Daniel J. Phillips
A5000717053- SARS-CoV-2 and COVID-19 Research
- Advanced Polymer Synthesis and Characterization
- COVID-19 Clinical Research Studies
- Polymer Surface Interaction Studies
- Nanoparticle-Based Drug Delivery
- Acute Ischemic Stroke Management
- Hydrogels: synthesis, properties, applications
- biodegradable polymer synthesis and properties
- SARS-CoV-2 detection and testing
- Plant Diversity and Evolution
- Plant and animal studies
- Ecology and Vegetation Dynamics Studies
- Immunotherapy and Immune Responses
- Viral Infections and Immunology Research
- Trauma and Emergency Care Studies
- Thermodynamic properties of mixtures
- Chemical and Physical Properties in Aqueous Solutions
- Drug Solubulity and Delivery Systems
- RNA Research and Splicing
- Molecular Sensors and Ion Detection
- Advanced biosensing and bioanalysis techniques
- Long-Term Effects of COVID-19
- Analytical Chemistry and Chromatography
- Crystallography and molecular interactions
- Surfactants and Colloidal Systems
JPS Health Network
2024
Oxford BioMedica (United Kingdom)
2024
University of Oxford
2020-2024
East of England Ambulance Service NHS Trust
2020-2024
Churchill Hospital
2022
Oxford Centre for Diabetes, Endocrinology and Metabolism
2022
Institut de Recherche Vaccinale
2021
Saarland University
2021
Jenner Institute
2020
Cardiff University
2019
The ChAdOx1 nCoV-19 (AZD1222) vaccine has been approved for emergency use by the UK regulatory authority, Medicines and Healthcare products Regulatory Agency, with a regimen of two standard doses given an interval 4-12 weeks. planned roll-out in will involve vaccinating people high-risk categories their first dose immediately, delivering second 12 weeks later. Here, we provide both further prespecified pooled analysis trials exploratory analyses impact on immunogenicity efficacy extending...
Abstract The global supply of COVID-19 vaccines remains limited. An understanding the immune response that is predictive protection could facilitate rapid licensure new vaccines. Data from a randomized efficacy trial ChAdOx1 nCoV-19 (AZD1222) vaccine in United Kingdom was analyzed to determine antibody levels associated with against SARS-CoV-2. Binding and neutralizing antibodies at 28 days after second dose were measured infected noninfected recipients. Higher all markers correlated reduced...
<h2>Summary</h2><h3>Background</h3> A new variant of SARS-CoV-2, B.1.1.7, emerged as the dominant cause COVID-19 disease in UK from November, 2020. We report a post-hoc analysis efficacy adenoviral vector vaccine, ChAdOx1 nCoV-19 (AZD1222), against this variant. <h3>Methods</h3> Volunteers (aged ≥18 years) who were enrolled phase 2/3 vaccine studies UK, and randomly assigned (1:1) to receive or meningococcal conjugate control (MenACWY) provided upper airway swabs on weekly basis also if they...
Abstract Background Although 6 COVID-19 vaccines have been approved by the World Health Organisation as of 16 th June 2021, global supply remains limited. An understanding immune response associated with protection could facilitate rapid licensure new vaccines. Methods Data from a randomised efficacy trial ChAdOx1 nCoV-19 (AZD1222) vaccine in UK was analysed to determine antibody levels against SARS-CoV-2. Anti-spike and anti-RBD IgG multiplex immunoassay, pseudovirus live neutralising at 28...
Abstract The immune mechanisms mediating COVID-19 vaccine attenuation of remain undescribed. We conducted comprehensive analyses detailing responses to SARS-CoV-2 virus in blood post-vaccination with ChAdOx1 nCoV-19 or a placebo. Samples from randomised placebo-controlled trials (NCT04324606 and NCT04400838) were taken at baseline, onset COVID-19-like symptoms, 7 days later, confirming using nucleic amplification test (NAAT test) via real-time PCR (RT-PCR). Serum cytokines measured...
The potential to fine-tune the transition temperatures of polymers displaying lower-critical solution (LCST) by a simple mixing strategy is investigated. Using panel four distinct polymer classes (poly[oligo(ethyleneglycol)methacrylate], poly(N-vinylpiperidone), poly(N-vinylcaprolactam) poly(N-isopropylacrylamide)) it was shown that only those with strong molecular weight dependent LCSTs produced single, cooperative, when blended together. Furthermore, actual temperature linked average not...
Antimicrobial resistance is a global healthcare problem with dwindling arsenal of usable drugs. Tuberculosis, caused by Mycobacterium tuberculosis, requires long-term combination therapy and multi- totally drug resistant strains have emerged. This study reports the antibacterial activity cationic polymers against mycobacteria, which are distinguished from other Gram-positive bacteria their unique cell wall comprising covalently linked mycolic acid–arabinogalactan–peptidoglycan complex...
Disulfide linkages were introduced into poly (N-isopropylacrylamide) by the polycondensation of a RAFT-derived, telechelic macromonomer to give degradable yet vinyl-based polymers. These polymers displayed redox-sensitive lower critical solution temperature (LCST) with shorter, degraded product displaying higher LCST than its non-degraded counterpart.
