The mycotoxin deoxynivalenol (DON) contaminates animal feed worldwide, frequently resulting in poor performance and economic losses. Data concerning the effects on poultry health or focusing intestinal toxicity response to oxidative stress are scarce. Also, there is a need for strategies mitigate negative of DON. This study aimed investigate Lactobacillus spp. treatments intestine, liver kidney fed DON-contaminated diet. To achieve this aim, histological, morphometrical histochemical assays...

Deoxynivalenol (DON) is a predisposing factor for necrotic enteritis. This study aimed to investigate the effects of DON and Clostridium perfringens (CP) challenge on intestinal morphology, morphometry, oxidative stress, immune response broilers. Additionally, we evaluated potential Lactobacillus spp. mixture as an approach mitigate damage induced by challenge. One-day-old broiler chickens (n = 252) were divided into seven treatment groups: Control, DON, CP, CP + VL (DON viable mixture), HIL...

The extensive use of antimicrobials in broiler production is changing the bird microbiota, fostering drug-resistant bacteria, and complicating therapeutic interventions, making problem multidrug resistance global. monitoring antimicrobial virulence genes are tools that have come to assist breeding these animals, directing possible treatments as already used human medicine collecting data demonstrate dissemination multidrug-resistant strains may cause damage industry public health. This work...

Galactomannans are abundant nonstarch polysaccharides in broiler feed ingredients. In broilers, diets with high levels of galactomannans have been associated innate immune response stimulation, poor zootechnical performance, nutrient and lipid absorption, excessive digesta viscosity. However, data about its effects on the gut microbiome scarce. β-Mannanases enzymes that can hydrolyze β-mannans, resulting better utilization. current study, we evaluated effect guar gum, a source...

This study aimed to characterize the in vitro and vivo adhesion immunomodulatory effect of Lactobacillus strains isolated from chickens. samples 65-wk-old birds were identified by PCR; their was evaluated via basement membrane-type cell matrix through carboxyfluorescein succinimidyl amino ester staining inoculation 1-d-old duodenum, jejunum, ileum, cecum collections at 1, 4, 12, 24 h after inoculation. The 5 best adhesive test formed a pool for total IgA IgG measurement sera intestinal...

Salmonella food poisoning is a public health problem. Feed withdrawal from broiler chickens before slaughter can favor the multiplication of in cecum and crop contaminated animals subsequently lead to contamination carcasses processing plant. In present study, cocktail lytic bacteriophages isolated sewage water was orally administered 45-d-old 1 h after they received an oral dose 10(7) cfu/mL enterica subspecies serotype Enteritidis. Immediately phage administration 30 min, 1, 3, 6, 12...

Salmonella spp. is one of the main agents responsible for foodborne infection in humans, and products poultry origin are most common sources. Studies have shown occurrence antimicrobials resistant animal products. The Extended Spectrum ?-Lactamase (ESBL) enzymes that confer to bacteria ability hydrolyze cephalosporin with an oximino side chain monobactams. This study aimed investigate antimicrobial resistance profile, identify phenotypes genotypes multiple drug (MDR) produce ESBL from...

Extraintestinal pathogenic

Considering the worrying emergence of multidrug resistance, including in animal husbandry and especially food-producing animals, need to detect antimicrobial resistance strains poultry environments is relevant, mainly considering a One Health approach. Thus, this study aimed conduct longitudinal monitoring broiler chicken farms, with an emphasis on evaluating frequency fosfomycin β-lactams. Escherichia coli was isolated from farms (cloacal swabs, meconium, feed, water, litter, Alphitobius...

Escherichia coli is a key indicator of food hygiene, and its monitoring in meat samples points to the potential presence antimicrobial-resistant strains capable causing infections humans, encompassing resistance profiles categorized as serious threats by Centers for Disease Control Prevention (CDC), such Extended-Spectrum Beta-Lactamase (ESBL)—a problem with consequences animal, human, environmental health. The objective present work was isolate characterize ESBL-producing E. from poultry,...

Ochratoxin A (OTA) is a mycotoxin produced by species of Penicillium and Aspergillus that can contaminate products plant origin are used as animal feed. Through oral exposure, this primarily affects the chicken gastrointestinal system. The present study evaluated intestinal toxic effects OTA introduction L-tryptophan to alleviate these in chickens. One-day-old chicks were exposed single dose (1.4 mg/kg body weight—b.w.) treated with or without four daily doses (100 b.w.). Duodenal villus...

