A5051312766- Advanced Cellulose Research Studies
- Advanced Fluorescence Microscopy Techniques
- Surface Modification and Superhydrophobicity
- Advanced Biosensing Techniques and Applications
- Biosensors and Analytical Detection
- Material Properties and Processing
- Lipid Membrane Structure and Behavior
- Cellular transport and secretion
- Photosynthetic Processes and Mechanisms
- Electrowetting and Microfluidic Technologies
- Plant Reproductive Biology
- Advanced biosensing and bioanalysis techniques
- Immune Cell Function and Interaction
- Monoclonal and Polyclonal Antibodies Research
- Microfluidic and Capillary Electrophoresis Applications
- Cellular Mechanics and Interactions
- T-cell and B-cell Immunology
- Nanofabrication and Lithography Techniques
- Near-Field Optical Microscopy
- Chromosomal and Genetic Variations
- Microfluidic and Bio-sensing Technologies
- Ion channel regulation and function
- Glycosylation and Glycoproteins Research
- Cell Image Analysis Techniques
- Nanopore and Nanochannel Transport Studies
Technical University of Darmstadt
2015-2024
Merck (Germany)
2018-2021
Molina Center for Energy and the Environment
2012
Leiden University
2006-2011
National Institutes of Health
2009-2010
National Institute of Allergy and Infectious Diseases
2009
Amsterdam University of the Arts
2007
University of Amsterdam
2007
Wide-field single-molecule fluorescence microscopy has become an established tool for the study of dynamic biological processes which occur in plane a cellular membrane. In current we have extended this technique to three-dimensional analysis molecular mobility. Introduction cylindrical lens into emission path microscope produced some astigmatism was used obtain full position information. The localization accuracy fluorescent objects calculated theoretically and subsequently confirmed by...
Antibody affinity maturation, a hallmark of adaptive immune responses, results from the selection B cells expressing somatically hypermutated cell receptors (BCRs) with increased for antigens. Despite central role maturation in antibody molecular mechanisms by which antigen is translated into selective advantage that responses incompletely understood. We use high resolution live-cell imaging to provide evidence earliest BCR-intrinsic events follow within seconds BCR–antigen binding are...
Summary The relevance of endocytosis in plants against high turgor pressure has frequently been questioned on the basis energetic considerations. Here, we examine dynamics plasma membrane (PM) turgid guard cells Vicia faba by monitoring with confocal microscopy fate fluorescent styryl dyes (FM1‐43, FM2‐10 and FM4‐64). As a second marker, also observe retrieval chimaera K + ‐inward rectifying channel from Arabidopsis thaliana green protein (KAT1::GFP). Analysis cytoplasmic structures, which...
Several techniques are available to study chromosomes or chromosomal domains in nuclei of chemically fixed living cells.Current methods detect DNA sequences vivo limited trans interactions between a sequence and transcription factor from natural systems.Here, we expand live cell imaging tools using novel approach based on zinc finger-DNA recognition codes.We constructed several polydactyl finger (PZF) DNA-binding aimed recognize specific Arabidopsis mouse fused these with GFP.Plants cells...
Abstract The FcγRIIB is a potent inhibitory coreceptor that blocks BCR signaling in response to immune complexes and, as such, plays decisive role regulating Ab responses. recent application of high-resolution live cell imaging B studies providing new molecular details the earliest events initiation follow within seconds Ag binding. In this study, we report when colligated through complexes, colocalizes with microscopic clusters and initiate signaling, including oligomerization these...
Heterochromatin is the highly compacted form of chromatin with various condensation levels hallmarked by high DNA methylation. MeCP2 mostly known as a methylation reader but has also been reported heterochromatin organizer. Here, we combine liquid-liquid phase separation (LLPS) analysis and single-molecule tracking quantification local concentrations in vitro vivo to explore mechanism MeCP2-driven organization dynamics. We show that alone forms liquid-like spherical droplets via multivalent...
Summary Trafficking of K + inward (K in ) rectifying channels was analyzed guard cells Vicia faba transfected with the rectifier from Arabidopsis thaliana KAT1 fused to green fluorescent protein (GFP). Confocal images and whole‐cell patch‐clamp measurements confirmed incorporation active into plasma membrane cell protoplasts. The current density much larger protoplasts than wild‐type (wt) This shows a coupling between channel synthesis membrane. Pressure‐driven increase decrease surface area...
