A5042005973- Respiratory viral infections research
- Metabolism and Genetic Disorders
- Asthma and respiratory diseases
- Pulmonary Hypertension Research and Treatments
- Neonatal Respiratory Health Research
- Congenital Heart Disease Studies
- Tracheal and airway disorders
- Porphyrin Metabolism and Disorders
- Genomic variations and chromosomal abnormalities
- Pediatric health and respiratory diseases
- Viral gastroenteritis research and epidemiology
- Congenital Diaphragmatic Hernia Studies
- Biochemical and Molecular Research
- Cutaneous lymphoproliferative disorders research
- Skin and Cellular Biology Research
- Dermatological and Skeletal Disorders
- Autoimmune Bullous Skin Diseases
- Pancreatitis Pathology and Treatment
- Neonatal Health and Biochemistry
- Renal Diseases and Glomerulopathies
- Interstitial Lung Diseases and Idiopathic Pulmonary Fibrosis
- Mitochondrial Function and Pathology
- Cardiac Arrhythmias and Treatments
- Eosinophilic Disorders and Syndromes
- Pleural and Pulmonary Diseases
University of Toronto
2011-2021
Hospital for Sick Children
2014-2021
SickKids Foundation
2015-2020
University of British Columbia
1999-2016
St. Michael's Hospital
2010-2016
McMaster University
2016
Université de Montréal
2016
Western University
2016
Dalhousie University
2016
Memorial University of Newfoundland
2016
It is not known if a surgical lung biopsy necessary in all patients for the diagnosis of idiopathic pulmonary fibrosis (IPF). We conducted blinded, prospective study at eight referring centers. Initially, cases were evaluated by clinical history and examination, transbronchial biopsy, high-resolution computed tomography scans. Pulmonologists centers then assessed their certainty IPF provided an overall diagnosis, before biopsy. The biopsies reviewed pathology core 54 91 received pathologic...
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ABSTRACT Respiratory viruses exert a heavy toll of morbidity and mortality worldwide. Despite this burden there are few specific treatments available for respiratory virus infections. Since many utilize host cell enzymatic machinery such as protein kinases replication, we determined whether pharmacological inhibition could, in principle, be used broad antiviral strategy common human A panel green fluorescent (GFP)-expressing recombinant viruses, including an isolate H1N1 influenza...
Primary ciliary dyskinesia (PCD) is a heterogeneous genetic condition. European and North American diagnostic guidelines recommend transmission electron microscopy (TEM) as one of combination tests to confirm diagnosis. However, there no definition what constitutes defect or consensus on reporting terminology. The aim this project was provide an internationally agreed ultrastructural classification for PCD diagnosis by TEM. A guideline developed experts representing 18 centres in 14...
The tachykinin neuropeptides substance P and neurokinin (NK) A have been postulated to participate in the inflammatory reaction airways of smokers asthmatics. We examined hypothesis that expression one or more three cloned receptors (NK1, NK2, NK3) is increased airway disorders, which could result augmentation effect released neuropeptides. NK1 receptor NK2 but not NK3-receptor mRNA were detected by ribonuclease protection assay RNA from both cartilaginous membranous bronchi subpleural lung....
Respiratory diseases are the most frequent chronic illnesses in babies and children. Although a vigorous innate immune system is critical for maintaining lung health, balanced response essential to minimize damaging inflammation. We investigated functional clinical impact of human genetic variants promoter NFKBIA, which encodes IκBα, major negative regulator NF-κB. In this study, we quantified NFKBIA polymorphisms (rs3138053, rs2233406, rs2233409) on promoter-driven protein expression,...
Abstract Background It is hypothesised that complex interactions between genetic and environmental factors give rise to allergy asthma in childhood. The C anadian H ealthy I nfant L ongitudinal D evelopment ( CHILD ) study was designed explore these factors. Methods a longitudinal, general population birth cohort following infants from mid‐pregnancy age 5 years. Over this time period, biological samples, questionnaires, clinical measures data are collected. Results A total of 3624 families...
Enteropathogenic Escherichia coli (EPEC) belongs to a family of related bacterial pathogens, including enterohemorrhagic (EHEC) O157:H7 and other human animal diarrheagenic pathogens that form attaching effacing (A/E) lesions on host epithelial surfaces. Bacterial secreted Esp proteins type III secretion system are conserved among these trigger cell signal transduction pathways cytoskeletal rearrangements, mediate intimate adherence surfaces in vitro. However, their role pathogenesis is...
Oncogenic osteomalacia is a syndrome characterized by phosphaturia, hypophosphatemia, reduced vitamin D levels, and osteomalacia. The cause not known, but all patients have had tumor; usually of mesenchymal origin. Removal the tumor reverses metabolic abnormalities. We report patient with osteomalacia, severe elevated alkaline phosphatase, low 1,25-dihydroxyvitamin D3, phosphaturia. A was identified in infratemporal fossa. removed, biochemical abnormalities resolved over subsequent 8 months....
The involvement of Epstein-Barr virus (EBV) in the pathogenesis lymphocytic interstitial pneumonia (LIP) was investigated using an situ hybridization technique. Archival lung tissue samples from 14 patients (six men and eight women with a mean age 56 ± 3 yr) whom diagnosis LIP had been previously established were retrospectively examined compared 10 four 58 idiopathic pulmonary fibrosis (IPF) who served as control subjects. In LIP, immunophenotype infiltrate determined by monoclonal...
Medical research to improve health care faces a major problem in the relatively limited availability of adequately annotated and collected biospecimens. This limitation is creating growing gap between pace scientific advances successful exploitation this knowledge. Biobanks are an important conduit for transfer biospecimens (tissues, blood, body fluids) related data research. They have evolved outside historical source tissue biospecimens, clinical pathology archives. Research biobanks...
The contribution of respiratory viral infections to the onset asthma and atopy is controversial. In "high risk" children (n = 455) born into asthmatic/atopic families, we determined relationship exposures common viruses concomitant symptoms, subsequent possible at ages 1 2 years. Frozen nasal specimens, obtained when were weeks, 4, 8, 12 months old, underwent reverse transcription-polymerase chain reaction (RT-PCR) testing for parainfluenza virus (PIV), syncytial (RSV), picornavirus...
respiratory viral infections account for a considerable proportion of pediatric emergency room visits. Illnesses range in severity from mild upper tract to serious lower (LRTI). The relationship between load and specific viruses clinical diagnosis made by physicians this setting is poorly understood.we applied real-time, quantitative polymerase chain reaction (qPCR) panel 13 common 195 frozen, archival nasopharyngeal aspirate specimens obtained symptomatic children ≤ 4 months age presenting...
Nucleolin is an essential cellular receptor to human respiratory syncytial virus (RSV). Pharmacological targeting of the nucleolin RNA binding domain RBD1,2 can inhibit RSV infections in vitro and vivo; however, site(s) on which interact with are not known. We undertook a series experiments designed to: document RSV-nucleolin co-localization surface polarized MDCK cells using immunogold electron microscopy, identify domains that physically biochemical methods determine their biological...
Hypersensitivity pneumonitis (HP), a lung disease with "flulike" symptoms, results from repeated exposures to well defined, nonpathogenic antigens. This study examined whether respiratory viruses are present in the lower airways, likely site of hypersensitivity reaction, patients HP. The polymerase chain reaction (PCR) method was used test for 10 common bronchoalveolar lavage (BAL) cells obtained acute HP and unexposed healthy volunteers. Immunocytochemistry subsequently localize viral...