A5064351460- RNA Research and Splicing
- RNA and protein synthesis mechanisms
- Cancer, Hypoxia, and Metabolism
- Animal Virus Infections Studies
- Angiogenesis and VEGF in Cancer
- Immunotherapy and Immune Responses
- RNA modifications and cancer
- Viral gastroenteritis research and epidemiology
- Cancer, Lipids, and Metabolism
- Cancer Immunotherapy and Biomarkers
- interferon and immune responses
- Acute Myeloid Leukemia Research
- Genomics and Rare Diseases
- Plant Virus Research Studies
- Genomics and Chromatin Dynamics
- Virus-based gene therapy research
- Genetics and Neurodevelopmental Disorders
- Cytokine Signaling Pathways and Interactions
- Single-cell and spatial transcriptomics
- SARS-CoV-2 and COVID-19 Research
- Phagocytosis and Immune Regulation
- Neuroblastoma Research and Treatments
- Molecular Biology Techniques and Applications
- T-cell and Retrovirus Studies
- Plant Micronutrient Interactions and Effects
Northwestern University
2023-2025
Yantai Academy of Agricultural Sciences
2025
National Human Genome Research Institute
2025
Massachusetts Institute of Technology
2023-2024
Nanjing Medical University
2023-2024
Ludong University
2022-2023
Tsinghua University
2022
Georgetown University Medical Center
2020-2022
Georgetown University
2020-2022
Sichuan Agricultural University
2019-2020
Abstract Transcription is the primary regulatory step in gene expression. Divergent transcription initiation from promoters and enhancers produces stable RNAs genes unstable 1,2 . Nascent RNA capture sequencing assays simultaneously measure enhancer activity cell populations 3 However, fundamental questions about temporal regulation of enhancer–gene coordination remain unanswered, primarily because absence a single-cell perspective on active transcription. In this study, we present...
Riboswitches are ligand-responsive gene-regulatory RNA elements that perform key roles in maintaining cellular homeostasis. Understanding how riboswitch sensitivity to ligand (EC50) is controlled critical explain highly conserved aptamer domains deployed a variety of contexts with different demands. Here we uncover by which folding dynamics control cells. By investigating the Clostridium beijerinckii pfl ZTP riboswitch, identify multiple mechanistic routes altering expression platform...
Nucleic acid-sensing Toll-like receptors (TLR) 3, 7/8, and 9 are key innate immune sensors whose activities must be tightly regulated to prevent systemic autoimmune or autoinflammatory disease virus-associated immunopathology. Here, we report a systematic scanning-alanine mutagenesis screen of all cytosolic luminal residues the TLR chaperone protein UNC93B1, which identified both negative positive regulatory regions affecting TLR3, TLR7, TLR9 responses. We subsequently two families harboring...
Porcine deltacoronavirus (PDCoV) is a newly emerging enteric pathogen in swine that causes diarrhea neonatal piglets and creates an additional economic burden on porcine industries Asia North America. In this study, PDCoV isolate, CHN-SC2015, was isolated from Sichuan Province southwest China. The isolate characterized by cytopathic effect, immunofluorescence, electron microscopy. CHN-SC2015 titers LLC-PK cells ranged 104.31 to 108.22 TCID50/mL during the first 30 passages. During serial...
The mechanistic pathways that give rise to the extreme symptoms exhibited by rare disease patients are complex, heterogeneous, and difficult discern. Understanding these mechanisms is critical for developing treatments address underlying causes of diseases rather than merely presenting symptoms. Moreover, same dysfunctional series interrelated implicated in recessive may also lead milder potentially preventable carriers general population. Seizures a common phenotype can result from diverse...
Granzyme B is an immune-related biomarker that closely correlates with cytotoxic T lymphocytes (CTLs), and hence detecting the expression level of granzyme can provide a dependable scheme for clinical immune response assessment. In this study, two positron emission tomography (PET) probes [18F]SF-M-14 [18F]SF-H-14 targeting are designed based on intramolecular cyclization scaffold SF. respond to glutathione (GSH) conduct self-assemble into nanoaggregates enhance retention probe at target...
Porcine deltacoronavirus (PDCoV), first identified in 2012, is a swine enteropathogen now found many countries. The nucleocapsid (N) protein, core component of PDCoV, essential for virus replication and significant candidate the development diagnostics PDCoV. In this study, monoclonal antibodies (mAbs) were generated tested reactivity with three truncations full protein (N1, N2, N3) that contained partial overlaps; five monoclonals chosen tested, each reacted only N3 truncation. antibody...
Covid-19 (SARS CoV-2) has become a deadly, world-wide pandemic. Although most who are infected survive, complications from the virus can be pronounced and long-lasting. To date, of all respiratory viruses including influenza coronaviruses, only had drug (i.e., Tamiflu) specifically targeted to treat prevent infection. As result, additional agents that target viral production clinically feasible needed alleviate infections. The idea using miRNA/siRNA molecular approach for treating various...
Riboswitches are ligand-responsive gene-regulatory RNA elements that perform key roles in maintaining cellular homeostasis. Understanding how riboswitch sensitivity is controlled critical to understanding highly conserved aptamer domains deployed a variety of contexts with different demands. Here we uncover new by which folding dynamics control cells. By investigating the
Causes and treatments for heart failure (HF) have been investigated over a century culminating in data that led to numerous pharmacological surgical therapies. Unfortunately, date, even with the most current treatments, HF remains progressive disease no therapies targeting cardiomyocytes directly. Technological advances within past two three years brought about new paradigms treating many diseases previously had extremely difficult resolve. One of these has shift from agents antisense...
Abstract Transcription is the primary regulatory step in gene expression. Divergent transcription initiation from promoters and enhancers produces stable RNAs genes unstable 1–5 . Nascent RNA capture sequencing assays simultaneously measure enhancer activity cell populations 6–9 However, fundamental questions temporal regulation of enhancer-gene synchrony remain unanswered primarily due to absence a single-cell perspective on active transcription. In this study, we present scGRO-seq - novel...
Over the long journey of understanding micro-world, cryo- EM has become an effective technique for biomolec-ular structure determinations. However, due to complex algorithmic features and large amounts computing data, so-phisticated HPC solutions are in urgent demand. In this paper, we present our efforts porting RELION new generation Sunway supercomputer. Optimizations that fit well with hardware architecture have been proposed, including a multi-level parallel scheme smartly maps scales...
Pancreatic ductal adenocarcinoma (PDAC) is an aggressive cancer without effective treatments. It characterized by activating KRAS mutations and p53 alterations. However, how these dysregulate cancer-cell-intrinsic gene programs to influence the immune landscape of tumor microenvironment (TME) remains poorly understood. Here, we show that
Cell surface molecules PD-L1 and CD47 are potent inhibitors of adaptive innate anti-cancer immunity. We sought to generate a safe, therapeutic, bispecific antibody specifically targeting, blocking both inhibitory activity. Novel anti-PDL-1 anti-CD47 antibodies with favorable activity, were humanized constructed into unique bi-specific intended for clinical use. Previous pre-clinical studies using indicated anemia thrombocytopenia as potential risks. QL401 is x engineered reduce effect on red...