A5003033360- Virus-based gene therapy research
- Neuroinflammation and Neurodegeneration Mechanisms
- Neurogenesis and neuroplasticity mechanisms
- RNA Interference and Gene Delivery
- Neurological Disease Mechanisms and Treatments
- Lysosomal Storage Disorders Research
- Neurological disorders and treatments
- Nerve injury and regeneration
- Neonatal and fetal brain pathology
- Nuclear Receptors and Signaling
- Autism Spectrum Disorder Research
- Meningioma and schwannoma management
- Acute Ischemic Stroke Management
- Viral Infectious Diseases and Gene Expression in Insects
- CRISPR and Genetic Engineering
- Glioma Diagnosis and Treatment
- Axon Guidance and Neuronal Signaling
- Airway Management and Intubation Techniques
- Intraoperative Neuromonitoring and Anesthetic Effects
- Barrier Structure and Function Studies
- Anesthesia and Neurotoxicity Research
- Thyroid and Parathyroid Surgery
- Inflammatory Myopathies and Dermatomyositis
- Medicinal Plant Pharmacodynamics Research
- Brain Metastases and Treatment
Jichi Medical University
2016-2024
Ashikaga Red Cross Hospital
2021
Koga Hospital
2020
Albert Einstein College of Medicine
2013
Sakurabashi Watanabe Hospital
2009
Okayama University
2005-2008
Okayama Shoka University
2006
Restoring dopamine production in the putamen through gene therapy is a straightforward strategy for ameliorating motor symptoms Parkinson's disease (PD). In 1-methyl-4-phenyl-1,2,3,6-tetrahydropyridine (MPTP) toxicity-based primate model of PD, we previously showed safety and efficacy adeno-associated viral (AAV) vector-mediated delivery to three dopamine-synthesizing enzymes (tyrosine hydroxylase [TH], aromatic l-amino acid decarboxylase [AADC], guanosine triphosphate cyclohydrolase I...
Transient global ischemia causes selective, delayed death of hippocampal CA1 pyramidal neurons in humans and animals. It is well established that estrogens ameliorate neuronal animal models focal ischemia. However, the role signal transducer activator transcription-3 (STAT3) its target genes estradiol neuroprotection remains unclear. Here we show a single intracerebral injection 17β-estradiol to ovariectomized female rats immediately after rescues destined die. Ischemia promotes activation...
For brain tissue regeneration, any scaffold for migrated or transplanted stem cells with supportive angiogenesis is important once necrotic has formed a cavity after injury such as cerebral ischemia. In this study, new porous gelatin–siloxane hybrid derived from the integration of gelatin and 3-(glycidoxypropyl) trimethoxysilane was implanted three-dimensional into defect cortex. The lesion remained at same site 60 days, kept integrity shape, attached well to surrounding tissues. Marginal...
Fabry disease (FD) is an inherited lysosomal storage caused by deficiency of α-galactosidase A (α-Gal A), enzyme that hydrolyzes glycosphingolipids in lysosome. Accumulation glycosphingolipids, mainly globotriaosylceramide (Gb3) tissues, induces cellular dysfunction leading to multi-organ disorder. Gene therapy a promising strategy can overcome these problems, and virus vectors such as adeno-associated (AAV) have been used for study on gene therapy. We human Gb3 synthetase-transgenic...
Abstract Recent studies have shown that administration of granulocyte colony‐stimulating factor (G‐CSF) is neuroprotective. However, the precise mechanisms neuroprotective effect G‐CSF are not entirely known. We carried out 90‐min transient middle cerebral occlusion (tMCAO) rats. The rats were injected with vehicle or (50 μg/kg) immediately after reperfusion and sacrificed 8, 24, 72 hr later. 2,3,5‐Triphenyltetrazolium chloride (TTC) staining was using brain sections hr, immunohistochemistry...
Idiopathic pulmonary fibrosis (IPF) is a fibroproliferative disorder with limited therapeutic options. An aberrant wound healing process in response to repetitive lung injury has been suggested for its pathogenesis, and number of cytokines including transforming growth factor β1 play pivotal roles the induction progression fibrosis. Thus, regulation these pro-inflammatory conditions may reduce IPF ameliorate symptoms patients. Interleukin-10 (IL-10), pleiotropic cytokine, exerts...
