A5006005723- Prostate Cancer Treatment and Research
- Ubiquitin and proteasome pathways
- Cancer, Lipids, and Metabolism
- PI3K/AKT/mTOR signaling in cancer
- FOXO transcription factor regulation
- Cancer, Hypoxia, and Metabolism
- Epigenetics and DNA Methylation
- Cancer-related Molecular Pathways
- Protein Degradation and Inhibitors
- RNA modifications and cancer
- Histone Deacetylase Inhibitors Research
- Cancer-related gene regulation
- Estrogen and related hormone effects
- Advanced Breast Cancer Therapies
- Cancer-related molecular mechanisms research
- Protein Tyrosine Phosphatases
- Inflammatory mediators and NSAID effects
- Ocular Oncology and Treatments
- PARP inhibition in cancer therapy
- Chromatin Remodeling and Cancer
- DNA Repair Mechanisms
- Metabolomics and Mass Spectrometry Studies
- Cancer Immunotherapy and Biomarkers
- Neuroblastoma Research and Treatments
- Multiple Myeloma Research and Treatments
Mayo Clinic
2014-2023
Mayo Clinic in Florida
2020-2021
Mayo Clinic in Arizona
2004-2017
University of Minnesota Rochester
2015
Indiana University – Purdue University Indianapolis
2011-2014
Indiana University School of Medicine
2011-2014
University of Minnesota
2007-2014
Nanjing Normal University
2014
Virginia BioTechnology Research Park
2014
Masonic Cancer Center
2008-2014
Prostate cancer patients with regional lymph node involvement at radical prostatectomy often experience disease progression to other organs, the bone as predominant site. The transcription factor Runx2 plays an important role in formation and prostate cell migration, invasion, metastasis. Here we showed that forkhead box O (FOXO1) protein, a key downstream effector of tumor suppressor PTEN, inhibits transcriptional activity cells. This inhibition was enhanced by PTEN but diminished active...
Abstract Hepatocellular carcinoma (HCC) is one of the leading cause cancer death in world. Fructose-1,6-biphosphatase (FBP1), a rate-limiting enzyme gluconeogenesis, has been identified recently as tumor suppressor HCC and other types. In this study, we demonstrated that tripartite motif-containing protein 28 (TRIM28) binds directly to promotes FBP1 for ubiquitination degradation. MAGE-A3 MAGE-C2, which are known be overexpressed HCC, can enhance TRIM28-dependent degradation by forming...
Abstract Overexpression of the histone acetyltransferase p300 is implicated in proliferation and progression prostate cancer, but evidence a causal role lacking. In this study, we provide genetic that generic transcriptional coactivator functions as positive modifier tumorigenesis. mouse model PTEN deletion–induced ablation attenuated expression androgen receptor (AR). This finding was confirmed human cancer cells which abolished by RNA interference–mediated attenuation. These results were...
DNA-binding proteins, including transcription factors (TFs), play essential roles in various cellular processes and pathogenesis of diseases, deeming to be potential therapeutic targets. However, these proteins are generally considered undruggable as they lack an enzymatic catalytic site or a ligand-binding pocket. Proteolysis-targeting chimera (PROTAC) technology has been developed by engineering bifunctional molecule bring protein interest (POI) the proximity E3 ubiquitin ligase, thus...
Androgen receptor–positive prostate cancer (PCa) and estrogen luminal breast (BCa) are generally less responsive to immunotherapy compared with certain tumor types such as melanoma. However, the underlying mechanisms not fully elucidated. In this study, we found that FOXA1 overexpression inversely correlated interferon (IFN) signature antigen presentation gene expression in PCa BCa patients. bound STAT2 DNA-binding domain suppressed activity, IFN signaling expression, immune response...
Abstract Retinoblastoma (RB) protein can exert tumor suppressor functions even when it becomes phosphorylated. It is thus essential to understand how phosphorylated RB (p-RB) expression and function are regulated. Here, we demonstrated that RING finger domain TRIM28 bound promoted ubiquitination degradation of CDK4/6-phosphorylated protein. SETDB1, a known binding partner, protected p-RB from through the methylated by its Tudor independent methyltransferase activity. SETDB1 was found be...
The tumor suppressor gene <i>PTEN</i>(<i>MMAC1/TEP1</i>) is lost frequently in advanced prostate cancer (PCa). However, the function of PTEN tumorigenesis not understood fully. In this study, we demonstrate that expression Bcl-2 tumors correlates with loss protein. This finding was verified by studies PCa cell lines DU145, PC-3, LNCaP, and an androgen-refractory subline LNCaP. Transient transfection into PTEN-null cells resulted decreased levels mRNA These effects appear to be mediated at...
Abstract Hepatocellular carcinoma (HCC) is one of the most commonly diagnosed cancers in world. Elevated glucose metabolism availability oxygen, a phenomenon called Warburg effect, important for cancer cell growth. Fructose-1,6-bisphosphatase (FBP1) rate-limiting enzyme gluconeogenesis and frequently lost various types cancer. Here, we demonstrated that expression FBP1 was downregulated HCC patient specimens decreased associated with poor prognosis. Low correlated high levels histone...
