Mycotoxin-induced liver injury is often accompanied by oxidative stress (OS) and inflammation. This research aimed to explore the potential mechanism of sodium butyrate (NaBu) in modulating hepatic anti-oxidation anti-inflammation pathways deoxynivalenol (DON)-exposed piglets. The results show that DON induced injury, increased mononuclear cell infiltration, decreased serum total protein albumin concentrations. Transcriptomic analysis revealed reactive oxygen species (ROS) TNF-α were highly...

Porcine deltacoronavirus (PDCoV) is a newly emerging enteric pathogen in swine that causes diarrhea neonatal piglets and creates an additional economic burden on porcine industries Asia North America. In this study, PDCoV isolate, CHN-SC2015, was isolated from Sichuan Province southwest China. The isolate characterized by cytopathic effect, immunofluorescence, electron microscopy. CHN-SC2015 titers LLC-PK cells ranged 104.31 to 108.22 TCID50/mL during the first 30 passages. During serial...

Heat stress (HS) results from excessive heat load on animals such that all adaptive mechanisms used to dissipate the do not return body normal temperature. In pigs, HS in increased fat deposition compared with pair-fed a thermoneutral environment. Although there is evidence increases activity of lipoprotein lipase (LPL) adipose tissue stressed fundamental causes adiposity are still unknown. It remains unclear whether directly alters metabolism adipocytes. Therefore, understand mechanism...

Heat stress (HS) negatively affects all aspects of performance in pigs. Although certain tissue-specific responses the liver, skeletal muscle, and intestine are known, there is paucity information on within adipose tissue. Therefore, objective this study was to delineate tissue during HS Thirty crossbred (Ossabaw × Duroc Landrace) pigs were assigned 3 treatments for 7 d. Treatments 1) control libitum fed (CON) with room temperature set at 20°C ± 1°C, 2) pair (PF) as CON treatment but pigs,...

To determine the effect of heat stress (HS) on adipose tissue metabolome, a combination liquid chromatography-mass spectrometry-based metabolomics profiling approaches was applied to characterize changes metabolite classes in adipocytes differentiated culture (in vitro) and mesenteric pigs exposed HS vivo). Effect composition individual fatty acids cultured adipocytes, tissue, serum animals also investigated using gas chromatography analysis. In vitro, preadipocytes were either under control...

Elevated ambient temperature causes heat stress in pigs, resulting reduced animal performance. To better understand tissue responses to we conducted a study which pigs were subjected four treatments: acute (24 h) (AHS) at 35 °C ± 1 temperature, chronic (7 d) (HS) or normal (20 °C± 1) for 7 d with ad-libitum feeding (Con) pair-feeding the feed intake (FI) of HS (PF). Heat decreased FI by approximately 36% and 64% AHS treatments respectively, compared Con (P < 0.01). Concentration free fatty...

Abstract Background Cholesterol is an essential component of lipid rafts in cell plasma membrane, which exerts a hepatoprotective role against mycotoxin exposure pigs, and cholesterol metabolism vulnerable to epigenetic histone acetylation. Therefore, our present study aimed investigate whether deacetylase inhibitor (sodium butyrate [NaBu]) could protect the porcine liver from deoxynivalenol (DON) by modulating metabolism. Herein, we randomly divided 28 pigs into four groups, were fed...

Porcine deltacoronavirus (PDCoV), first identified in 2012, is a swine enteropathogen now found many countries. The nucleocapsid (N) protein, core component of PDCoV, essential for virus replication and significant candidate the development diagnostics PDCoV. In this study, monoclonal antibodies (mAbs) were generated tested reactivity with three truncations full protein (N1, N2, N3) that contained partial overlaps; five monoclonals chosen tested, each reacted only N3 truncation. antibody...

The serum level of cholesterol and its biosynthetic intermediates are critical indicators to access metabolism-related disorders in humans animals. However, the molecular actions these on gene functions regulation remained elusive. Here, we show that desmosterol (DES) is most abundant intermediate involved biosynthesis highly enriched red/brown algae. It exerts a pivotal role modulating core genes oxidative stress inflammatory response processes ileum epithelial cells (IPI-2I). We observed...

Several genome‐wide association studies ( GWASs ) in Caucasian populations have identified 12 loci that are significantly associated with migraine. More evidence suggests serotonin receptors also involved migraine pathophysiology. In the present study, a case–control study was conducted cohort of 581 cases and 533 ethnically matched controls among Chinese population. Eighteen polymorphisms from were selected, genotyping performed using Sequenom MALDI‐TOF mass spectrometry iPLEX platform. The...

Hepatocyte growth factor (HGF) was identified as an endogenous tissue protective agent against apoptosis in many cell types. The mechanism by which HGF protects primary endothelial cells (ECs) has not yet been completely elucidated. FOXO1 and FOXO3a, two members of the FOXO family, are most abundant isoforms mature cells. In this study, we aimed to explore whether FOXO3a play similar roles HGF-mediated protection Our result showed that prevented ECs from oxidative-stress induced part...

