A5101656642- Orthopaedic implants and arthroplasty
- Orthopedic Infections and Treatments
- Total Knee Arthroplasty Outcomes
- Bacteriophages and microbial interactions
- Microbial infections and disease research
- Tryptophan and brain disorders
- Gut microbiota and health
- Antibiotic Resistance in Bacteria
- Hip disorders and treatments
- Antimicrobial Peptides and Activities
- Bacterial biofilms and quorum sensing
- Hip and Femur Fractures
- Pneumonia and Respiratory Infections
- Stress Responses and Cortisol
- Biochemical and Structural Characterization
- Knee injuries and reconstruction techniques
- Hearing, Cochlea, Tinnitus, Genetics
- Bone and Joint Diseases
- Nerve injury and regeneration
- Bacterial Infections and Vaccines
- Nerve Injury and Rehabilitation
- Cardiac, Anesthesia and Surgical Outcomes
- Immune Response and Inflammation
- vaccines and immunoinformatics approaches
- Shoulder Injury and Treatment
Ruijin Hospital
2016-2025
Shanghai Jiao Tong University
2016-2025
Guangdong Academy of Medical Sciences
2018-2024
Guangdong Provincial People's Hospital
2019-2024
Chengdu University
2024
Fujian Medical University
2022-2024
Southern Medical University
2022-2024
Shandong Normal University
2022-2023
Zaozhuang University
2023
Institute of Art
2023
Little is known regarding differences in the gut microbiomes of rheumatoid arthritis (RA) patients and healthy cohorts China. This study aimed to identify fecal 66 Chinese with RA 60 controls. The V3-V4 variable regions bacterial 16S rRNA genes were sequenced Illumina system define composition. alpha-diversity index microbiome was significantly lower than that control group. genera Bacteroides (p=0.02202) Escherichia-Shigella (p=0.03137) more abundant patients. In contrast, Lactobacillus...
Carbapenem-resistant Escherichia coli (CREC) poses a severe global public health risk. This study reveals the worldwide geographic spreading patterns and spatiotemporal distribution characteristics of resistance genes in 7918 CREC isolates belonging to 497 sequence types (ST) originating from 75 countries. In last decade, there has been transition prevailing STs highly virulent ST131 ST38 higher antibiotic-resistant ST410 ST167. The rise multi-drug resistant strains carrying plasmids with...
The increasing prevalence of infections caused by multidrug-resistant Klebsiella pneumoniae necessitates the development alternative therapies. Here, we isolated, characterized, and sequenced a K. bacteriophage (SH-KP152226) that specifically infects lyses capsular type K47. phage SH-KP152226 contains genome 41,420 bp encodes 48 predicted proteins. Among these proteins, Dep42, gene product ORF42, is putative tail fiber protein hypothetically possesses depolymerase activity. We demonstrated...
This study explored the relationship between teacher job stress and burnout using a sample of 558 primary secondary school teachers, who were administered with scale, work-family conflict questionnaire, general self-efficacy scale. The results showed that: (1) had significant predictive effect on burnout; (2) played mediating role in (3) was found to play moderating burnout. However, this indirect stronger for teachers high self-efficacy, which means that protective effects limited. These...
With increasing attention paid to the concept of microbiota-gut-brain axis, mounting evidence reveals that gut microbiota is involved in a variety neurological and psychiatric diseases. However, changes major depressive disorder (MDD) patients their association with disease mechanisms remain undefined. Fifty MDD sixty healthy controls were recruited from Shanghai Healthy Mental Center, China. Fecal samples collected, compositional characteristics intestinal flora determined by MiSeq...
Abstract Staphylococcus phages of the Myoviridae family have a wide host range and potential applications in phage therapy. In this report, safety assessments these were conducted based on their complete genome sequences. The genomes analyzed, Open Reading Frame (ORFs) compared with pool virulence antibiotic resistance genes using BLAST algorithm. addition, lifestyle (virulent or temperate) was also confirmed PHACTS. results showed that all lytic did not contain bioinformatic analyses,...
Schizophrenia is a serious mental illness affecting approximately 20 million individuals globally. Both genetic and environmental factors contribute to the illness. If left undiagnosed untreated, schizophrenia results in impaired social function, repeated hospital admissions, reduced quality of life decreased expectancy. Clinical diagnosis largely relies on subjective evidence, including self-reported experiences, reported behavioural abnormalities followed by psychiatric evaluation. In...