Summary It is a common belief that plant mesophyll cells are occupied up to 95% by single multipurpose vacuole. The ice plant, Mesembryanthemum crystallinum L., however, requires two contrasting functions of the vacuole under salt stress. Large amounts NaCl have be sequestered permanently for osmotic purpose and protecting cytoplasm from toxicity. A dynamic exchange with required because photosynthesis proceeds these conditions via metabolic cycle crassulacean acid metabolism (CAM)....
Imaging analyses and computer simulations suggest that a GPCR its G protein associate only in the presence of ligand.
Colour deconvolution is a method used in diagnostic brightfield microscopy to transform colour images of multiple stained biological samples into representing the stain concentrations. It applied by decomposing absorbance values mixtures single stains. The assumes linear relation between concentration and absorbance, which only valid under monochromatic conditions. Diagnostic applications, turn, are often performed polychromatic conditions, for an accurate result cannot be achieved. To show...
The properties of paper sheets can be tuned by adjusting the surface or bulk chemistry using functional polymers that are applied during (online) after (offline) papermaking processes. In particular, widely used to enhance mechanical strength wet state sheets. However, depends not only on chemical nature polymeric additives but also distribution polymer and in lignocellulosic paper. Here, we analyze photochemical attachment hydrophilic polydimethylacrylamide-co-methacrylate-benzophenone...
Cell targeting protein toxins have gained increasing interest for cancer therapy aimed at the therapeutic window and reducing systemic toxicity. Because recombinant expression of immunotoxins consisting a receptor-binding cell-killing moiety is hampered by their high toxicity in eukaryotic production host, most applications rely on fusion proteins an antibody fragment toxin bacterial hosts such as Escherichia coli (E. coli). These fusions often lack beneficial properties whole antibodies...
FcγRIIB functions to suppress the activation of immune cells. A single-nucleotide polymorphism in transmembrane (TM) domain FcγRIIB, FcγRIIB-T232, is associated with lupus. In this study, we investigated pathogenic mechanism FcγRIIB-T232 at both functional and structural levels. Our results showed that exhibited significantly reduced lateral mobility compared FcγRIIB-I232 was less enriched into microclusters complexes (ICs) after stimulation. However, if sufficient responding time given for...
Abstract To improve the reactivity of lignin for incorporation into high value polymers, introduction amines via Mannich reaction is a commonly used strategy. During this functionalization reaction, intra- as well intermolecular lignin–lignin crosslinking occurs, which can vastly change elastic properties lignin, and therefore, resulting polymer. Therefore, molecular structure amine that such may be great importance. However, relationship between lignin-based polymer generated thereof, has...
Abstract Since the pandemic outbreak of Covid-19 in December 2019, several lateral flow assay (LFA) devices were developed to enable constant monitoring regional and global infection processes. Additionally, innumerable test are frequently used for determination different clinical parameters, food safety, environmental factors. common LFAs rely on non-biodegradable nitrocellulose membranes, we focused their replacement by cellulose-composed, biodegradable papers. We report development...
Protocols were developed to automate image analysis and track the movement of thousands vesicular compartments in live cells. Algorithms used discriminate among different types (e.g. random, caged, directed). We applied these tools investigate steady-state distribution lytic granules (LG) natural killer (NK) cells by high-speed 3-dimensional (3D) spinning disc confocal 2-dimensional total internal reflection fluorescence microscopy. Both mouse NK a human cell line deficient small GTPase...
Impairment or stimulation of the immune system by ionizing radiation (IR) impacts on surveillance tumor cells and non-malignant can either foster therapy response side effects/toxicities therapy. For a better understanding mechanisms which IR modulates T-cell activation alters functional properties these cells, we exposed human immortalized Jurkat peripheral blood lymphocytes (PBL) to X-ray doses between 0.1 Gy 5 Gy. This resulted in cellular responses, are typically observed also naïve...
A simple, efficient, and repeatable combination of wax printing hot embossing is reported. This yields microfluidic channels in paper, where fluid transport driven by paper-intrinsic capillary forces takes place inside the noncompressed areas, whereas embossed wax-bonded areas serve as hydrophobic barriers laterally confining flow. Lab-made paper sheets first coated with a were hot-embossed tailor-made metal stamp. Both (e.g., grammage, fiber type) paper-extrinsic parameters force) studied...