microRNAs (miRNAs) are short 20- to 22-nucleotide noncoding RNAs that negatively regulate the expression of target genes at post-transcriptional level. The specific miRNAs and their roles in osteogenic differentiation human periodontal ligament stem cells (PDLSCs) exposed mechanical stretch remain unclear. Here, we found induced both differential miR-21 PDLSCs. Furthermore, identified activin receptor type IIB (ACVR2B) as a gene miR-21. Luciferase reporter assays showed interacts directly...

Human periodontal ligament stem cells (hPDLSCs) are mesenchymal (MSCs) derived from dental and craniofacial tissues that exhibit high potential for differentiation into osteoblasts. Recently, microRNAs (miRNAs) have been established to play important roles in MSC osteogenesis. In the current study, we report miR-21 was down-regulated osteogenically differentiated PDLSCs. Overexpression of significantly inhibited osteogenesis hPDLSC, whereas its inhibition demonstrated opposite effects....

Abstract Periodontal ligament stem cells (PDLSCs) are mesenchymal derived from dental tissues with multidirectional differentiation potential and excellent self-renewing ability. Recently, long noncoding RNAs (lncRNAs) have been shown to play important roles in MSC osteogenic differentiation. In this study, we found that taurine upregulated gene 1 (TUG1), an evolutionarily conserved widely present lncRNA was significantly osteogenically induced PDLSCs compared their undifferentiated...

Long non-coding RNAs (lncRNAs) play important roles in various physiological and pathophysiological processes. However, the effect of mechanical force on lncRNAs their role osteogenic differentiation periodontal ligament stem cells (PDLSCs) remains unclear. Here, we showed that expression lncRNA small nucleolar RNA host gene 8 (SNHG8) was steadily declined PDLSCs under force. This reduced SNHG8 promoted After knockdown by shRNA, osteogenic-related genes increased Regarding regulatory ability...

Circulating immune cells influence the efficacy of cancer therapy. This study aimed to investigate prognostic values different peripheral blood leukocyte (PBL) biomarkers in non-small lung (NSCLC) patients treated with chemoradiotherapy.An independent cohort 176 stage III NSCLC who were diagnosed at Shanghai Pulmonary Hospital and Zhejiang Cancer between April, 2010, September, 2018, had available pretreatment tests was enrolled. The all concurrent or sequential chemoradiotherapy according...

Occlusal force is an important stimulus for maintaining periodontal homeostasis. This attributed to the quality of human ligament fibroblasts (hPDLFs) that could transfer occlusal into biological signals modulating osteoblst differentiation. However, few studies investigated mechanism force‐induced osteodifferentiation hPDLFs. In our study, we used cyclic mechanical tension (CMT) at 10% elongation with 0.5 Hz mimic force, and explored its effects on osteogenesis Firstly, elevated expressions...

As an inflammatory disease, pre-eclampsia is correlated with elevation of pro-inflammatory cytokines and maternal endothelial dysfunction. Aspirin plays important role in the prevention therapy pre-eclampsia. Quercetin a bioflavonoid which has anti-oxidant reno-protective abilities. We aimed to figure out effects quercetin supplement aspirin on against Female pregnant Sprague-Dawley rats were divided into five groups according drug treatment. [1.5 mg/kg body weight (BW)] or (2 BW) treatment...

Flap Endonuclease 1 (FEN1) is a known oncogene in an array of cancers, but its role hepatocellular carcinoma (HCC) remains obscure. In this study, we report that FEN1 expression was elevated the Cancer Genome Atlas (TCGA) database which verified HCC tissue and hepatoma cell lines. Pearson correlation analysis indicated involved metastasis. We demonstrated silencing inhibits epithelial-mesenchymal transition (EMT), invasion migration vitro significantly suppressed tumor growth metastasis...

Abstract Objectives miR‐21 can promote osteoblast differentiation of periodontal ligament stem cells. However, the effect on bone remodelling in midpalatal suture is unclear. This study aimed to elucidate effects by expanding palatal sutures. Materials and methods deficient (miR‐21 −/− ) wild‐type (WT) mice were used establish animal models Micro‐CT, haematoxylin‐eosin (HE) staining, tartrate‐resistant acid phosphatase (TRAP) fluorescence labelling immunohistochemistry investigate function...

