A5030234822- Pain Mechanisms and Treatments
- Ion Channels and Receptors
- Neuropeptides and Animal Physiology
- Pancreatic and Hepatic Oncology Research
- Congenital Anomalies and Fetal Surgery
- MicroRNA in disease regulation
- Cancer-related molecular mechanisms research
- Dental Implant Techniques and Outcomes
- Cancer Research and Treatments
- Cancer Cells and Metastasis
- Asthma and respiratory diseases
- Cancer Genomics and Diagnostics
- Renal and related cancers
- Forensic Anthropology and Bioarchaeology Studies
- Systemic Sclerosis and Related Diseases
- Circular RNAs in diseases
- Dental Radiography and Imaging
- Cardiovascular Issues in Pregnancy
- 3D Printing in Biomedical Research
- Aortic aneurysm repair treatments
- Respiratory and Cough-Related Research
- Assisted Reproductive Technology and Twin Pregnancy
- Aortic Disease and Treatment Approaches
- Urological Disorders and Treatments
- Orthopaedic implants and arthroplasty
Charles University
2015-2024
University Hospital Kralovske Vinohrady
2015-2024
University of Szeged
2001-2024
University of Toronto
2024
Hospital for Sick Children
2024
SickKids Foundation
2024
Great Ormond Street Hospital
2011
University College London
2011
University College London Hospitals NHS Foundation Trust
2011
University of Pecs
2004-2009
Capsaicin-sensitive, TRPV1 (transient receptor potential vanilloid 1) receptor-expressing primary sensory neurons exert local and systemic efferent effects besides the classical afferent function. The is considered a molecular integrator of various physico-chemical noxious stimuli. In present study its role was analysed in acute nociceptive tests chronic neuropathy models by comparison wild-type (WT) knockout (KO) mice. formalin-induced nocifensive behaviour, carrageenan-evoked inflammatory...
We previously demonstrated that somatostatin (SOM) released from the activated peripheral terminals of capsaicin-sensitive primary sensory neurons inhibits acute inflammation and nociception. This study was undertaken to examine this systemic "sensocrine" function neuronally derived in chronic Freund's complete adjuvant (CFA)-induced arthritis model.Arthritis tibiotarsal joint Lewis rats evoked by subcutaneous injection CFA into left hind paw tail root. For 3 weeks, volume paws measured...
The transient receptor potential vanilloid 1 (TRPV1) is a nonselective cation channel localized on subset of primary sensory neurons and can be activated by wide range stimuli. present study investigated the role this in chronic arthritis evoked complete Freund9s adjuvant (CFA) using TRPV1 gene-deleted (TRPV1<sup>-/-</sup>) mice wild-type counterparts (TRPV1<sup>+/+</sup>). In TRPV1<sup>+/+</sup> mice, CFA injected intraplantarly into left hindpaw root tail induced swelling contralateral...
Airways are densely innervated by capsaicin-sensitive sensory neurons expressing transient receptor potential vanilloid 1 (TRPV1) receptors/ion channels, which play an important regulatory role in inflammatory processes via the release of neuropeptides. The aim present study was to investigate TRPV1 receptors endotoxin-induced airway inflammation and consequent bronchial hyperreactivity with functional, morphological, biochemical techniques using gene-deficient mice. Inflammation evoked...
To determine whether sonographic findings in cases of exomphalos detected at the 11-14-week scan can be used to guide pregnancy management.Retrospective study identified from Fetal Medicine Unit database, University College London Hospitals between January 1998 and 2010. Pregnancy neonatal data were ascertained maternal records. was categorized into three groups: associated with other major structural malformation(s), isolated increased nuchal translucency (NT) normal NT.A total 98...
Dental implants are artificial dental roots anchoring prosthetic restorations to replace natural teeth. implant systems may have different tapered conical connections. Our research focused on the mechanical examination of implant–superstructure Thirty-five samples with 5 cone angles (24°, 35°, 55°, 75°, and 90°) were tested for static dynamic loads, carried out by a fatigue testing machine. Fixing screws fixed torque 35 Ncm before measurements. For loading, loaded force 500 N in 20 s. 15,000...
Background and Purpose — Atherosclerotic middle cerebral arteries are frequent sites of thrombosis, leading to stroke. Previous studies have suggested a role for Chlamydia pneumoniae in the pathogenesis atherosclerosis. However, presence this pathogen atherosclerotic has heretofore not been documented. In present study, we analyzed atheromatous plaques from C . Methods 15 cadavers who died natural causes corresponding nonatherosclerotic 4 otherwise healthy trauma victims were examined....