ABSTRACT Purpose We examined the feasibility of using a head‐mounted display (HMD) to improve ergonomics sonographic‐guided interventional procedures. Methods Five physicians with experience more than 20 central venous catheterizations participated in this study. Each participant performed five pairs simulated jugular vein catheterization under sonographic guidance and without HMD. The procedure time was determined as well number head movements, needle redirections, posterior wall punctures,...
Adeno-associated virus (AAV) vectors are promising tools for gene therapy. The current AAV vector system produces an abundance of empty capsids that eliminated before clinical use, leading to increased costs In the present study, we established production regulates timing capsid expression using a tetracycline-dependent promoter. Tetracycline-regulating viral yield and reduced in various serotypes without altering infectivity vitro vivo. replicase pattern change observed developed improved...
Adoptive transfer of T cells expressing a chimeric antigen receptor (CAR) is promising cell-based anticancer therapy. Although clinical studies this approach show therapeutic efficacy, additional genetic modification necessary to enhance the efficacy and safety CAR-T cells. For example, production an antitumor cytokine from can potentially their tumor-killing activity, but there are concerns that constitutive expression molecules will cause systemic side effects. Therefore, it important...
The purpose of this study was to examine the feasibility an optical see-through head-mounted display (OST-HMD) improve ergonomics during ultrasound-guided fine-needle aspiration (FNA) in neck region.This randomized controlled compared OST-HMD with a normal ultrasound monitor FNA region. Patients tumor were recruited and into one two groups. Two practitioners performed or without HMD, as indicated. An independent researcher measured procedure time, number time head movements, well needle...
Abstract Survivin, a member of the inhibitors apoptosis protein gene family, inhibits activity caspase, leading to halt apoptotic process. Our study focused on neuroprotective effect survivin after transient middle cerebral artery occlusion ( MCAO ) with intraparenchymal administration an adeno‐associated virus AAV vector. His‐tagged was cloned and packaged into ‐rh10 Four‐week‐old Sprague–Dawley rats were injected 4 × 10 9 vg AAV‐GFP or ‐His‐survivin right striatum treated 3 weeks later for...
Autonomic dysfunction in patients with Parkinson's disease has been recognized since the original description by James Parkinson 1817. In present case, an 80-year-old woman who had diagnosed 3 years earlier (Hoehn and Yahr stage III) was admitted a few days history of lethargy bradykinesia. She lived heated house used electric blanket at night. On examination, her core temperature 29.7°C. Her initial ECG showed sinus bradycardia, QT prolongation, Osborn waves, which disappeared after...
Abstract Background Fabry disease (FD) is an inherited lysosomal storage caused by deficiency of α‐galactosidase A (α‐Gal A) encoded the GLA gene. The symptoms FD occur as a result accumulation globotriaosylceramide (Gb3), comprising substrate α‐Gal A, in organs. Adeno‐associated virus (AAV)‐mediated gene therapy promising treatment for FD. Methods knockout (GLAko) mice were injected intravenously with AAV2 (1 × 10 11 viral genomes [vg]) or AAV9 2 12 vg) vectors carrying human (AAV‐hGLA),...
An 80-year-old man was admitted for acute subdural hematoma caused by a mild brain injury. His coagulation test showed an isolated prolongation of activated partial thromboplastin time (aPTT). Though the did not progress, oozing bleed from wound tracheostomy continued. Failure correction on aPTT mixing supported presence inhibitor to factor. Once diagnosis acquired hemophilia A (AHA) made, steroid therapy performed, which leads him complete remission AHA. Isolated can be key diagnose rare...
Reduction of brain plasticity underlies the poor outcome aged stroke patients. The molecular mechanism reduction by aging is uncertain, but disturbed lipid metabolism may be implicated.We investigated expression low density lipoprotein receptors (LDL-R) and apolipoprotein E (ApoE), both which play active roles in young adult old rat brains after ischemia.LDL-R, trivially expressed sham-operated neurons, was increased from day 1 became prominent at days 7 21 peri-ischemic cortex. magnitude...