Background: A new variant of SARS-CoV-2, B.1.1.7, emerged as the dominant cause COVID-19infection in United Kingdom from November 2020 with a transmission advantage over previous variants virus. Here we report efficacy adenoviral vector vaccine, ChAdOx1 nCoV-19, against this comparison non-B.1.1.7 lineages.Methods: Volunteers enrolled phase II/III vaccine studies and randomised 1:1 to receive nCoV-19 or MenACWY control providedupper airway swabs every week during trial also if they developed...
Abstract Several COVID-19 vaccines have shown good efficacy in clinical trials, but there remains uncertainty about the of against different variants. Here, we investigate ChAdOx1 nCoV-19 (AZD1222) symptomatic a post-hoc exploratory analysis Phase 3 randomised trial Brazil (trial registration ISRCTN89951424). Nose and throat swabs were tested by PCR participants. Sequencing genotyping performed to determine lineages SARS-CoV-2 circulating during study. Protection any caused Zeta (P.2)...
Mechanisms governing regional human adipose tissue (AT) development remain undefined. Here, we show that the long non-coding RNA HOTAIR (HOX transcript antisense RNA) is exclusively expressed in gluteofemoral AT, where it essential for adipocyte development. We find interacts with polycomb repressive complex 2 (PRC2) and identify core HOTAIR-PRC2 target genes involved lineage determination. Repression of coincides PRC2 promoter occupancy H3K27 trimethylation. also modifying gluteal...
Telechelic, RAFT (reversible addition–fragmentation chain transfer)-derived macromonomers with a pyridyl disulfide end-group were converted into high molecular weight, disulfide-linked polymers using polycondensation, step-growth procedure. The applicability of the method to polycondense library different functionalities including (meth)acrylates and acrylamides was investigated. Side-chain sterics found be important as nonlinear poly(ethylene glycol) analogues, which proved incompatible...
Abstract Tethering polymers to surfaces represents the cornerstone of a wide range applications, including stabilization colloids/biomolecules and preparation functional coatings. Unfortunately, despite prevalence protein‐tethered in pharmaceutical sector, analysis such polymer monolayers on molecular level is difficult. In this work, simple 1 H NMR spectroscopy catalytic properties α‐chymotrypsin are used analyze conformational/permeability protein‐bound poly(oligoethyleneglycol...
Thermo-responsive polymers are of broad interest in a range biomedical and biotechnological fields. This review summaries the use ‘isothermal’ transitions where thermo-responsive re-programmed to respond other stimuli, but with same outputs, aim making them ‘smarter’.
Graphene oxide is functionalized by grafting poly(<italic>N</italic>-isopropylacrylamide) across its surface in a single reaction step to give composite material with enhanced thermal stability and solubility.
Background: The ChAdOx1 nCoV-19 (AZD1222) vaccine has been approved for emergency use by the UK regulatory authority, MHRA, with a regimen of two standard doses given an interval between 4 and 12 weeks. planned rollout in will involve vaccinating people high risk categories their first dose immediately, delivering second weeks later.Here we provide both further prespecified pooled analysis trials exploratory analyses impact on immunogenicity efficacy extending priming booster doses. In...
External control over the antifreeze protein mimetic activity of poly(vinyl alcohol) is enabled by Fe<sup>3+</sup> driven supramolecular assembly.
Nanoparticles obtained by the nanoprecipitation of responsive polymers are shown to specifically and rapidly disassemble into hydrophilic polymer chains in presence intracellular concentrations glutathione.
The α-termini of RAFT-derived thermoresponsive polymers were functionalised via thiol exchange with pyridyl disulfide. Addition hydrophilic end-groups dramatically increased the observed cloud point polymers, without changing composition main chain. Selective cleavage disulfide-linked was under conditions intended to mimic intracellular glutathione concentration. This allowed behaviour be 'switched on' need for a temperature stimulus - an 'isothermal' switch.
The potential of a biphasic dissolution system to assist with the analysis controlled-release (CR), Biopharmaceutics Classification System (BCS) Class II pharmaceutical products has been investigated.Use medium (aqueous/octanol) provided sink conditions and afforded complete nifedipine formulated in CR matrix tablet while maintaining dosage form an aqueous environment.This was not possible monophasic (aqueousonly) medium.Consequently, model allowed discrimination three formulations...
Thermoresponsive polymers have attracted huge interest as a way of developing smart/adaptable materials for biomedicine, particularly due to changes in their solubility above the LCST. However, temperature is not always an appropriate or desirable stimulus given variety other cellular microenvironments that exist, including pH, redox potentials, ionic strength, and metal ion concentration. Here, we achieve highly specific, isothermal switch poly(N-isopropylacrylamide) by application ferric...
Au nanoparticles modified with catechol-functionalised polymers provide a strong optical response which is highly specific to Fe<sup>3+</sup> ions at physiologically relevant concentrations.
Multi-block copolymers offer a plethora of exciting properties, easily tuned by modulating parameters such as monomer composition, block length, number and dispersity.