A5057142178- Organ Transplantation Techniques and Outcomes
- Liver Disease Diagnosis and Treatment
- Liver Disease and Transplantation
- Liver physiology and pathology
- Hippo pathway signaling and YAP/TAZ
- Endoplasmic Reticulum Stress and Disease
- Autophagy in Disease and Therapy
- Cholangiocarcinoma and Gallbladder Cancer Studies
- Circular RNAs in diseases
- Phagocytosis and Immune Regulation
- Hepatocellular Carcinoma Treatment and Prognosis
- Genomics, phytochemicals, and oxidative stress
- Advanced Glycation End Products research
- Macrophage Migration Inhibitory Factor
- Inflammasome and immune disorders
- Cancer-related molecular mechanisms research
- MicroRNA in disease regulation
- Mitochondrial Function and Pathology
- Drug Transport and Resistance Mechanisms
- Renal Transplantation Outcomes and Treatments
- Sphingolipid Metabolism and Signaling
- Ubiquitin and proteasome pathways
- Immune Cell Function and Interaction
- Cancer Mechanisms and Therapy
- T-cell and B-cell Immunology
Nanjing Medical University
2016-2025
Chinese Academy of Medical Sciences & Peking Union Medical College
2018-2025
Jiangsu Province Hospital
2016-2025
People’s Hospital of Linqing
2024
Institute of High Energy Physics
2024
Chinese Academy of Sciences
2024
National Health and Family Planning Commission
2018-2023
Nanjing Normal University
2022
Jiangsu Cancer Hospital
2020-2021
State Key Laboratory of Reproductive Medicine
2021
Follistatin-like protein 1 (FSTL1) is widely recognised as a secreted glycoprotein, but its role in modulating macrophage-related inflammation during liver fibrosis has not been documented. Herein, we aimed to characterise the roles of macrophage FSTL1 development fibrosis.Expression analysis was conducted with human samples obtained from 33 patients and 18 individuals without serving controls. Myeloid-specific FSTL1-knockout (FSTL1M-KO) mice were constructed explore function mechanism 3...
Although the roles of metabolic stress in organ ischemia-reperfusion injury (IRI) have been well recognized, question whether and how these responses regulate innate immune activation against IR remains unclear. In a murine liver partial warm ischemia mode, we showed that prolonged triggered endoplasmic reticulum (ER) response, particularly, activating transcription factor 6 (ATF6) branch, Kupffer cells (KCs) altered their responsiveness Toll-like receptor (TLR) stimulation. Ischemia-primed...
Although aggravated liver injury has been reported in aged livers post-ischemia and reperfusion (IR), the underlying mechanism of innate immune activation macrophages is not well understood. Here, we investigated whether how Stimulator interferon genes (STING) signaling regulated macrophage proinflammatory IR injury. Mice were subjected to hepatic vivo. Macrophages isolated from IR-stressed bone marrow-derived (BMDMs) young mice used for vitro studies. Enhanced nucleotide-binding domain...
Abstract Growing evidence suggests that aberrant microRNA (miRNA) expression contributes to hepatocellular carcinoma (HCC) development and progression. However, the potential role mechanism of miR-665 in progression liver cancer remains largely unknown. Our current study showed was upregulated HCC cells tissues. High exhibited more severe tumor size, vascular invasion Edmondson grading patients. Gain- or loss-of-function assays demonstrated promoted cell proliferation, migration, invasion,...
Abstract Although the role of phosphatase and tensin homolog deleted on chromosome 10 (PTEN) in regulating cell proliferation is well established, its function immune responses remains to be fully appreciated. In current study, we analyzed myeloid-specific PTEN tissue inflammatory response a murine liver partial warm ischemia model. Myeloid-specific knockout (KO) resulted protection from reperfusion injury (IRI) by deviating local innate against toward regulatory type: expression...
Metastasis is the major cause of poor prognosis hepatocellular carcinoma (HCC), and increasing evidence supports contribution miRNAs to cancer progression. However, exact relationship between level miR-1301 expression HCC cell migration, invasion, angiogenesis remains largely unknown. Quantitative PCR was used evaluate in tissues lines. Transwell tube-formation assays were measure effects on migration angiogenesis, respectively. Luciferase reporter western blotting confirm target genes. We...
CCAAT/enhancer binding protein (C/EBP)-homologous (CHOP) has been shown to be a key molecule in endoplasmic reticulum (ER) stress-mediated apoptosis. ER oxidoreductin 1-α (ERO1α), target of CHOP, is an important oxidizing enzyme that regulates reactive oxygen species (ROS), which play prominent role hepatocellular death during acute liver failure (ALF). However, little known about how CHOP facilitates ROS-induced injury. The present study was designed investigate the roles and molecular...
Abstract Background and Aims Accumulating studies identified that BUB1 mitotic checkpoint serine/threonine kinase B (BUB1B) is integrally involved in the initiation development of tumors. Nevertheless, precise biological role underlying mechanisms BUB1B hepatocellular carcinoma (HCC) remain indistinct. Method To figure out HCC, we first assessed its expression using The Cancer Genome Atlas (TCGA) Gene Expression Profiling Interactive Analysis (GEPIA) databases. We then verified HCC tissues,...