Porcine epidemic diarrhea virus (PEDV) is an alphacoronavirus that causes acute inflammation and severe in newborn piglets with a high mortality rate. Given cholesterol required for coronavirus infection vitro, the role of endogenous metabolism regulating mechanism behind it ought to be elucidated. In this study, we found levels bile acids were both elevated livers PEDV-infected compared those control group. Consistently, piglets, expression key genes involved was significantly increased....

Porcine epidemic diarrhea virus (PEDV) is currently detected as the main pathogen causing severe in pig farms. The phenotypic alterations induced by pathogenic infections are usually tightly linked with marked changes epigenetic modification and gene expression. We performed global mapping of H3K4 trimethylation (H3K4me3) transcriptomic analyses jejunum PEDV-infected healthy piglets using chromatin immunoprecipitation sequencing RNA-seq techniques. A total 1885 H3K4me3 peaks that associated...

Zearalenone (ZEA) is one of the main mycotoxins widely spread in contaminated cereal crops, which poses a great threat to food safety as well human and animal health. Biological control strategies are emerging important solutions eliminate mycotoxin contaminations. However, molecular mechanisms underlying ZEA cytotoxic effects only partly understood. Noncoding RNAs chromatin accessibilities regulators gene expression implicate variety biological processes. Here, we established study model...

As a member of alpha-coronaviruses, PEDV could lead to severe diarrhea and dehydration in newborn piglets. Given that lipid peroxides the liver are key mediators cell proliferation death, role regulation endogenous peroxide metabolism response coronavirus infection need be illuminated. The enzymatic activities SOD, CAT, mitochondrial complex-I, complex-III, complex-V, along with glutathione ATP contents, were significantly decreased In contrast, peroxidation biomarkers, malondialdehyde, ROS...

Ochratoxin A (OTA) is one of the mycotoxins that poses a serious threat to human and animal health. Curcumin (CUR) major bioactive component turmeric provides multiple health benefits. CUR can reduce toxicities induced by mycotoxins, but underlying molecular mechanisms remain largely unknown. To explore effects on OTA toxicity identify key regulators metabolites involved in biological processes, we performed metabolomic transcriptomic analyses livers from OTA-exposed mice. We found alleviate...

This study evaluated the effect of fermented soybean meal (FSBM) supplementation on growth performance, meat quality, blood biochemical parameters, and fecal microflora finishing pigs. Thirty-two crossbred pigs (Duroc × Landrance Yorkshire) (66-day-old, 67.95±0.25 kg) were randomly allocated to two treatments fed diets containing FSBM. The average daily gain (ADG), feed intake (ADFI), conversion ratio (FCR), quality index measured. At end experiment, [...]

Disruption of lipid metabolism is largely linked to metabolic disorders, such as hypercholesterolemia (HCL) and liver steatosis. While cholesterol re-programmers can serve targets for relevant interventions. Here we explored the dietary conjugated linoleic acids (CLA)-induced HCL in mice molecular regulation behind it.A high dose CLA supplementation diet was used induce found cause a hyper-activated biosynthesis programme liver, leading dysregulation. The effects small-molecule drug...

Porcine epidemic diarrhea virus (PEDV) is an emerging coronavirus which causes acute and destroys gastrointestinal barrier function in neonatal pigs. Trefoil factor 1 (TFF1) a protective peptide for maintaining the integrity of mucosa reducing intestinal inflammation. However, its role protecting epithelium against PEDV infection still unclear. In this study, we discovered that TFF1 expression was activated jejunum pigs with required growth porcine epithelial cells. For instance, inhibited...

Heat stress (HS) leads to increased lipid storage and expression of cytosolic phosphoenolpyruvate carboxykinase (PCK1) in pig adipocytes. However, the importance PCK1 activation adaptive response HS is unknown. Therefore, vitro experiments were conducted investigate effect inhibition with 3-mercaptopicolinic acid (3MPA) on adipocyte during HS. In culture adipocytes under (41.0 °C) (P < 0.05) triacylglycerol accumulation compared control (37.0 °C). reactive oxygen species level 3MPA further...

This study investigated the use for bamboo vinegar powder as an antibiotic alternative in diet of growing-finishing pigs by examining their digestive bacterial communities. Forty-five Duroc × Landrace Yorkshire were randomly allocated to five groups: 0%, 0.5%, 1.0%, or 1.5% levels and antibiotics. After 37 days, digesta duodenum four from each treatment analyzed community compositions using 16S rRNA gene sequencing. The addition had effect on intestinal microflora most similar that...

Due to the limitations of eruption time and space, third molars (M3s) are often impacted induce a variety oral diseases, bringing adverse effects on health their adjacent second (M2s). For long time, large number studies have focused harm M3s (I-M3s) teeth, while less attention has been paid nonimpacted (N-M3s) that already erupted. In recent years, however, growing evidences shown existence N-M3s is also an important risk factor for various diseases whose hazard not taken seriously by...