A5101788197- Cancer Genomics and Diagnostics
- CRISPR and Genetic Engineering
- Viral Infectious Diseases and Gene Expression in Insects
- Genomics and Chromatin Dynamics
- Virus-based gene therapy research
- DNA Repair Mechanisms
- Glioma Diagnosis and Treatment
- RNA modifications and cancer
- Epigenetics and DNA Methylation
- bioluminescence and chemiluminescence research
- RNA Research and Splicing
- Retinal Development and Disorders
- Spine and Intervertebral Disc Pathology
- Prenatal Screening and Diagnostics
- Cancer Cells and Metastasis
- Genetic factors in colorectal cancer
- Heat shock proteins research
- Computational Drug Discovery Methods
- Cancer Immunotherapy and Biomarkers
- Spinal Fractures and Fixation Techniques
- Ferroptosis and cancer prognosis
- ATP Synthase and ATPases Research
- Acute Myeloid Leukemia Research
- Poxvirus research and outbreaks
- Bacterial Genetics and Biotechnology
University of Pennsylvania
2019-2025
Philadelphia University
2023
Massachusetts Institute of Technology
2021-2023
St. John's School
2022
The University of Melbourne
2022
Guangzhou Medical University
2020
Rockefeller University
2017-2019
University of California, San Diego
2015-2019
Neurological Surgery
2019
London Health Sciences Centre
2019
DNA mismatch repair deficiency (MMRd) is associated with a high tumor mutational burden (TMB) and sensitivity to immune checkpoint blockade (ICB) therapy. Nevertheless, most MMRd tumors do not durably respond ICB critical questions remain about immunosurveillance TMB in these tumors. In the present study, we developed autochthonous mouse models of lung colon cancer. Surprisingly, did display increased T cell infiltration or response, which showed be result substantial intratumor...
Significance Noninvasive prenatal testing (NIPT) using sequencing of fetal cell-free DNA from maternal plasma has allowed accurate diagnosis aneuploidy without requirement an invasive procedure; this approach gained increasing clinical acceptance. In manuscript, we evaluate whether NIPT semiconductor platform (SSP) could reliably detect subchromosomal deletions/duplications in women carrying a high-risk fetus. We show that detection abnormalities is viable extension SSP. Given the recent...
Replicative helicases in all cell types are hexameric rings that unwind DNA by steric exclusion which the helicase encircles tracking strand only and excludes other from ring. This mode of translocation allows to bypass blocks on is excluded central channel. Unlike replicative helicases, eukaryotic CMG partially duplex at a forked junction stopped block non-tracking (lagging) strand. report demonstrates Mcm10, an essential replication protein unique eukaryotes, binds greatly stimulates its...
The current view is that eukaryotic replisomes are independent. Here we show Ctf4 tightly dimerizes CMG helicase, with an extensive interface involving Psf2, Cdc45, and Sld5. Interestingly, binds only one Pol α-primase. Thus, may have evolved as a trimer to organize two helicases α-primase into replication factory. In the 2CMG–Ctf43–1Pol factory model, CMGs nearly face each other, placing lagging strands toward center leading out sides. single centrally located prime both sister replisomes....
The tyrosine kinase ACK1, a critical signal transducer regulating survival of hormone-refractory cancers, is an important therapeutic target, for which there are no selective inhibitors in clinical trials to date. This work reports the discovery novel and potent ACK1 (also known as TNK2) using innovative fragment-based approach. Focused libraries were designed synthesized by selecting fragments from reported ACK create hybrid structures mix match process. library was screened enzyme-linked...
Abstract The ability to identify a specific type of leukemia using minimally invasive biopsies holds great promise improve the diagnosis, treatment selection, and prognosis prediction patients. Using genome-wide methylation profiling machine learning methods, we investigated utility CpG status differentiate blood from patients with acute lymphocytic (ALL) or myelogenous (AML) normal blood. We established panel that can distinguish ALL AML as well high sensitivity specificity. then developed...
This is a case report.To present novel of lumbosacral dislocation and its surgical management.Complete dislocations are rare injuries that ensue as result high-energy trauma. Anatomic stabilization these can be challenging often involves open fixation arthrodesis.We the 22-year-old male who was involved in high-velocity motorcycle accident with neurological deficit lower extremities. Radiographic analysis demonstrated complete lateral L5 vertebral body over sacrum. The patient surgically...
Non‐invasive prenatal testing (NIPT) using sequencing of fetal cell‐free DNA from maternal plasma has enabled accurate diagnosis aneuploidy and become increasingly accepted in clinical practice. We investigated whether NIPT semiconductor (SSP) could reliably detect subchromosomal deletions/duplications women carrying high‐risk fetuses. first demonstrated that increasing concentration abnormal depth improved detection. Subsequently, we analyzed 1456 pregnant to develop a method for estimating...
We showed that WT adeno-associated virus serotype 2 (AAV2) genome devoid of a conventional polyadenylation [poly(A)] signal underwent complete replication, encapsidation and progeny virion production in the presence adenovirus. The infectivity was also retained. Using recombinant AAV2 vectors human growth hormone poly(A) signal, we demonstrated subset mRNA transcripts contained inverted terminal repeat (ITR) sequence at 3′ end, which designated ITR RNA (ITRR). Furthermore, AAV replication...
Lung adenocarcinoma (LUAD) is a significant subtype of lung cancer, and understanding the roles tumor suppressor genes in LUAD remains crucial. In this study, we aimed to identify experimentally validate potential selected from online databases. By analyzing TCGA cancer database, identified exhibiting differential expression between normal tissues LUAD, particularly focusing on with lower levels tumors associated shortened survival periods. Subsequently, UNC5B, DAB2IP, SEMA3F, PPM1L, AXIN2...
Abstract Glioblastoma (GBM), a deadly brain cancer, infiltrates the and can be synaptically innervated by neurons. Synaptic inputs onto GBM cells identified so far are largely short-range glutamatergic in nature. The extent of integration into brain-wide neuronal circuitry is therefore not well understood. We report application transsynaptic viral tracing approaches to study connectome GBM. applied rabies virus-mediated retrograde herpes simplex virus (HSV)-mediated anterograde characterize...
ABSTRACT The current view is that eukaryotic replisomes are independent. Here we show Ctf4 tightly dimerizes CMG helicase, with an extensive interface involving Psf2, Cdc45, and Sld5. Interestingly, binds only one Pol α-primase. Thus, may have evolved as a trimer to organize two helicases α-primase into replication factory. In the 2CMG-Ctf4 3 -1Pol factory model, CMGs nearly face each other, placing lagging strands toward center leading out sides. single centrally located prime both sister...
We previously observed that purified recombinant glucocorticoid receptor (GR) protein specifically binds to the D-sequence within inverted terminal repeat of AAV2 genome because shares partial homology consensus half-site receptor-binding element (GRE). The is an essential core element, which has been reported be sufficient mediate GR signaling. Substitution with authentic GRE further increased vector-mediated transgene expression. Based on these observations, we systemically examined...