A5082259375- Cancer Cells and Metastasis
- Hormonal and reproductive studies
- Immunotherapy and Immune Responses
- Cancer, Lipids, and Metabolism
- Prostate Cancer Treatment and Research
- Plant biochemistry and biosynthesis
- Head and Neck Cancer Studies
- Education, Psychology, and Social Research
- Lipoproteins and Cardiovascular Health
- Cancer-related Molecular Pathways
- RNA modifications and cancer
- RNA Research and Splicing
- Ubiquitin and proteasome pathways
- Sexual Differentiation and Disorders
- dental development and anomalies
- Angiogenesis and VEGF in Cancer
- Research on Leishmaniasis Studies
- MRI in cancer diagnosis
- Cancer Immunotherapy and Biomarkers
- Integrated Circuits and Semiconductor Failure Analysis
- Bladder and Urothelial Cancer Treatments
- Wound Healing and Treatments
- Trypanosoma species research and implications
- Bartonella species infections research
- Fatty Acid Research and Health
Czech Academy of Sciences, Institute of Molecular Genetics
2014-2025
Czech Academy of Sciences
2009-2025
Charles University
2007-2009
University Hospital Kralovske Vinohrady
2009
Nodular melanoma is one of the most life threatening tumors with still poor therapeutic outcome. Similarly to other tumors, permissive microenvironment essential for progression. Features this are arising from molecular crosstalk between cells (MC) and surrounding cell populations in context skin tissue. Here, we study effect on human primary keratinocytes (HPK). Presence MC as an important modulator tumor compare it nonmalignant lowly differentiated also originating neural crest (NCSC)....
Tumor stroma is an active part influencing the biological properties of malignancies via molecular cross-talk. Cancer-associated fibroblasts play a significant role in this interaction. These cells frequently express smooth muscle actin and can be classified as myofibroblasts. The adhesion/growth-regulatory lectin galectin-1 effector for their generation. In our study, we set presence actin-positive cancer-associated relation to endogenous vivo competitor (galectin-3). squamous cell...
Abstract Background information Considering an analogy between wound healing and tumour progression, we studied chemokine cytokine transcription expression in normal fibroblasts by co‐culture situ . Results Whole‐genome transcriptome profiling revealed strong upregulation for the interleukin (IL)‐6, IL‐8 CXCL‐1 vitro co‐cultures of with either or malignant epithelial cells compared to fibroblast cultures. The same ILs/chemokines were distinctly upregulated clinical samples squamous cell...
Abstract Neurodegeneration is a common hallmark of individuals with hereditary defects in DNA single-strand break repair; process regulated by poly(ADP-ribose) metabolism. Recently, mutations the ARH3 (ADPRHL2) hydrolase that removes ADP-ribose from proteins have been associated neurodegenerative disease. Here, we show -mutated patient cells accumulate mono(ADP-ribose) scars on core histones are molecular memory recently repaired breaks. We demonstrate chromatin result reduced endogenous...
The incidence of cutaneous malignant melanoma is increasing worldwide. While the treatment initial stages disease simple, advanced frequently remains fatal despite novel therapeutic options . This requires identification targets in melanoma. Similarly to other types tumours, cancer microenvironment plays a prominent role and determines biological properties Importantly, cell-produced exosomes represent an important tool intercellular communication within this ecosystem. We have focused on...
Abstract Mutations in BRAT1 , encoding BRCA1-associated ATM activator 1, have been associated with neurodevelopmental and neurodegenerative disorders characterized by heterogeneous phenotypes varying levels of clinical severity. However, the underlying molecular mechanisms disease pathology remain poorly understood. Here, we show that tightly interacts INTS9/INTS11 subunits Integrator complex processes 3’ ends various noncoding RNAs pre-mRNAs. We find functions are disrupted deletion. In...
Statin treatment of hypercholesterolemia is accompanied also with depletion the mevalonate intermediates, including farnesyl pyrophosphate (FPP) and geranylgeranyl (GGPP) necessary for proper function small GTPases. These include Ras proteins, prevalently mutated in pancreatic cancer. In our study, we evaluated effect three key intermediates pathway on GFP-K-Ras protein localization gene expression profile cancer cells after exposure to individual statins. effects were tested MiaPaCa-2 human...
1. Wistar rats were fed for three successive generations on a semi-purified diet, in which the fat was provided by butter, sunflower oil, rapeseed oil or hydrogenated vegetable fat, differing content of cis,cis-18:2 and trans-18:1 fatty acids. Effects these fats composition adipose tissue reproductive performance studied. Fatty acids analysed using high-performance liquid chromatography. 2. The acid pattern closely related to dietary and, established first generation, did not change...
