A5066667318- Animal Nutrition and Physiology
- Probiotics and Fermented Foods
- Gut microbiota and health
- Clostridium difficile and Clostridium perfringens research
- Salmonella and Campylobacter epidemiology
- Microbial Metabolites in Food Biotechnology
- Viral gastroenteritis research and epidemiology
- Meat and Animal Product Quality
- Rabbits: Nutrition, Reproduction, Health
- Genomics and Phylogenetic Studies
- Livestock and Poultry Management
- Food composition and properties
- Microbial Metabolism and Applications
- Antifungal resistance and susceptibility
- Microbial infections and disease research
- Bacteriophages and microbial interactions
- Mycotoxins in Agriculture and Food
- Biochemical Analysis and Sensing Techniques
- Inflammatory Bowel Disease
- Coccidia and coccidiosis research
- Bacterial biofilms and quorum sensing
- Bacterial Genetics and Biotechnology
- Streptococcal Infections and Treatments
- Animal Behavior and Welfare Studies
- Antimicrobial Resistance in Staphylococcus
Ghent University
2015-2025
Ghent University Hospital
2008-2023
Koninklijke Nederlandse Maatschappij voor Diergeneeskunde
2017
University of Groningen
2014
<h3>Objective</h3> Bacteria play an important role in the onset and perpetuation of intestinal inflammation inflammatory bowel disease (IBD). Unlike Crohn9s (CD), which dysbiosis has been better characterised, ulcerative colitis (UC), only small cohorts have studied showed conflicting data. Therefore, we evaluated a large cohort if microbial signature described CD is also present UC, could characterise predominant UC. To assess functional impact dysbiosis, quantified bacterial metabolites....
Many species within the phylum Firmicutes are thought to exert anti-inflammatory effects. We quantified bacteria belonging genus Butyricicoccus in stools of patients with ulcerative colitis (UC) and Crohn's disease (CD). evaluated effect pullicaecorum a rat model analysed ability prevent cytokine-induced increases epithelial permeability.A genus-specific quantitative PCR was used for quantification from UC or CD healthy subjects. The B on trinitrobenzenesulfonic (TNBS)-induced assessed...
Summary To avoid detrimental interactions with intestinal microbes, the human epithelium is covered a protective mucus layer that traps host defence molecules. Microbial properties such as adhesion to further result in unique mucosal microbiota great potential interact host. As microbes are difficult study vivo , we incorporated mucin‐covered microcosms dynamic vitro gut model, simulator of microbial ecosystem (SHIME). We assessed importance environment this M‐SHIME (mucosal‐SHIME) for...
ABSTRACT In broiler chickens, feed additives, including prebiotics, are widely used to improve gut health and stimulate performance. Xylo-oligosaccharides (XOS) hydrolytic degradation products of arabinoxylans that can be fermented by the microbiota. current study, we aimed analyze prebiotic properties XOS when added diet. Administration in addition a wheat-rye-based diet, significantly improved conversion ratio. increased villus length ileum. It also numbers lactobacilli colon Clostridium...
Objective Data from clinical research suggest that certain probiotic bacterial strains have the potential to modulate colonic inflammation. Nonetheless, these data differ between studies due used and poor knowledge of their mechanisms action. Design By mass-spectrometry, we identified quantified free long chain fatty acids (LCFAs) in probiotics assessed effect one them mouse colitis. Results Among all LCFAs by mass spectrometry Escherichia coli Nissle 1917 (EcN), a for treatment multiple...
Intestinal health is critically important for the digestion and absorption of nutrients thus a key factor in determining performance. issues are very common high performing poultry lines due to feed intake, which puts pressure on physiology digestive system. Excess not digested absorbed small intestine may trigger dysbiosis, i.e. shift microbiota composition intestinal tract. Dysbiosis as well other stressors elicit an inflammatory response loss integrity tight junctions between epithelial...
In broiler chickens, a diet where the major cereal types are wheat, rye and/or barley has lower digestibility compared with in which maize is type. present study, effects of two different dietary types, v. wheat/rye, on host factors (inflammation and gut integrity) microbiota composition were studied. addition, low-dose Zn-bacitracin supplementation examined. Broilers given wheat/rye-based showed more villus fusion, thinner tunica muscularis, T-lymphocyte infiltration, higher amount immune...
