomics and Chromatin Dynamics
- Immune cells in cancer
- Cancer, Hypoxia, and Metabolism
- Spine and Intervertebral Disc Pathology
- CRISPR and Genetic Engineering
- Congenital heart defects research
- Metabolism and Genetic Disorders
- Histone Deacetylase Inhibitors Research
- Surgical site infection prevention
- MicroRNA in disease regulation
- Neuroinflammation and Neurodegeneration Mechanisms
- Immune Cell Function and Interaction
- Fungal Infections and Studies
- Retinal Diseases and Treatments
Air Force Medical University
2014-2024
Shaanxi Normal University
2022-2024
Shanghai Changzheng Hospital
2016-2024
Hangzhou Medical College
2024
Tongji University
2020-2024
Xijing Hospital
2021-2024
Shandong Provincial QianFoShan Hospital
2024
Shandong First Medical University
2024
Zhengzhou University
2024
Ministry of Agriculture and Rural Affairs
2022-2024
Differentiated somatic cells can be reprogrammed into totipotent embryos through cell nuclear transfer. However, most cloned arrest at early stages and the underlying molecular mechanism remains largely unexplored. Here, we first developed a transfer embryo biopsy system two- or four-cell stage, which allows us to trace developmental fate of biopsied precisely. Then, single-cell transcriptome sequencing with different fates, identified that inactivation Kdm4b, histone H3 lysine 9...
Solar vapor generation technology is promising in seawater desalination, sewage purification, and other fields. However, wide application of this still largely confined due to its high cost difficulties for scalable production. In study, an ever-floating solar evaporator fabricated by coating multiwall carbon nanotubes on a bicomponent nonwoven composed polypropylene/polyethylene core-sheath fibers. This all-fiber structure highly porous ultralight, with large specific area (for efficient...
Severe myoclonic epilepsy of infancy (SMEI, also known as Dravet syndrome) and genetic with febrile seizures plus (mild seizures) can both arise due to mutations SCN1A, the gene encoding alpha 1 pore-forming subunit Nav1.1 voltage-gated sodium channel. Owing inaccessibility patient brain neurons, precise mechanism mild SMEI remains elusive, there is no effective pharmacotherapy. Induced pluripotent stem cells (iPSCs) induced neurons (iNs) have been successfully generated from patients...
Abstract TET2 is a dioxygenase that catalyses multiple steps of 5-methylcytosine oxidation. Although mutations frequently occur in various types haematological malignancies, the mechanism by which they increase risk for these cancers remains poorly understood. Here we show Tet2 −/− mice develop spontaneous myeloid, T- and B-cell malignancies after long latencies. Exome sequencing tumours reveals accumulation numerous mutations, including Apc , Nf1 Flt3 Cbl Notch1 Mll2 are recurrently...
Abstract Clonal hematopoiesis of indeterminate potential (CHIP) increases with age and is associated increased risks hematological malignancies. While TP53 mutations have been identified in CHIP, the molecular mechanisms by which mutant p53 promotes hematopoietic stem progenitor cell (HSPC) expansion are largely unknown. Here we discover that confers a competitive advantage to HSPCs following transplantation HSPC after radiation-induced stress. Mechanistically, interacts EZH2 enhances its...
Generation of widely differing and specialized cell types from a single totipotent zygote involves large-scale transcriptional changes chromatin reorganization. Pioneer transcription factors play key roles in programming the epigenome facilitating recruitment additional regulatory during successive lineage specification differentiation steps. Here we show that Isl1 acts as pioneer factor driving cardiomyocyte commitment by shaping landscape cardiac progenitor cells. Using an hypomorphic...
Abstract Blood vessels play a role in osteogenesis and osteoporosis; however, the of vascular metabolism these processes remains unclear. The present study finds that ovariectomized mice exhibit reduced blood vessel density bone expression endothelial glycolytic regulator pyruvate kinase M2 (PKM2). Endothelial cell (EC)‐specific deletion Pkm2 impairs worsens osteoporosis mice. This is attributed to impaired ability mesenchymal stem cells (BMSCs) differentiate into osteoblasts....
Improving the mechanical properties of ionogels is crucial for their widespread practical application.
The problem of mobile phone addiction and academic procrastination among medical students has been widely acknowledged. This study aimed to explore the influence demographic factors on addiction, procrastination, achievement students. Further, it investigated association between achievement. cross-sectional was conducted May June 2019. A total 3 511 participated in an online questionnaire survey (effective response rate = 81.7%). Demographic factors, Scale Academic Achievement, short scale...
Abstract The limited and unstable absorption of excess exudate is a major challenge during the healing infected wounds. In this study, highly stable, multifunctional Janus dressing with unidirectional transfer capacity fabricated based on single poly(lactide caprolactone) (PLCL). success method relies an acid hydrolysis reaction that transforms PLCL fibers from hydrophobic to hydrophilic in situ. resulting interfacial affinity between hydrophilic/phobic endows structure excellent liquid high...
The creation of a functional 3D bioprinted human heart remains challenging, largely due to the lack some crucial cardiac cell types, including atrioventricular canal (AVC) cardiomyocytes, which are essential slow down electrical impulse between atrium and ventricle. By utilizing single-cell RNA sequencing analysis bioprinting technology, we discover that stage-specific activation canonical Wnt signaling creates AVC cardiomyocytes derived from pluripotent stem cells. These display...
Polycomb group (PcG) proteins function as two major multicomponent protein complexes: repressive complex 1 (PRC1) and 2 (PRC2) to repress key developmental genes maintain epigenetic memory of cell identity during development. Phase-separation has long been implicated in PRC1 mediated gene silencing, but it is still unclear whether PRC2 also utilize a similar mechanism regulate expression. Here we report that Polycomb-like (PCL) proteins, accessory factors, can phase-separate vitro generate...
Objective To investigate the effect of calcium channel blockers (CCBs) on tacrolimus blood concentrations in renal transplant recipients with different CYP3A5 genotypes. Methods This retrospective cohort study included receiving tacrolimus-based immunosuppressive therapy or without CCBs combination. Patients were divided into combination and control groups based whether not they combined CCBs, then further analyzed according to type (nifedipine/amlodipine/felodipine). Propensity score...
The application of a maxillofacial prosthesis is an alternative to surgery in functional-aesthetic facial reconstruction. Computer aided design/computer manufacturing has opened up new approach the fabrication prostheses. An intelligentized rapid simulative design and system for prostheses was developed facilitate procedure.The simulation consists three components: digital impression, design, manufacturing. patients' impressions were taken with structured-light 3D scanner; then, model their...
Most neurodegenerative diseases are late-onset and aging-related, no effective treatments have been developed. The successful generation of induced pluripotent stem cells (iPSCs) the direct conversion neurons from patients' specific somatic offered cell resources for disease modeling potential transplantation therapy. However, to date, systematic studies investigated which approach is more suitable future In this study, using two approaches mentioned above in parallel we successfully...
Loss-of-function mutations in cerebral cavernous malformation (CCM) genes and gain-of-function mutation the MAP3K3 gene encoding MEKK3 cause CCM. Deficiency of CCM proteins leads to activation MEKK3-KLF2/4 signaling, but it is not clear how this occurs. Here, we demonstrate that deletion CCM3 interacting kinases STK24/25 endothelial cells causes defects vascular patterning during development as well lesion formation postnatal life. While permanent caused developmental system, inducible...