Insights into the interaction between phages and their bacterial hosts are crucial for development of phage therapy. However, only one study has investigated global gene expression Clostridioides (formerly Clostridium) difficile carrying prophage, transcriptional reprogramming during lytic infection not been studied. Here, we presented isolation, propagation, characterization a newly discovered 35,109-bp phage, JD032, transcriptomes both JD032 C. ribotype 078 (RT078) strain TW11 infection....
Studies suggest that biological sex influences susceptibility to kidney diseases with males demonstrating greater risk for developing ischemic acute injury (AKI). Sex-related differences in mitochondrial function and homeostasis exist, likely contributing sexual dimorphism injury, but the mechanisms are not well characterized. Our observations reveal lower baseline expression of Sirtuin-3 (Sirt3, a major acetyltransferase) kidneys male mice versus females. We tested hypothesis differential...
Migratory birds play a pivotal role in the global dissemination of antimicrobial resistance genes (ARGs), with shorebirds relying on coastal wetlands during their long-distance migrations, environments often contaminated and conducive to ARG transmission. However, systematic investigations into (AMR) remain scarce. During spring autumn 2023, we collected 893 throat cloacal swabs from 480 shorebirds, representing 28 species, at Chongming Dongtan, critical stopover along East...
The implementation of phage therapy is re-emerging with the increase in widespread antibiotic-resistant bacteria. Staphylococcus JD007 was characterized and its complete genome sequence analysed. classified as belonging to Myoviridae family based on morphology, observed by transmission electron microscopy. Its lytic activity stable between pH 5–11 below 42 °C; moreover, an absorbance curve showed that nearly 90% viral particles had adsorbed host after a 20 min co-incubation. size 141,836 bp,...
Abstract Background Avascular necrosis of the femoral head (ANFH) is a severely disabling disease hip. Several clinical trials have shown promising outcomes on use mesenchymal stem cells for treatment ANFH, but long-term assessments are lacking. Previously, we reported 2-year follow-up results prospective, double-blinded, randomized, controlled study autologous bone marrow buffy coat grafting combined with core decompression in patients ANFH. Here, report 10-year this study. Methods We...
Carbapenem-resistant Klebsiella pneumoniae (CRKP), a pathogen that causes severe nosocomial infections and yields high mortality rate, poses serious threat to global public health due its antimicrobial resistance. Bacteriophages encode polysaccharide-degrading enzymes referred as depolymerases cleave the capsular polysaccharide (CPS), one of main virulence factors K. pneumoniae. In this study, we identified characterized new capsule depolymerase K19-Dpo41 from bacteriophage SH-KP156570. Our...
Transthoracic echocardiography (TTE) is a common cardiac screening test before hip fracture surgery. However, the general TTE delays surgery, so it would be meaningful if we could simplify by only assessing abnormality specifically. Therefore, aimed to establish most clinically relevant comparing predictive value of each major in postoperative complications and mortality elderly patients.From January 2014 2019, medical records all surgically treated patients (>65 years) with were analyzed....
Methicillin-resistant Staphylococcus aureus-related infections have become a serious problem worldwide. Bacteriophage therapy is an alternative approach against this threat. S. aureus phage JD007, which belongs to the Myoviridae family according transmission electron microscopic imaging, could lyse nearly 30% of strains from Ruijin Hospital, Shanghai, China, and was isolated chicken feces in China. The complete genome showed that JD007 linear, double-stranded DNA 141,836 bp length with GC...
Here, we present the first report of one suspected dead case and two confirmed rapid-onset fatal infections caused by a newly emerging hypervirulent Klebsiella pneumoniae ST86 strain serotype K2. The three cases occurred in surgery ward during 2013 Shanghai, China. A combination multilocus sequence typing, pulsed-field gel electrophoresis, phenotypic PCR tests for detecting virulence factors (VFs) was used to identify isolates as K2 strains with common VFs, including aerobactin rmpA....
Abstract Background Treatment of avascular necrosis the femoral head (ANFH) in young patients remains a clinical challenge. A current controversy is whether hip-preserving surgery results better outcomes. The adverse effects are associated with fill material for necrotic areas. This study aims to evaluate early autologous bone marrow buffy coat (BBC) and angioconductive bioceramic rod (ABR) grafting advanced core decompression (ACD) on ANFH. Methods Forty-four (57 hips) ANFH from 2015 2020...
Background Pertussis (whooping cough) caused by Bordetella pertussis (B.p), continues to be a serious public health threat. Vaccination is the most economical and effective strategy for preventing controlling pertussis. However, few systematic investigations of actual human immune responses vaccines have been performed. Therefore, we utilized combination two-dimensional electrophoresis (2-DE), immunoblotting, mass spectrometry reveal entire antigenic proteome whole-cell vaccine (WCV)...