A5032338743- Cancer Cells and Metastasis
- 3D Printing in Biomedical Research
- Nanoparticle-Based Drug Delivery
- Reproductive System and Pregnancy
- Endometriosis Research and Treatment
- Uterine Myomas and Treatments
- RNA Interference and Gene Delivery
- Tissue Engineering and Regenerative Medicine
- Coagulation, Bradykinin, Polyphosphates, and Angioedema
- Signaling Pathways in Disease
- Advanced Drug Delivery Systems
- Estrogen and related hormone effects
- Parvovirus B19 Infection Studies
- Neonatal Respiratory Health Research
- Histiocytic Disorders and Treatments
- Graphene and Nanomaterials Applications
- Viral-associated cancers and disorders
- Cancer Research and Treatment
- Advanced Breast Cancer Therapies
- Vitamin C and Antioxidants Research
- Advancements in Transdermal Drug Delivery
- Pancreatic function and diabetes
- Inhalation and Respiratory Drug Delivery
- Chemical Reactions and Isotopes
- Pain Management and Placebo Effect
Sahlgrenska University Hospital
2021-2023
University of Gothenburg
2020-2023
Hospital Metropolitano
2021
Universidad de Granada
2015-2019
Instituto de Investigación Biosanitaria de Granada
2015-2019
Andalusian Health Service
2015
Oregon Clinic
2014
Providence College
2014
Oregon Health & Science University
2014
Southwest Washington Medical Center
2014
Maternal smoking during pregnancy adversely affects offspring lung development, with lifelong decreases in pulmonary function and increased asthma risk. In a primate model, vitamin C blocked some of the in-utero effects nicotine on development function.To determine if newborns pregnant smokers randomized to receive daily would have improved results tests (PFTs) decreased wheezing compared those placebo.Randomized, double-blind trial conducted 3 sites Pacific Northwest between March 2007...
During the purification of eosinophil chemotactic factors synthesized by uterus in response to estrogen we isolated a protein having an N-terminal (15 amino acids) sequence identical that rat cyclophilin. Our data demonstrate cyclophilin, cytosolic from bovine thymocytes, which specifically binds immunosuppressive drug cyclosporin A, as well recombinant human displays activity. In addition its activity, cyclophilin stimulated release peroxidase activity eosinophils. Maximal was achieved at...
Abstract Three-dimensional cell culture platforms based on decellularised patient-based microenvironments provide in vivo-like growth conditions allowing cancer cells to interact with intact structures and components of the surrounding tissue. A patient-derived scaffold (PDS) model was therefore evaluated as a testing platform for endocrine therapies (Z)-4-Hydroxytamoxifen (4OHT) fulvestrant well CDK4/6-inhibitor palbociclib, monitoring treatment responses breast lines MCF7 T47D adapted...
To describe fetal cardiac and hemodynamic development in the first trimester of pregnancy.Forty-eight pregnancies were prospectively studied with transvaginal ultrasound color Doppler. Fetal heart rate size documented together presence inflow outflow wave-forms valve signals.Heart showed a positive correlation gestational age. At 6 weeks gestation, 20% cycle was occupied by isovolumic contraction time (ICT); relaxation (IRT) 16% remained unchanged, while ICT progressively shortened not...
Paclitaxel (PTX) is one of the drugs choice in treatment breast and lung cancer. However, its severe side effects, including mielosuppression, cardiotoxicity neurotoxicity, frequently cause to be discontinued. Solid lipid nanoparticles (NPs) glyceril tripalmitate (tripalmitin) loaded with PTX (Tripalm-NPs-PTX) modifications by addition hexa(ethylene glycol), β-cyclodextrin macelignan were developed. All NPs-PTX formulations displayed excellent hemocompatibility significantly enhanced...
Abstract Breast cancer is a heterogeneous disease where the tumor microenvironment, including extracellular components, plays crucial role in progression, potentially modulating treatment response. Different approaches have been used to develop three‐dimensional models able recapitulate complexity of matrix. Here, we use cell‐free patient‐derived scaffolds (PDSs) generated from breast samples that were recellularized with cell lines as an vivo‐like culture system for drug testing. We show...
Abstract The cancer microenvironment influences tumor progression and metastasis is pivotal to consider when designing in vivo -like models. Current preclinical testing platforms for drug development are mainly limited 2D cell culture systems that poorly mimic physiological environments traditional, low throughput animal aim of this work was produce a tunable platform based on 3D printed scaffolds (3DPS) with simple geometry that, by extracellular components response breast reporter cells,...
Breast cancer is a heterogeneous disease in terms of cellular and structural composition, besides acquired aggressive properties the cell population, surrounding tumor microenvironment can affect progression clinical behaviours. To specifically decode relevance promoting effects individual microenvironments, we performed comprehensive test 110 breast samples using recently established vivo-like 3D culture platform based on patient-derived scaffolds (PDSs). Cell-free PDSs were recellularized...
Cationically modified solid lipid nanoparticles (SLN) were investigated as plasmid DNA (pDNA) carriers and transfection agents for the pulmonary route.pDNA-loaded SLN produced using glyceryl dibehenate or tristearate matrix lipids chitosan surface charge modifier, encapsulated by spray-drying in mannitol trehalose microspheres.Nanoparticles of 200 nm, zeta potential around +15 mV produced. Electrophorectic analysis confirmed stability integrity. The pDNA-loaded able to transfect Calu-3 A549...
Cyclophilin, the cyclosporin A binding protein and member of immunophilin family proteins, demonstrates leukocyte chemotactic activity. In this study we demonstrate that FKBP, FK506 rapamycin protein, also displays The activity FKBP is inhibited by FK506, however, was unable to inhibit cyclophilin-stimulated Rapamycin prevent similarly, CsA analogue Me6Ala-CsA while displaying cyclophilin block These results suggest in addition their intracellular role immunophilins may function as agents,...
Cancer is a disease in which cells show an atypical behavior and begin to divide abnormally without control. Furthermore, these can spread other tissues result the development of new tumors. It very difficult find treatment that discriminates between tumor healthy because cancerous are from person who has they not external foreign pathogen easily recognizable by immune system. Thus, treatments currently exist for cancer quite nonspecific also attack cells, producing highly specific toxicity...
The ontogeny of an estrogen-induced eosinophil chemotactic activity in the uterus after estrogen administration and its regulation by progesterone was studied neonatal rats at 3, 7, 10, 13 days age. These results were compared with increase peroxidase secretion complement component C3 uterus. No observed animals younger than 14 days, this age response also prevented co-administration progesterone. appeared earlier, 10 It that 3–7-day-old responded to increasing two proteins 36 110 kDa....
Abstract Background The tumor microenvironment clearly influences cancer progressing properties but less is known about how individual microenvironments potentially moderate treatment effects. By cultivating and treating cell lines in patient-derived scaffolds (PDS), the impact of specific characteristics can be incorporated human-like growth modelling drug testing. Methods PDSs from 78 biobanked primary breast samples with patient outcomes, were prepared repopulated donor lines, followed by...
Offspring of smokers who continue to smoke during pregnancy show adverse effects in their lung development, with lifelong decreases pulmonary function. Clinical trials smoking cessation have been ineffective. This randomized, double-blind, placebo-controlled study was performed determine whether vitamin C supplementation the maternal on newborn function test (PFT) results. Pregnant received either (500 mg/d) or placebo; pregnant nonsmokers constituted a reference group. Parturients were 15...