A5059615023- Skin and Cellular Biology Research
- Cytomegalovirus and herpesvirus research
- NF-κB Signaling Pathways
- RNA regulation and disease
- Cell Adhesion Molecules Research
- Toxin Mechanisms and Immunotoxins
- RNA Interference and Gene Delivery
- Cellular Mechanics and Interactions
- Autoimmune Bullous Skin Diseases
- Cutaneous lymphoproliferative disorders research
- Dermatology and Skin Diseases
- MicroRNA in disease regulation
- Wnt/β-catenin signaling in development and cancer
- Peroxisome Proliferator-Activated Receptors
- Coagulation, Bradykinin, Polyphosphates, and Angioedema
- Viral Infections and Immunology Research
- Rheumatoid Arthritis Research and Therapies
- Nail Diseases and Treatments
- Metabolism and Genetic Disorders
- Cancer-related molecular mechanisms research
- Plant Reproductive Biology
- Pluripotent Stem Cells Research
- Polysaccharides and Plant Cell Walls
- Retinoids in leukemia and cellular processes
- Extracellular vesicles in disease
Inserm
2006-2024
Université de Toulouse
2005-2024
Université Toulouse III - Paul Sabatier
2005-2022
Centre Hospitalier Universitaire de Toulouse
2007-2021
Centre National de la Recherche Scientifique
2004-2016
Centre de Physiopathologie de Toulouse-Purpan
2015-2016
Hôpital Purpan
2010
Centre for Human Genetics
2000-2002
University of Oxford
2000-2001
Université Côte d'Azur
1998
Objective Data from clinical research suggest that certain probiotic bacterial strains have the potential to modulate colonic inflammation. Nonetheless, these data differ between studies due used and poor knowledge of their mechanisms action. Design By mass-spectrometry, we identified quantified free long chain fatty acids (LCFAs) in probiotics assessed effect one them mouse colitis. Results Among all LCFAs by mass spectrometry Escherichia coli Nissle 1917 (EcN), a for treatment multiple...
Epidermolysis bullosa simplex with muscular dystrophy (MD-EBS) is a disease characterized by generalized blistering of the skin associated involvement. We report that three MD-EBS patients not reactive antibodies 6C6, 10F6, or 5B3 raised against intermediate filament-associated protein plectin. Immunofluorescence and Western analysis explanted keratinocytes confirmed deficient expression plectin, which, in involved skin, correlated an impaired interaction keratin cytoskeleton hemidesmosomes....
Plectin is a widely expressed cytomatrix component involved in the attachment of cytoskeleton to plasma membrane. We have recently reported that skin and muscles three patients affected by epidermolysis bullosa simplex with muscular dystrophy (MD-EBS), genetic disorder characterized blistering associated muscle involvement, are not reactive antibodies specific plectin. demonstrated skin, lack plectin leads failure keratin filaments connect membrane via hemidesmosomes, whereas deficient...
Congenital human cytomegalovirus (HCMV) infection is a leading cause of birth defects, primarily manifesting as neurological disorders. HCMV alters expression cellular microRNAs (miRs) and induces cell cycle arrest, which in turn modifies the environment to favor virus replication. Previous observations found that reduces miR-21 neural progenitor/stem cells (NPCs). Here, we show NPCs U-251MG represses while increasing levels Cdc25a, regulator known target miR-21. These opposing responses...
Background Human cytomegalovirus (HCMV) encodes microRNAs (miRNAs) that function as post-transcriptional regulators of gene expression during lytic infection in permissive cells. Some miRNAs have been shown to suppress virus replication, which could help HCMV establish or maintain latent infection. However, miRNA has not comprehensively examined and compared using cell culture systems representing (lytic) semi-permissive vs. non-permissive (latent-like) Methods Viral levels kinetics were...
Deimination (or citrullination) is a recently described post-translational modification, but its consequences are not yet well understood. It catalysed by peptidylarginine deiminases (PADs). These enzymes transform arginyl residues involved in peptidyl link into citrullyl calcium-dependent manner. Several PAD substrates have already been identified like filaggrin and keratins K1 K10 the epidermis, trichohyalin hair follicles, also ubiquitous proteins histones. PADs act large panel of...
ABSTRACT Human cytomegalovirus (HCMV) infection of the developing fetus frequently results in major neural developmental damage. In previous studies, HCMV was shown to downregulate progenitor/stem cell (NPC) markers and induce abnormal differentiation. As Notch signaling plays a vital role maintenance stem status is switch that governs NPC differentiation, effect on pathway NPCs investigated. downregulated mRNA levels Notch1 its ligand, Jag1, reduced protein altered intracellular...
Congenital infection by human cytomegalovirus (HCMV) is a leading cause of permanent sequelae the central nervous system, including sensorineural deafness, cerebral palsies or devastating neurodevelopmental abnormalities (0.1% all births). To gain insight on impact HCMV neuronal development, we used both neural stem cells from embryonic (NSC) and brain sections infected fetuses investigated outcomes Peroxisome Proliferator-Activated Receptor gamma (PPARγ), transcription factor critical in...
Optic neuropathies are characterized by the degeneration of optic nerves and represent a considerable individual societal burden. Notably, Leber's hereditary neuropathy (LHON) is devastating vision disease caused mitochondrial gene mutations that hinder oxidative phosphorylation increase stress, leading to loss retinal ganglion neurons axons. Loss rapid severe, predominantly in young adults. Penetrance incomplete, time onset unpredictable. Recent findings revealed incidence genetic LHON...
Transcription control at a distance is critical mechanism, particularly for contiguous genes. The peptidylarginine deiminases (PADs) catalyse the conversion of protein-bound arginine into citrulline (deimination), reaction in pathophysiology multiple sclerosis, Alzheimer's disease and rheumatoid arthritis, metabolism major epidermal barrier protein filaggrin, strong predisposing factor atopic dermatitis. PADs are encoded by 5 clustered PADI genes (1p35-6). Unclear mechanisms controlling...
A general improvement with ageing has been reported in a few cases of epidermolysis bullosa pyloric atresia (PA-JEB), an autosomal recessive skin disease characterized by extensive disadhesion epithelia. In patient who improved from severe to mild PA-JEB, search for mutations the integrin beta4 gene (IGTB4) detected heterozygosity novel base substitution 3986-19T-->A putative branchpoint sequence intron 31, and point mutation 3802+1G-->A donor splice site 30 previously associated PA-JEB....
Human peptidylarginine deiminase type III gene (PADI3) encodes a crucial post-translational modification enzyme that converts protein-bound arginine residues into citrulline residues. Its expression is restricted to few cell types, including keratinocytes in the granular layer of epidermis and inner root sheath hair follicles. In these cells, involved terminal processing intermediate filament-binding proteins such as filaggrin trichohyalin. To study molecular mechanisms control PADI3 human...