The nonsyndromic cleft is one of the most frequent congenital defects in humans. Clinical data demonstrated improved and almost scarless neonatal healing reparative surgery. Based on our previous results crosstalk between fibroblasts adult keratinocytes, present study focused characterization prepared from lip tissue samples neonates older children, compared them with isolated normal skin (face breast) scars. Although subtle variances expression profiles children were observed, two groups...
Arthrospira platensis, a blue-green alga, is popular nutraceutical substance having potent antioxidant properties with potential anti-carcinogenic activities. The aim of our study was to assess the possible anti-angiogenic effects A platensis in an experimental model pancreatic cancer. extract were investigated on human cancer cells (PA-TU-8902) and immortalized endothelial-like (Ea.hy926). PA-TU-8902 tumours xenografted athymic mice also examined. In vitro migration invasiveness assays...
Background/Aim: Pancreatic ductal adenocarcinoma (PDAC) still represents one of the most aggressive cancers. Understanding epithelial–mesenchymal crosstalk as a crucial part tumor microenvironment should pave way for therapies to improve patient survival rates. Well-established cell lines present useful and reproducible model study PDAC biology. However, tumor–stromal interactions between cancer cells cancer–associated fibroblasts (CAFs) are poorly understood. Materials Methods: We studied...
Aberrant regulation of the cell cycle is a typical feature all forms cancer. In head and neck squamous carcinoma (HNSCC), it often associated with overexpression cyclin D1 (CCND1). However, remains unclear how CCND1 expression changes between tumor normal tissues whether human papillomavirus (HPV) affects differential expression. Here, we evaluated D-type cyclins in cohort 94 HNSCC patients which 82 were subjected to whole genome profiling primary tumors paired mucosa. Comparative analysis...
DNA origami nanoframes with two parallel sequences are used to evaluate the effect of nucleoside substituents on radiation-induced damage. Double strand breaks (DSB) counted using atomic force microscopy (AFM), and total number lesions is evaluated real-time polymerase chain reaction (RT-PCR). Enhanced AT or GC content does not increase breaks. Incorporation 8-bromoadenosine results in highest enhancement lesions; however, DSB observed for 2′-deoxy-2′-fluorocytidine, indicating different...
Abstract Statins, the drugs used for treatment of hypercholesterolemia, have come into spotlight not only as chemoadjuvants, but also potential stem cell modulators in context regenerative therapy. In our study, we compared vitro effects all clinically statins on viability human pancreatic cancer (MiaPaCa-2) cells, non-cancerous embryonic kidney (HEK 293) cells and adipose-derived mesenchymal (ADMSC). Additionally, effect MiaPaCa-2 ADMSC spheroids was tested. Furthermore, performed a...
Having previously initiated genome-wide expression profiling in head and neck squamous cell carcinoma (HNSCC) for regions of the tumor, margin surgical resecate (MSR) normal mucosa (NM), we here proceed with respective analysis cases after stratification according to status tenascin (Ten).Tissue specimens each anatomical site were analyzed by immunofluorescent detection Ten, fibronectin (Fn) galectin-1 (Gal-1) as well microarrays.Histopathological examination demonstrated that Ten+Fn+Gal-1+...
With the increasing demand for noninvasive approaches in monitoring head and neck cancer, circulating nucleic acids have been shown to be a promising tool. We focused on global transcriptome of serum samples squamous cell carcinoma (HNSCC) patients comparison with healthy individuals. compared gene expression patterns 36 samples. Twenty-four participants including 16 HNSCC (from 12 we obtained blood 1 year posttreatment) 8 control subjects were recruited. The Illumina HumanWG-6 v3 Expression...
Breast cancer is the most frequently diagnosed in women worldwide. Although dramatically increased survival rates of early cases have been observed, late patients and metastatic may still be considered fatal. The present study's main focus was on cancer‑associated fibroblasts (CAFs) which an active component tumor microenvironment (TME) regulating breast ecosystem. Transcriptomic profiling analysis CAFs isolated from skin metastasis, cutaneous basal cell carcinoma, squamous carcinoma...
Abstract BACKGROUND Brain metastases (BrM) are a frequent complication of lung cancer with dismal prognosis. The presence associated fibroblasts (CAF) was recently described in the BrM microenvironment, but their engagement biology is largely unknown. In this study, we evaluated effect CAF derived from brain (BrM-CAF) on cells and recruitment differentiation monocytes. MATERIAL AND METHODS Patient-derived BrM-CAF (BrM-CC) were isolated BrM. Normal subgalear connective tissue. Monocytes...
Altering amounts of a protein in cell has become crucial tool for understanding its function. In many organisms, including the protozoan parasite Trypanosoma brucei, overexpression been achieved by inserting protein-coding sequence into an vector. Here, we have adapted PCR only based system tagging trypanosome proteins at their endogenous loci such that it addition enables tetracycline-inducible T7 RNA polymerase-mediated overexpression. Hence, this approach bypasses need molecular cloning,...