A5039670869- Cutaneous lymphoproliferative disorders research
- Lymphoma Diagnosis and Treatment
- Synthesis and Biological Evaluation
- Chronic Lymphocytic Leukemia Research
- Click Chemistry and Applications
- Synthesis and biological activity
- Chemical Synthesis and Analysis
- Bioactive Compounds and Antitumor Agents
- Neutrophil, Myeloperoxidase and Oxidative Mechanisms
- T-cell and Retrovirus Studies
- Cell Adhesion Molecules Research
- Quinazolinone synthesis and applications
- Telomeres, Telomerase, and Senescence
- Synthesis and Characterization of Heterocyclic Compounds
- interferon and immune responses
- Influenza Virus Research Studies
- Leptospirosis research and findings
- Atherosclerosis and Cardiovascular Diseases
- Phenothiazines and Benzothiazines Synthesis and Activities
- Ginger and Zingiberaceae research
- Cytokine Signaling Pathways and Interactions
- Genomics, phytochemicals, and oxidative stress
- Nanoplatforms for cancer theranostics
- Marine Sponges and Natural Products
- CAR-T cell therapy research
The University of Texas Medical Branch at Galveston
2024
The University of Texas MD Anderson Cancer Center
2023-2024
Houston Methodist
2021-2022
Indian Institute of Chemical Technology
2011-2019
University of Hyderabad
2019
Spatane diterpinoids isolated from the brown marine algae Stoechospermum marginatum were known to have cytotoxic effects in human cancerous cell lines and murine melanoma cells; underling apoptotic mechanism of still remains unclear so far. Thus, present study, a spatane diterpinoid, 5(R), 19‐diacetoxy‐15,18(R S), dihydro spata‐13, 16(E)‐diene (DDSD) was investigated mainly B16F10 cells because they most susceptible DDSD than THP1, U937, COLO205, HL60 cells. The treatment B6F10 with resulted...
A series of 3-[3-[4-(Substituted)-1-cyclicamine]propyl]thio-5-substituted[1,2,4]triazoles (8a-j) were synthesized with good yields starting from corresponding carboxylic acids. The cytotoxicity studies these derivatives studied against five different human cancer cell lines. Three compounds had shown anticancer activity. triazole derivatives, 8i and 8j most potent particularly U937 HL-60 cells. cytotoxic potency the varied between lines suggesting that a structural property as possible...
Proper cell division relies on the coordinated regulation between a structural component, mitotic spindle, and regulatory anaphase-promoting complex/cyclosome (APC/C). Hematopoietic PBX-interacting protein (HPIP) is microtubule-associated that plays pivotal role in proliferation, migration, tumor metastasis. Here, using HEK293T HeLa cells, along with immunoprecipitation immunoblotting, live-cell imaging, protein-stability assays, we report HPIP expression oscillates throughout cycle its...
Constitutively activated tyrosine kinase JAK3 is implicated in the pathogenesis of cutaneous T-cell lymphomas (CTCL). The mechanisms constitutive activation are unknown although a mutation was reported small portion CTCL patients. In this study, we assessed oncogenic roles newly identified JAK3-INSL3 fusion transcript CTCL. Total RNA from malignant T-cells 33 patients with Sézary syndrome (SS), leukemic form CTCL, examined for new by RT-PCR followed Sanger sequencing. expression levels were...
The 2-amino-6-trifluoromethyl-3H-pyrimidin-4-one 1 was propargylated to give two regioisomers 2, 3 in definite proportions. Both 2 and were independently reacted with alkyl, aryl or cycloalkyl substituted azides under Sharpless conditions obtained exclusively 1,4–disubstituted triazole tagged trifluoromethyl pyrimidine derivatives 4 5 respectively. All the final products evaluated for cytotoxic activity against four cancer cell lines promising compounds identified. Keywords: Alkylation,...
We have shown that membrane-associated guanylate kinase with inverted domain structure-1 (MAGI1), a scaffold protein six PSD95/DiscLarge/ZO-1 (PDZ) domains, is involved in the regulation of endothelial cell (EC) activation and atherogenesis mice. In addition to causing acute respiratory disease, influenza A virus (IAV) infection plays an important role triggers coronary syndromes fatal myocardial infarction. Therefore, aim this study investigate function MAGI1 IAV-induced EC activation....