Abstract Dysregulation of the MAPK pathway correlates with progression pancreatic ductal adenocarcinoma (PDAC) progression. IQ motif containing GTPase-activating protein 1 (IQGAP1) is a scaffold that directly regulates activation RAF, MEK, and ERK. Fructose-1,6-bisphosphatase (FBP1), key enzyme in gluconeogenesis, transcriptionally downregulated various cancers, including PDAC. Here, we demonstrate FBP1 acts as negative modulator IQGAP1–MAPK signaling axis PDAC cells. binding to WW domain...
Article26 September 2019Open Access Source DataTransparent process The novel BET-CBP/p300 dual inhibitor NEO2734 is active in SPOP mutant and wild-type prostate cancer Yuqian Yan Department of Biochemistry Molecular Biology, Mayo Clinic College Medicine Science, Rochester, MN, USA Search for more papers by this author Jian Ma Urology, Fudan University Shanghai Cancer Center, Shanghai, China Oncology, Medical College, University, Dejie Wang Dong Lin Experimental Therapeutics, BC Research...
Abstract Purpose: Intratumoral androgen synthesis (IAS) is a key mechanism promoting receptor (AR) reactivation and antiandrogen resistance in castration-resistant prostate cancer (CRPC). However, signaling pathways driving aberrant IAS remain poorly understood. Experimental Design: The effect of components the AKT-RUNX2-osteocalcin (OCN)–GPRC6A–CREB axis on expression steroidogenesis genes CYP11A1 CYP17A1 testosterone level were examined PTEN-null human cell lines. Pten knockout mice used...
Abstract Purpose: Deletions or mutations in PTEN and TP53 tumor suppressor genes have been linked to lineage plasticity therapy-resistant prostate cancer. Fusion-driven overexpression of the oncogenic transcription factor ERG is observed approximately 50% all cancers, many which also harbor alterations. However, role PTEN/TP53–altered tumors unclear. Understanding collective effect multiple within one essential combat plasticity-driven therapy resistance. Experimental Design: We generated a...
Abstract The tumor-suppressor protein RB acts as a transcription repressor via interaction of its pocket domain with an LXCXE motif in histone deacetylase (HDAC) proteins such HDAC1. Here, we demonstrate that HDAC5 deficient for the interacts both RB-N (via FXXXV motif) and RB-C segments, interactions are diminished by phosphorylation serine-249/threonine-252 threonine-821. was frequently downregulated or deleted human cancers prostate cancer. Loss increased H3 lysine 27 acetylation...
N6-methyladenosine (m6A) of mRNAs modulated by the METTL3-METTL14-WTAP-RBM15 methyltransferase complex and m6A demethylases such as FTO play important roles in regulating mRNA stability, splicing, translation. Here, we demonstrate that FTO-IT1 long noncoding RNA (lncRNA) was upregulated positively correlated with poor survival patients wild-type p53-expressing prostate cancer (PCa). RIP-seq analysis revealed knockout increased methylation a subset p53 transcriptional target genes (e.g., FAS,...
E26 transformation-specific transcription factor ERG is aberrantly overexpressed in approximately 50% of all human prostate cancer due to
De novo fatty acid (FA) synthesis is required for prostate cancer (PCa) survival and progression. As a key enzyme FA synthase (FASN) often overexpressed in human cancers its expression correlates with worse prognosis poor survival. P300 an acetyltransferase that acts as transcription co-activator. Increasing evidence suggests major PCa promoter, although the underlying mechanism remains poorly understood. Here, we demonstrated binds to increases histone H3 lysine 27 acetylation (H3K27Ac)...
Research Article9 March 2018Open Access Source DataTransparent process Dual inhibition of AKT-mTOR and AR signaling by targeting HDAC3 in PTEN- or SPOP-mutated prostate cancer Yuqian Yan Department Gastroenterology, Jiangxi Institute Gastroenterology Hepatology, First Affiliated Hospital Nanchang University, Nanchang, Jiangxi, China Biochemistry Molecular Biology, Mayo Clinic College Medicine, Rochester, MN, USA Search for more papers this author Jian An Yinhui Yang Urology, The Fourth...
Abstract Mutations in SPOP E3 ligase gene are reportedly associated with genome-wide DNA hypermethylation prostate cancer (PCa) although the underlying mechanisms remain elusive. Here, we demonstrate that binds and promotes polyubiquitination degradation of histone methyltransferase DNMT interactor GLP. mutation induces stabilization GLP its partner protein G9a aberrant upregulation global cultured PCa cells primary specimens. Genome-wide methylome analysis shows a subset tumor suppressor...
Approximately 50% of prostate cancer (PCa) patients harbor fusions involving the TMPRSS2 and ERG genes. Despite this, tailored therapies targeting fused gene, tERG, remain undeveloped. Our study analyzed biopsy samples from two clinical trials assessing efficacies androgen receptor (AR) signaling inhibitors (ARSIs). The results revealed that tERG promotes resistance to ARSIs is associated with elevated levels glucocorticoid (GR). Subsequent assays showed GR directly interacts alleviates...
// Changping Wu 1, * , Xin Jin 2, Jing Yang 2 Yinhui Yundong He Liya Ding Yunqian Pan Shuai Chen 5 Jingting Jiang 1 Haojie Huang 3, 4 Department of Tumor Biological Treatment, The Third Affiliated Hospital Soochow University, Changzhou 213003, China Biochemistry and Molecular Biology, Mayo Clinic College Medicine, Rochester, MN 55905, USA 3 Urology, Cancer Center, Sun Yat-sen University State Key Laboratory Oncology in South China, Collaborative Innovation Center Guangzhou 510060, These...