A5081653994- Colorectal Cancer Treatments and Studies
- Radiomics and Machine Learning in Medical Imaging
- Cancer Treatment and Pharmacology
- Medical Imaging Techniques and Applications
- Lung Cancer Treatments and Mutations
- AI in cancer detection
- Chronic Kidney Disease and Diabetes
- Aortic aneurysm repair treatments
- Laser Applications in Dentistry and Medicine
- Organ Transplantation Techniques and Outcomes
- Cancer Genomics and Diagnostics
- Liver Disease and Transplantation
- Ion Channels and Receptors
- Metabolism, Diabetes, and Cancer
- Dialysis and Renal Disease Management
- Diet, Metabolism, and Disease
- Dermatologic Treatments and Research
- Cell Image Analysis Techniques
- Genetic Syndromes and Imprinting
- Microbial Metabolites in Food Biotechnology
- Photonic Crystal and Fiber Optics
- Biotin and Related Studies
- Renal Transplantation Outcomes and Treatments
- Anesthesia and Neurotoxicity Research
- Cystic Fibrosis Research Advances
University of Leeds
2006-2024
St James's University Hospital
2004-2023
Wellcome Trust
2011-2023
South Dakota State University
2023
Institute of Molecular Medicine
2010
University Teaching Hospital
2009
Leeds Teaching Hospitals NHS Trust
2004
University of Tennessee at Knoxville
2003
Renal Association
1988-1991
Ketohexokinase (KHK, also known as fructokinase) initiates the pathway through which most dietary fructose is metabolized. Very little about cellular localization of this enzyme. Alternatively spliced KHK-C and KHK-A mRNAs are known, but existence protein isoform has not been demonstrated in vivo. Using antibodies to KHK for immunohistochemistry Western blotting rodent tissues, including those from mouse knockouts, coupled with RT-PCR assays, we determined distribution splice variants. The...
Abstract Recent evidence suggests that the ion channel TRPA1 is implicated in lung adenocarcinoma (LUAD), where its role and mechanism of action remain unknown. We have previously established membrane receptor FGFR2 drives LUAD progression through aberrant protein–protein interactions mediated via C-terminal proline-rich motif. Here we report N-terminal ankyrin repeats directly bind to motif inducing constitutive activation receptor, thereby prompting metastasis. Furthermore, show upon...
PURPOSE High densities of tumor infiltrating CD3 and CD8 T-cells are associated with superior prognosis in colorectal cancer (CRC). Their value as predictors benefit from adjuvant chemotherapy is uncertain. PATIENTS AND METHODS Tumor tissue 868 patients the QUASAR trial (adjuvant fluorouracil/folinic acid v observation stage II/III CRC) was analyzed by immunohistochemistry. Pathologists, assisted artificial intelligence, calculated cell (cells/mm 2 ) core (CT) invasive margin (IM)....
Dietary fructose intake has dramatically increased over recent decades and is implicated in the high rates of obesity, hypertension, type 2 diabetes (metabolic syndrome) Western societies. The molecular determinants this epidemiologic correlation are incompletely defined, but high-flux catabolism initiated by ketohexokinase (Khk, fructokinase) believed to be important. Khk gene encodes two enzyme isoforms with distinctive substrate preferences, independent physiological roles which unclear....
Abstract Background Indoleamine 2,3-dioxygenase (IDO), the first step in kynurenine pathway (KP), is upregulated some cancers and represents an attractive therapeutic target given its role tumour immune evasion. However, recent failure of IDO inhibitor a late phase trial raises questions about this strategy. Methods Matched renal cell carcinoma (RCC) normal kidney tissues were subject to proteomic profiling. Tissue immunohistochemistry gene expression data used validate findings. Phenotypic...
Abdominal aortic aneurysm (AAA) is a focal dilatation progressing towards rupture. Non-invasive AAA-associated cell proliferation biomarkers are not yet established. We investigated the feasibility of radiotracer, fluorine-18-fluorothymidine ([
Tar spot caused by the fungus Phyllachora maydis Maubl. is a significant foliar disease of corn (Zea mays L.). Threatening production across Americas, this can reduce quality silage and grain yield (Rocco da Silva et al. 2021; Valle-Torres 2020). Lesions P. are usually black, glossy, raised stromata on leaf surface occasionally husk. (Liu 1973; Rocco 2021). Samples consistent with tar were collected between September October 2022 from 6 fields in Kansas, 23 Nebraska, South Dakota. One sample...
Abstract Digital microscopy produces high resolution digital images of pathology slides. Because no acceptable and effective control color reproduction exists in this domain, there is significant variability whole slide images. Guidance from international bodies regulators highlights the need for standardization. To address issue, we systematically measured analyzed spectra histopathological stains. This information was used to design a unique calibration utilizing real stains tissue‐like...
Investigations of glomerular anionic charge status in human renal biopsies have previously been restricted, by the techniques and markers used, to staining sites pre-embedded tissue. The introduction a novel marker, cationic colloidal gold, which demonstrates fixed hydrophilic resin (LR Gold)-embedded, ultrathin tissue sections, has now enabled be evaluated routine biopsy material. gold marker detects components express under different pH conditions. patterns showing minor pathology low...
Abstract Endoluminal surgery for the treatment of colorectal neoplasia is typically carried out using electrocautery tools which imply limited precision and risk harm through collateral thermal damage to adjacent healthy tissue. As a potential alternative, we present successful colonic epithelial laser ablation by means picosecond pulses. Laser studies performed in ex-vivo colon tissue result cavities with comparable thickness early stage cancers. The corresponding histology sections exhibit...
High tumor mRNA levels of the EGFR ligands amphiregulin (AREG) and epiregulin (EREG) are associated with anti-EGFR agent response in metastatic colorectal cancer (mCRC). However, ligand RNA assays have not been adopted into routine practice due to issues analytic precision practicality. We investigated whether AREG/EREG IHC could predict benefit from panitumumab.Artificial intelligence algorithms were developed assess 274 patients PICCOLO trial irinotecan or without panitumumab (Ir vs....
Abstract Transient Receptor Potential Canonical 5 (TRPC5) is a subunit of Ca 2+ -permeable non-selective cationic channel which negatively regulates adiponectin but not leptin in mice fed chow diet. Adiponectin major anti-inflammatory mediator and so we hypothesized an effect TRPC5 on the inflammatory condition atherosclerosis. Atherosclerosis was studied aorta ApoE −/− western-style Inhibition ion permeation achieved by conditional transgenic expression dominant negative pore mutant (DNT5)....
Abstract Ultrashort pulse lasers offer great promise for tissue resection with exceptional precision and minimal thermal damage. Surgery in the bowel requires high necrotic to avoid severe complications such as perforation. The deployment of ultrashort minimally invasive or endoscopic procedures has been hindered by lack suitable optical fibres peak powers. However, recent developments hollow core microstructured provide potential delivery pulses throughout body. In this study, analysis...
In electron microscopy, the post-embedding immunogold technique provides a high degree of resolution and possibility quantitation owing to intrinsic characteristics colloidal gold marker. Application this subcellular localization multiple antigens by differential labeling using markers different sizes, or double same primary antibody isotype with serial silver enhancement, has been reported. We have incorporated into modified procedure that produces excellent ultrastructural preservation,...
<p>Scatterplot: AREG vs EREG</p>
<p>Example AREG and EREG immunohistochemistry</p>