Background: Our previous study revealed that astragaloside IV (AS-IV) effectively improved gestational diabetes mellitus (GDM) by reducing hepatic gluconeogenesis. Due to the importance of placental oxidative stress, we further explored protective role AS-IV on stress in GDM. Methods: First, non-pregnant mice were orally administrated with evaluate its safety and effect. Then GDM administered for 20 days effect symptoms GDM, secretions inflammatory cytokines, as well toll-like receptor 4...

The glucose intolerance developed during pregnancy is called gestational diabetes mellitus (GDM). GDM has become a severe risk for the health of both mother and baby. Astragaloside IV (AS-IV) dominant active component in Astragalus membranaceus been reported to have anti-inflammation immune-regulation function. We aimed demonstrate function AS-IV therapy molecular mechanism this process. C57BL/KsJ-Lep db/+ female mice were used as model. mRNA levels relative genes research detected by...

Preeclampsia (PE) can cause serious health problems for pregnant women and their infants. Astragaloside IV has been shown to exert cardioprotective, anti-inflammatory, antioxidative effects on various disorders. We aimed study the of PE symptoms using an N G -nitro-l-arginine methyl ester (l-NAME)-induced rat model PE. The rats’ physiological features, including blood pressure, urine protein, serum soluble fms-like tyrosine kinase- 1 ( sFlt - 1)/placental growth factor (PlGF) ratio, weight...

Hepatocellular carcinoma (HCC) is a common yet deadly form of malignant cancer. However, the specific mechanisms involved in HCC diagnosis have not fully elucidated. Herein, we screened four publically available Gene Expression Omnibus (GEO) expression profiles (GSE14520, GSE29721, GSE45267 and GSE60502), used them to identify 409 differentially expressed genes (DEGs), including 142 267 up- down-regulated genes, respectively. The DAVID database was look for functionally enriched pathways...

Orthodontic force may lead to cell damage, circulatory disturbances, and vascular changes of the dental pulp, which make a hypoxic environment in pulp. In order maintain homeostasis hypoxia will inevitably induce defensive reaction. However, this is complex process regulated by numerous factors. study, we established an experimental animal model orthodontic tooth movement investigate effects mechanical on expression VEGF HIF-1 α Histological analysis pulp expressions proteins were examined....

Abstract Protein kinase-like endoplasmic reticulum kinase (PERK) is a type I transmembrane protein located in the (ER). The PERK-eukaryotic initiation factor 2α (eIF2α)-activating transcription 4 (ATF4) pathway has been proved to be involved osteoblast differentiation, but involvement of PERK-eIF2α-ATF4 signaling osteogenic differentiation human periodontal ligament stem cells (hPDLSCs) remained unclear. Therefore, aim this study was explore role PERK hPDLSCs and assess whether contributes...

Aspirin is a common drug for the treatment of pre-eclampsia. We aimed to explore whether quercetin as supplement aspirin could enhance therapeutic outcome in pre-eclampsia rat models. further evaluate nucleotide-binding oligomerization domain-like receptor family, pyrin domain containing 3 (NLRP3) inflammasome potential pre-eclampsia-related molecular mechanism, which can be affected by treatment.Rat models were established using an intravenous lipopolysaccharide injection after gestation....

Preeclampsia is a disorder of hypertension and proteinuria accompanied by abnormal inflammatory responses. Both aspirin quercetin possess anti-inflammatory anti-hypertensive properties. A low dose recommended for the prevention preeclampsia in patients with history. Whether can enhance effect on remains elusive.Female Sprague-Dawley pregnant rats were treated daily administration aspirin, quercetin, or combination subsequently received lipopolysaccharides (LPS) injection to induce...

The aims of the present study were: a) to investigate current state postpartum glucose screening in rural China; and b) explore factors influencing blood among women with prior GDM based on Andersen's behavioural model health service use.A multisite, cross-sectional design, conducted from November 2017 January 2018.A total 465 were included two county-level hospitals China. potential for model, including predisposing, enabling, need factors, collected by self-reported questionnaires....