RT-qPCR quantification of miRNAs expression may play an essential role in pancreatic ductal adenocarcinoma (PDAC) diagnostics. RT-qPCR-based experiments require endogenous controls for the result normalization and reliability. However, instability reference genes tumors introduce bias when determining miRNA levels. We investigated 6 miRNAs, isolated from FFPE samples adenocarcinomas. Four internal were utilized normalization: artificial miR-39 C. elegans, U6 snRNA, miR-16 snoRNA U91. found...
Germ cell tumors and particularly seminomas reflect the epigenomic features of their parental primordial germ cells (PGCs), including genomic DNA hypomethylation expression pluripotent markers. Because might be a result TET dioxygenase activity, we examined TET1-3 enzymes level product, 5-hydroxymethylcytosine (5hmC), in panel histologically characterized non-seminomatous tumors. Expression mRNAs was quantified by real-time PCR. TET1 5hmC were immunohistochemically. Quantitative assessment...
Background/Aim: Pancreatic ductal adenocarcinoma (PDAC) still represents one of the most aggressive cancers. Understanding epithelial–mesenchymal crosstalk as a crucial part tumor microenvironment should pave way for therapies to improve patient survival rates. Well-established cell lines present useful and reproducible model study PDAC biology. However, tumor–stromal interactions between cancer cells cancer–associated fibroblasts (CAFs) are poorly understood. Materials Methods: We studied...
Abstract Objective Along with their classic afferent function (nociception), capsaicin‐sensitive transient receptor potential vanilloid 1 (TRPV1) receptor–expressing sensory nerve terminals exert local and systemic efferent activities. Activation of TRPV1 causes neuropeptide release, which modulates the inflammation process. The aim present study was to examine role this modulatory that calcitonin gene‐related peptide (CGRP) in bleomycin‐induced scleroderma, using transgenic mice. Methods...
Somatostatin released from capsaicin-sensitive sensory nerves of the lung during endotoxin-induced murine pneumonitis inhibits inflammation and hyperresponsiveness, presumably via somatostatin receptor subtype 4 (sst ). The goal present study was to identify sst receptors in mouse human lungs reveal its inflammation-induced alterations with real-time quantitative PCR, Western blot, immunohistochemistry. In non-inflamed lungs, mRNA expression immunolocalization are very similar. They on...
Pancreatic cancer is a severe malignancy with increasing incidence and high mortality due to late diagnosis low sensitivity treatments. Search for the most appropriate drugs therapeutic regimens promising way improve treatment outcomes of patients. This study aimed compare (1)
ABSTRACT Objective Fetuses with single ventricle physiology (SVP) exhibit reductions in fetal cerebral oxygenation, associated delays brain growth and neurodevelopmental outcomes. Maternal supplemental oxygen (MSO) has been proposed to improve growth, but current evidence on dosing, candidacy outcomes is limited. In this pilot study, we evaluated the safety feasibility of continuous low‐dose MSO setting SVP. Methods This single‐center, open‐label, phase‐1 clinical trial included 25 pregnant...
Undifferentiated carcinoma with osteoclast-like giant cells (UCOGC) of the pancreas represents a rare subtype pancreatic ductal adenocarcinoma (PDAC). Despite distinct morphology and specific clinical behavior, UCOGCs exhibit unexpected similarities in regard to DNA mutational profiles conventional PDAC. Treating is particularly challenging, limited prospects for cure. As many other malignant neoplasms, exploration microRNAs (miRNAs, miRs) regulating biological characteristics cancer...
Repeated administration of amphetamine derivatives is reported to induce neurotoxicity in rat brain. Methamphetamine (MA) impairs the function both dopaminergic and serotonergic systems, 3,4-methylenedioxymethamphetamine (MDMA, Ecstasy) affects primarily latter system. The neurochemical deficits induced by these amphetamines have been described detail, but relatively few data regarding behavioural consequences neurotoxic treatment. aim present study was investigate short- long-term treatment...
Pancreatic ductal adenocarcinoma (PDAC) remains a disease with extremely poor prognosis and limited effective available treatment.Differential expression of miRNAs isolated from tumor tissue has been proposed as marker for diagnosis, progression, prognosis.Nevertheless, the prognostic value in PDACs patient outcome still unclear.Expression 7 selected miRNAs, FFPE samples 54 PDAC patients, was quantified using RT-qPCR.The relationship miRNA levels histology, clinicopathological...