Clostridium perfringens is the etiological agent of NE, a disease that greatly affects poultry industry. Experiments on induction NE are difficult to carry out, as it multifactorial disease, and thus different predisposing factors have been used. This study evaluated effect Gumboro vaccine virus (IBDV-vac) associated or not with infection by Eimeria spp. in broilers, factor for NE. Broilers (n = 99) were divided into groups (11) challenged IBDV-vac, CP type G (CP13, CP14 CP03) both. The...

This study investigates the effects of different doses serotonin, its precursor 5-hydroxytry-ptophan (5HTP), and m-hydroxybenzylhydrazine inhibitor (NSD1015), administered via intraperitoneal for 5 consecutive days, on behavior average body weight broilers. We also measured humoral immune response quantification Salmonella Enteritidis in broilers chickens that received drugs evaluated a Lactobacillus pool. The was divided into 3 experiments: Experiment 1--administration pharmaceuticals with...

In the aviculture industry, use of Lactobacillus spp. as a probiotic has been shown to be frequent and satisfactory, both in improving bird production indexes protecting intestine against colonization by pathogenic bacteria. Adhesion is an important characteristic selecting strains since it impedes its immediate elimination enable beneficial action host. This study aimed isolate, identify characterize vitro vivo adhesion isolated from birds. The was identified PCR sequencing evaluated via...

Due to the constant evolution of industrial poultry production and global emergence bacterial resistance antibiotics there has been an increasing interest in alternatives for treatment salmonellosis, such as phage therapy probiotics. The present study evaluated effects oral administration bacteriophage P22 a probiotic, consisting four Lactobacillus species, on level circulating heterophils containing superoxide anion one-day-old broilers challenged with Salmonella Typhimurium seven days. It...

This study investigated the immune response of broiler chickens with oral treatment a Lactobacillus spp. pool (PL) associated microencapsulated recombinant proteins flagellin (FliC) and subunit B cholera toxin (CTB). Immune responses were evaluated by measuring IgA from intestinal fluid, serum IgY, immunostaining CD8+ T lymphocytes present in cecum. The evaluations performed on d 0, 7, 14, 21, 28 posttreatment. A significant increase (P < 0.05) was observed levels all immunized groups,...

The pathological and molecular findings associated with Histomonas meleagridis are described in a leucistic Indian peafowl (Pavo cristatus) from Southern Brazil. most significant gross were multifocal necrotizing hepatitis diphtheric typhlitis. Histopathologic evaluation of the liver, ceca, kidney, spleen, small intestine revealed systemic histomoniasis (SH) intralesional intravascular accumulations histomonad organisms consistent H. meleagridis. PCR was used to amplify DNA intestine, lungs,...

Avian cellulitis causes significant losses to the poultry industry. Avian-pathogenic Escherichia coli (APEC) is etiological agent of that disease. This microorganism has zoonotic potential and may act as reservoir antimicrobial-resistance genes. In this context, production extended-spectrum B-lactamase (ESBL) one main antimicrobial resistance mechanisms. The objective study was determine ESBL in an (E. coli) strain isolated from avian lesions. Twenty-two E. Coli isolates were harvested...

After the growth-promoting antibiotics prohibition, intestinal health became an increasing concern worldwide in poultry farming. The histological evaluation is inexpensive technique that brings relevant information, but poultry, immediate process of post-mortem autolysis interferes directly on samples quality for analysis hindering a precise diagnosis. This study aimed to standardize broilers’ intestines sample collection and fixation analysis. Seven broiler chickens received standard diet...

A proteína Iss (increased serum survival) é uma importante característica de resistência ao sistema complemento da Escherichia coli patogênica para aves (APEC). Os objetivos deste trabalho foram clonar e verificar a diversidade seqüência do gene iss APEC caracterizar recombinante. O 309 bp foi amplificado por PCR, clonado expresso na E. BL21 (DE3) utilizando o vetor pET SUMO. APEC9 classificado como tipo 1 pela diferenciação entre 3 tipos alelos. expressa indução com IPTG, purificada em...

ABSTRACT: Enterococcal spondylitis affects poultry and causes progressive lameness. This study reports what seems to be the first case of vertebral osteomyelitis caused by Enterococcus in broiler chickens southern Brazil. We also conducted an experimental infection evaluate microorganismal characteristics pathogenicity chickens. performed bacterial isolation, identification, histopathology. The isolates were tested for their growth survival capacity at different temperatures, pH values,...