A5102814757- DNA Repair Mechanisms
- Genomics and Chromatin Dynamics
- Epigenetics and DNA Methylation
- RNA Research and Splicing
- History, Culture, and Diplomacy
- RNA and protein synthesis mechanisms
- RNA modifications and cancer
- Sperm and Testicular Function
- Genetic Neurodegenerative Diseases
- Molecular Biology Techniques and Applications
- Immune Cell Function and Interaction
- Historical and Literary Studies
- DNA and Nucleic Acid Chemistry
- Mitochondrial Function and Pathology
- COVID-19 Impact on Reproduction
- Metabolism, Diabetes, and Cancer
- Regulation of Appetite and Obesity
- Classical Studies and Philology
- Chromosomal and Genetic Variations
- Genetics and Neurodevelopmental Disorders
- Historical and Literary Analyses
- Animal Genetics and Reproduction
- Historical Studies in Science
- IL-33, ST2, and ILC Pathways
- Angiogenesis and VEGF in Cancer
University of Naples Federico II
2012-2024
École des hautes études en sciences sociales
2018-2023
Centre Alexandre Koyré
2018-2023
Federico II University Hospital
2018-2020
University of Padua
2019
Centre National de la Recherche Scientifique
2018
Campus Condorcet
2018
Istituto Nazionale di Fisica Nucleare, Sezione di Napoli
2015
Sheba Medical Center
1988
Abstract We characterize the changes in chromatin structure, DNA methylation and transcription during after homologous repair (HR). find that HR modifies pattern of repaired segment. also alters local histone H3 as well structure by inducing DNA-chromatin loops connecting 5′ 3′ ends gene. During a two-week period repair, transcription-associated demethylation promoted Base Excision Repair enzymes further DNA. Subsequently, genes display stable but diverse profiles. These profiles govern...
Abstract Histone methylation changes and formation of chromatin loops involving enhancers, promoters 3′ end regions genes have been variously associated with active transcription in eukaryotes. We studied the effect activation retinoic A receptor, at RARE–promoter CASP9 CYP26A1 genes, 15 45 min following RA exposure, we found that histone H3 lysines 4 9 are demethylated by lysine-specific demethylase, LSD1 JMJ-domain containing D2A. The action oxidase (LSD1) a dioxygenase (JMJD2A) presence...
Chronic obstructive pulmonary disease (COPD) is an inflammatory condition associated with abnormal immune responses, leading to airflow obstruction. Lungs of COPD subjects show accumulation proinflammatory T helper (Th) 1 and Th17 cells resembling that autoreactive responses. As regulatory (Treg) play a central role in the control autoimmune responses their generation function are controlled by adipocytokine leptin, we herein investigated association among systemic leptin overproduction,...
Recent studies have shown that hyperinsulinemia may increase the cancer risk. Moreover, many tumors demonstrate an increased activation of IR signaling pathways. Phosphatidylinositol 3-kinase (PI3K) is necessary for insulin action. In epithelial cells, which do not express GLUT4 and gluconeogenic enzymes, insulin-mediated PI3K regulates cell survival, growth, motility. Although involvement regulatory subunit (<mml:math xmlns:mml="http://www.w3.org/1998/Math/MathML"...
Easily accessible biomarkers in Huntington disease (HD) are actively searched. We investigated telomere length and DNA double‐strand breaks (histone variant pγ‐H2AX) as predictive peripheral blood mononuclear cells (PBMC) from 25 premanifest subjects, 58 HD patients with similar CAG expansion the huntingtin gene ( HTT ), 44 healthy controls (HC). PBMC pre‐HD groups showed shorter telomeres p < 0.0001) a significant increase of pγ‐H2AX compared to 0.0001). The levels correlated robustly...
Abstract We show that transcription induced by nuclear receptors for estrogen (E 2 ) or retinoic acid (RA) is associated with formation of chromatin loops juxtapose the 5’ end (containing promoter) enhancer and 3′ polyA addition site target gene. find three loop configurations which change as a function time after induction: 1. RA E -induced connect 5′ end, gene, are stabilized RNA early induction; 2. -independent whose stability does not require RNA; 3. Loops detected only treatment RNAse...
Abstract Background KRAS is the undisputed champion of oncogenes, and despite its prominent role in oncogenesis as mutated gene, mutation appears infrequent gliomas. Nevertheless, gliomas are considered KRAS-driven cancers due to essential mouse malignant gliomagenesis. Glioblastoma most lethal primary brain tumor, often associated with disturbed RAS signaling. For newly diagnosed GBM, current standard therapy alkylating agent chemotherapy combined radiotherapy. Cisplatin one effective...
Alexander disease (AD), a rare neurodegenerative disorder of the central nervous system, is characterized by accumulation cytoplasmic protein aggregates (Rosenthal fibers) composed glial fibrillary acidic (GFAP) and small heat‐shock proteins within astrocytes. To date, more than 40 different GFAP mutations have been reported in AD. The present study aimed at molecular diagnosis Italian patients suspected to be affected By analyzing gene 13 unrelated (eight with infantile form, two juvenile...
The aim of this study was to evaluate the treatment bovine semen with pan-caspase inhibitor benzyloxycarbonyl-Val-Ala-Asp-fluoromethyl ketone (Z-VAD-FMK), before or after freezing on quality. After initial assessment, sperm from 4 bulls were pooled (Experiment 1) and cryopreserved in BioXcell containing 0, 20 100 μM Z-VAD-FMK. thawing viability, motility, membrane integrity, as well DNA fragmentation ΔΨm evaluated. In Experiment 2, frozen/thawed incubated for 1 hr µM Z-VAD-FMK assessing Z...
Abstract Genome-wide methylation analysis is limited by its low coverage and the inability to detect single variants below 10%. Quantitative provides accurate information on extent of CpG dinucleotide, but it does not measure actual polymorphism profiles molecules. To understand DNA decode signatures before after damage repair, we have deep sequenced in bisulfite-treated a reporter gene undergoing site-specific homologous repair. In this paper, provide data generation, rationale for...
The estrogen receptor (ER) signaling regulates numerous physiological processes mainly through activation of gene transcription (genomic pathways). Caveolin1 (CAV1) is a membrane-resident protein that behaves as platform to enable different molecules and receptors for membrane-initiated pathways. CAV1 directly interacts with ERs allows their localization on membrane consequent ER-non-genomic Loss function common feature types cancers, including breast cancer. Two isoforms, CAV1α CAV1β,...
Abstract Osteopontin (OPN) is a phosphoglycoprotein secreted into the extracellular matrix upon liver injury, acting as cytokine stimulates deposition of fibrillary collagen in fibrogenesis. In livers mice subjected to bile duct ligation (BDL) and cultured activated hepatic stellate cells (HSCs), we show that OPN, besides being overexpressed, substantially phosphorylated by family with sequence similarity 20, member C (Fam20C), formerly known Golgi casein kinase (G‐CK), which exclusively...
Ce dossier s’inscrit dans le cadre d’une grande enquête collective développée à l’échelle internationale les dernières années, programme de recherche Babel Rome. La nature du monde et ses langues la Rome XVIe siècle.
Le 16e siècle romain correspond au déploiement long cours de sa connaissance et redéfinition l’Orient, à la croisée multiples savoirs pratiques, savantes ou ordinaires, qui redessinent les liens entre Antiquité monde contemporain. L’article en propose une lecture vise mettre lumière réarticulation des références spatio-temporelles l’Urbs, espace privilégié d’une confrontation l’Egypte, construite pivot l’Orient ancien, du Japon, expression moderne Orient extrême. Il fait l’hypothèse que...