Abstract Two series of diaziridinyl quinone isoxazole derivatives were prepared and evaluated for their cytotoxic activity against MCF7, HeLa, BT549, A549 HEK293 cell lines interaction with tubulin. Compounds (6a–m ) showed promising all the 5 human cancer lines. 6a, 6e 6 m potent [IC50 ranging between 2.21 µg to 2.87 µg] on ER-positive MCF7 line similar commercially available drug molecule Doxorubicin. The results from docking models are in consistent experimental values which demonstrated...
Abstract Mycosis fungoides (MF) and Sézary syndrome (SS) are two most common types of cutaneous T-cell lymphoma (CTCL). Despite advances in understanding the pathogenesis MF SS, effective treatments remain limited. CC chemokine receptor 4 (CCR4) is highly expressed on CTCL cells serves as a great therapeutic target. Mogamulizumab, an FDA-approved anti-CCR4 antibody, has shown efficacy treating MF/SS; however, its side effects have raised concerns, underscoring need for more less toxic...
<div>Abstract<p>Mycosis fungoides (MF) and Sézary syndrome (SS) are two most common types of cutaneous T-cell lymphoma (CTCL). Despite advances in understanding the pathogenesis MF SS, effective treatments remain limited. CC chemokine receptor-4 (CCR4) is highly expressed on CTCL cells serves as a great therapeutic target. Mogamulizumab, an FDA-approved anti-CCR4 antibody, has shown efficacy treating MF/SS; however, its side effects have raised concerns, underscoring need for...
<p>Chemotaxis of MJ and HuT 78 cells to CCL22 CCL17 inhibitory effects CCR4 antagonists on chemotaxis. <b>A,</b> Transwell plates were used for the chemotaxis assay. Chemotaxis series doses or [log(M)] was assessed after 3 hours, data are plotted. <b>B,</b> For assay, first treated with C021 AZD-2098 30 minutes then The dose-dependent inhibition (100 nmol/L) (10 is plotted cells, respectively.</p>
<p>Two allosteric CCR4 antagonists and their effects on expression in MJ HuT 78 cells. <b>A,</b> Schematic cartoon of binding sites two antagonists: C021 binds to a transmembrane site (site 1, red), AZD-2098 intracellular or C-terminal 2, orange); <b>B,</b> cells treated with (green lines) DMSO (red was assessed by flow cytometry anti-CCR4 antibody (CCR4-PE-CF594 antibody, IgG1, κ, Clone: 1G1); <b>C,</b> The mRNA (gray) (black) qRT-PCR. fold changes...
<p>IC<sub>50</sub> of CCR4 antagonists on chemotaxes and cell proliferation in MJ HuT 78 cells</p>
<p>Effects of C021 on tumor formation and growth in CTCL xenograft mice. <b>A,</b> Schedules treatment HuT 78 cells (5.0 × 10<sup>6</sup>/site, nine mice/group) were subcutaneously injected into NSG mice day 1. The started 2, four groups treated with different doses, routes, schedules as indicated: <b>G1</b>—control (Ctl), i.p., twice/week; <b>G2</b>—5 mg/kg, <b>G3</b>—2 daily thrice/week; <b>G4</b>—5 s.c.,...
<p>Effects of C021 and AZD-2098 on apoptosis cell cycle in MJ HuT 78 cells. <b>A,</b> cells (5 × 10<sup>5</sup>/mL) were treated with different doses or vehicle control for 12 hours. Apoptotic assessed by flow cytometry using the annexin V–FITC Detection Kit. Dot plots from analysis are presented. <b>B,</b> at 25.0 μmol/L hours, dot <b>C,</b> (proliferation IC<sub>50</sub>, 10.0, μmol/L) Cell-cycle was performed assessing DNA...
<p>Effects of C021 on cell proliferation and colony formation in MJ HuT 78 cells. <b>A</b> <b>B,</b> cells were seeded 96-well plates treated with series doses or vehicle controls for 12 hours. The viability was then determined using the CellTiter 96 AQueous One Solution Cell Proliferation Assay (MTS). data are analyzed plotted. IC<sub>50</sub> calculated <b>C</b> <b>D,</b> Soft agar assay used to assess effects...