Pouchitis is the most common complication after colectomy with ileal pouch-anal anastomosis (IPAA) for UC and risk highest within 1st year surgery. The pathogenesis not completely understood but clinical response to antibiotics suggests a role gut microbiota. We hypothesised that pouchitis can be predicted based on faecal microbial composition before colectomy.Faecal samples from 21 patients undergoing IPAA were prospectively collected at predefined visits 1 month, 3 months, 6 months 12...
Summary Sixteen butyrate‐producing bacteria were isolated from the caecal content of chickens and analysed phylogenetically. They did not represent a coherent phylogenetic group, but allied to four different lineages in Firmicutes phylum. Fourteen strains appeared novel species, based on level ≤ 98.5% 16S rRNA gene sequence similarity towards their nearest validly named neighbours. The highest butyrate concentrations produced by belonging clostridial clusters IV XIVa, which are predominant...
Butyricicoccus is a butyrate-producing clostridial cluster IV genus whose numbers are reduced in the stool of ulcerative colitis [UC] patients. Conditioned medium [B.] pullicaecorum prevents tumour necrosis factor alpha [TNFα]-induced increase epithelial permeability vitro. Since butyrate influences intestinal barrier integrity, we further investigated relationship between abundance mucosa-associated and expression butyrate-regulated tight junction [TJ] genes.Tight protein 1 [TJP1], occludin...
Probiotics which do not result in the development and spread of microbial resistance are among candidate replacements for antibiotics previously used as growth promotors. In this study effect in-feed supplementation butyrate producing Butyricicoccus pullicaecorum strain 25-3T on performance, intestinal microbiota prevention necrotic enteritis (NE), a disease caused by Clostridium perfringens was evaluated broilers. For performance study, day old Ross 308 chicks were randomly allocated into...
Advances in gut microbiota research have triggered interest developing colon butyrate producers as niche-specific next-generation probiotics, targeted at increasing production and countering disease-associated alterations. Crucial steps the development of probiotics are design formulations with a reasonable shelf life well safety demonstration an intervention healthy volunteers. One such potential butyrate-producing probiotic is Butyricicoccus pullicaecorum 25-3T, demonstrated vitro animal...
Both mycotoxin contamination of feed and Clostridium perfringens-induced necrotic enteritis have an increasing global economic impact on poultry production. Especially the Fusarium deoxynivalenol (DON) is a common contaminant. This study aimed at examining predisposing effect DON development in broiler chickens. An experimental perfringens infection revealed that DON, level 3,000 to 4,000 µg/kg feed, increased percentage birds with subclinical from 20±2.6% 47±3.0% (P<0.001). significantly...
Strains LMG 27428 T and 27427 were isolated from the caecal content of a chicken produced butyric, lactic formic acids as major metabolic end products. The genomic DNA G+C contents strains 40.4 38.8 mol%. On basis 16S rRNA gene sequence similarity, both most closely related to generically misclassified Streptococcus pleomorphus ATCC 29734 . Strain could be distinguished S. based on production more acid less in M2GSC medium, higher absence activities phosphatase leucine, arginine, leucyl...
Five isolates that produced large amounts of butyrate were obtained in the course a study on butyrate-producing microbiota from caecal content 4-week-old broiler chicken. The five virtually indistinguishable biochemical and genetic terms, suggesting they derived single bacterial clone colonizing this habitat. A phylogenetic analysis based 16S rRNA gene sequences demonstrated represented unique lineage within Clostridium leptum subgroup clostridia, with Eubacterium desmolans as closest...
Salmonella enterica serotype Enteritidis is the predominant associated with egg-borne salmonellosis in humans. Apparently this possesses particular characteristics that increase its chance to contaminate eggs. To identify these characteristics, two strains as well one strain of each serotypes Typhimurium, Heidelberg, Virchow and Hadar were used examine different aspects related egg contamination. After an intravenous infection laying hens, it was observed ability colonize reproductive organs...
The objective of this in vivo experiment was to evaluate the influence arabinoxylooligosaccharides (AXOS) on shedding and colonization Salmonella Enteritidis broilers. Arabinoxylooligosaccharides, which are oligosaccharides derived from arabinoxylans by partial hydrolysis, have a beneficial effect feed conversion ratios when added broiler diets. Additionally, AXOS been shown promote growth bifidobacteria cecocolonic compartment gastrointestinal tract. To investigate impact broilers with...