Immune checkpoint inhibitors plus antiangiogenic tyrosine kinase may offer a first-line treatment for advanced hepatocellular carcinoma (HCC). In this phase 2 trial [registered with clinicaltrials.gov (NCT04052152)], we investigated the safety and efficacy of anti-PD-1 antibody sintilimab TKI anlotinib HCC.Pathologically-proven HCC patients received (200 mg) on day 1 (12 once daily days to 14 every 3 weeks, run-in first six participants assess dose-limiting toxicities (DLTs). The primary...
Abstract Mounting evidence demonstrates that expression of ERO1α, an endoplasmic reticulum (ER)-resident oxidase, is a poor prognosis factor in variety human cancers. However, the clinical relevance ERO1α and its molecular mechanisms underlying tumor progression have not been determined for hepatocellular carcinoma (HCC). levels HCC tissues cells were detected by quantitative real-time PCR western blotting. shRNAs overexpression vector transfected into to downregulate or upregulate...
Abstract Background and Aims Nuclear factor erythroid 2‐related 2 (Nrf2) is a master regulator of reactive oxygen species (ROS) inflammation has been implicated in both human murine inflammatory disease models. We aimed to characterize the roles macrophage‐specific Nrf2 liver ischemia/reperfusion injury (IRI). Approach Results First, macrophage expression patients undergoing OLT or ischemia‐related hepatectomy were analyzed. Subsequently, we created myeloid‐specific Nrf2‐knockout (Nrf2 M‐KO...
ABSTRACT The global incidence of biliary tract cancer (BTC) is on the rise, presenting a substantial healthcare challenge. integration immune checkpoint inhibitors (ICIs) with molecularly targeted therapies emerging as strategy to enhance responses. However, efficacy and underlying mechanisms these treatments in BTC are still largely unexplored. In this study, tissue samples from 19 patients treated camrelizumab apatinib were analysed using NanoString 289‐panel identify key molecular...
Liver transplantation (LT) remains the primary treatment for irreversible liver failure. However, challenges in organ preservation, particularly ischemia-reperfusion injury and donor vulnerability, contribute to poor post-transplant outcomes.[1,2] The accumulation of reactive oxygen species (ROS) exacerbates these issues, leading inflammation, tissue damage, impaired graft function.[3] Ceria nanoparticles have gained attention their antioxidant, antibacterial, antifibrotic properties...
All-trans retinoic acid (atRA) is an active metabolite of vitamin A with antioxidant effects. There have been few reports on the effects atRA liver ischemia/reperfusion (I/R) injury. Here we used a rat ischemia/ reperfusion model to analyze protective effect atRA. Rats were administered different does (5—15 mg/kg/d) intraperitoneally (i.p.) for 14 d before I/R. Partial (70%) hepatic ischemia was induced by clamping artery, portal vein, and bile duct left median lobes using vascular clamp 60...
Background Low-dose lipopolysaccharide (LPS) preconditioning-induced liver protection has been demonstrated during ischemia-reperfusion injury (IRI) in several organs but not sufficiently elucidated underlying causal mechanism. This study investigated the role of low-dose LPS preconditioning on ATF4-CHOP pathway as well effects tissue and inflammation a mouse model partial-warm IRI. Methods (100 µg/kg/d) was injected intraperitoneally two days before ischemia. Hepatic evaluated based serum...
Background/Aims: Immunosuppression is one of the hallmarks cancer; however, its molecular mechanism remains unknown. In present study, we sought to investigate expression and activation yes-associated protein 1 (YAP-1) roles in T cells within hepatocellular carcinoma (HCC). Methods: The YAP-1 were accessed by real-time PCR, immunohistochemistry staining, western blot, flow cytometry. potential regulation effect on Regulatory (Tregs) differentiation was predicted using bioinformatics tools...
Lycopene is a naturally occurring carotenoid found in many fruits and vegetables, which has antioxidant effects. Although lycopene's protective effect been observed on ischemia reperfusion (IR) injury different organs, the of lycopene Kupffer cells (KCs) not clearly elucidated IR-induced acute hepatic inflammatory injury.Mice were administered with either olive oil (10 mL/kg body weight) as control or (20 mg/kg by gavage for 2 weeks before undergoing IR injury.In this study, we that levels...
Nuclear-erythroid-2-related factor 2 (Nrf2) is involved in the pathogenesis of different liver diseases. Herein, we first demonstrated that Nrf2 expression was diminished nonalcoholic steatohepatitis (NASH) macrophages. In myeloid Nrf2-deficiency mice, aggravated steatosis and inflammation high-fat-diet (HFD)-fed mice were observed compared with chow-diet group. Moreover, increasing inflammatory cytokines influenced lipid metabolism hepatocytes vivo vitro. Further study showed regulated...
Abstract Liver fibrosis is a progressive disease accompanied by the deposition of extracellular matrix (ECM). Numerous reports have demonstrated that alterations in expression microRNAs (miRNAs) are related to liver disease. However, effect individual miRNAs on has not been studied. Hepatic stellate cells (HSCs), being responsible for producing ECM, exert an important influence fibrosis. Then, microarray analysis non‐activated and activated HSCs induced transforming growth factor β1 (TGF‐β1)...