A5101429501- Retinal Development and Disorders
- Pluripotent Stem Cells Research
- Retinal Diseases and Treatments
- Epigenetics and DNA Methylation
- RNA modifications and cancer
- Cancer, Hypoxia, and Metabolism
- Neuroscience and Neural Engineering
- Periodontal Regeneration and Treatments
- Cancer-related Molecular Pathways
- Cell death mechanisms and regulation
- Antibiotic Resistance in Bacteria
- Natural product bioactivities and synthesis
- Amino Acid Enzymes and Metabolism
- Infections and bacterial resistance
- Neuroscience and Neuropharmacology Research
- Bacterial biofilms and quorum sensing
- Protease and Inhibitor Mechanisms
- NF-κB Signaling Pathways
- GDF15 and Related Biomarkers
- Ubiquitin and proteasome pathways
- Computational Drug Discovery Methods
- Retinal and Macular Surgery
- Phosphodiesterase function and regulation
- Machine Learning in Bioinformatics
- Berberine and alkaloids research
University of Tübingen
2021-2025
Royal Pharmaceutical Society
2024
University College London
2024
Bernstein Center for Computational Neuroscience Tübingen
2022
Xian Central Hospital
2022
Soochow University
2010-2021
Sichuan University
2017-2021
Second Affiliated Hospital of Zhengzhou University
2012-2020
First Affiliated Hospital of Zhengzhou University
2014-2018
Rutgers, The State University of New Jersey
2015-2016
A goal in human embryonic stem cell (hESC) research is the faithful differentiation to given types such as neural lineages. During development, a basement membrane surrounds plate that forms tight, apico-basolaterally polarized epithelium before closing form tube with single lumen. Here we show three-dimensional epithelial cyst culture of hESCs Matrigel combined induction results quantitative conversion into neuroepithelial cysts containing Cells attain defined identity by 5 days. The...
Glutaminase (GLS) isoenzymes GLS1 and GLS2 are key enzymes for glutamine metabolism. Interestingly, display contrasting functions in tumorigenesis with elusive mechanism; promotes tumorigenesis, whereas exhibits a tumor-suppressive function. In this study, we found that but not binds to small GTPase Rac1 inhibits its interaction activators guanine-nucleotide exchange factors, which turn suppress cancer metastasis. This function of is independent glutaminase activity. Furthermore, decreased...
Age-related macular degeneration (AMD) is a major leading cause of visual impairment and blindness with no cure currently established. Cell replacement RPE discussed as potential therapy for AMD. Previous studies were performed in animal models severe limitations recapitulating the disease progression. In detail, we describe effect systemic injection sodium iodate mouse retina. We further evaluate usefulness this model to analyze cell-specific effects following transplantation human...
Background and Objective Tetrandrine (TET) is a bisbenzylisoquinoline alkaloid extracted from Stephania tetrandra Moore. Recent studies have suggested that TET can reduce the inflammatory response in microglia, but mechanisms remain unclear. The aim of this study to investigate whether inhibit lipopolysaccharide (LPS)-induced microglial activation clarify its possible mechanisms. Study Design/Materials Methods Cell viability assays cell apoptosis were used determine working concentrations...
ABSTRACT The axons of retinal ganglion cells (RGCs) form the optic nerve, which relays visual information to brain. RGC degeneration is root cause a variety blinding diseases linked nerve damage, including glaucoma, second leading blindness worldwide. underlying cellular mechanisms are largely unclear; yet, they have been connected excessive production signalling molecule nitric oxide (NO) by synthase (NOS). NO activates soluble guanylate cyclase (sGC), subsequently produces messenger cyclic...
DNA methyltransferase 1 (DNMT1), a large multidomain enzyme, is believed to be involved in the passive transmission of genomic methylation patterns via maintenance. Yet, molecular mechanism interaction networks underlying DNMT1 structures, dynamics, and its biological significance has yet fully characterized. In this work, we used an integrated computational strategy that combined coarse-grained atomistic simulations with coevolution information network modeling residue interactions for...
L2 β-lactamases, serine-based class A β-lactamases expressed by Stenotrophomonas maltophilia, play a pivotal role in antimicrobial resistance (AMR). However, limited studies have been conducted on these important enzymes. To understand the coevolutionary dynamics of β-lactamase, innovative computational methodologies, including adaptive sampling molecular simulations, and deep learning methods (convolutional variational autoencoders BindSiteS-CNN) explored conformational changes correlations...
Hereditary retinal degeneration (RD) is often associated with excessive cGMP signalling in photoreceptors. Previous research has shown that inhibition of cGMP-dependent protein kinase G (PKG) can reduce photoreceptor loss two different RD animal models. In this study, we identified a PKG inhibitor, the analogue CN238, which preserved viability and functionality rd1 rd10 mutant mice. Surprisingly, explanted retinae, CN238 also protected ganglion cells from axotomy-induced retrograde their...
this experimental design was based on HIPK3 to explore the pathogenesis of ESCC.RT-qPCR used detect expression CircHIPK3 and miR-599 in ESCC tissues cell lines.CCK-8, colony formation, flow cytometry transwell assay were effects tumor proliferation, apoptosis migration invasion. Target gene prediction screening, luciferase reporter assays validate downstream target genes miR-599.mRNA protein c-MYC detected by RT-qPCR Western blotting. The changes mice vivo experiments nude mice.HIPK3 highly...
Carcinoma-associated fibroblasts (CAFs) play a pivotal role in promoting the growth, invasion and metastasis of tumor cells. However, to date little is known about oncogenic mechanisms CAFs. This study aimed identify microenvironmental factors involved development progression directed by CAFs liver metastases. Tissue samples collected from 20 patients with colorectal metastases were used this study. Histological morphological characterization was performed using hybridization...
DNA methyltransferases (DNMTs) including DNMT1 are a conserved family of cytosine methylases that play crucial roles in epigenetic regulation. The versatile functions rely on allosteric networks between its different interacting partners, emerging as novel therapeutic targets. In this work, based the modeling structures DNMT1-ubiquitylated H3 (H3Ub)/ubiquitin specific peptidase 7 (USP7) complexes, we have used combination elastic network models, molecular dynamics simulations, structural...
How to improve the prediction accuracy of RNA secondary structure is currently a hot topic. The existing methods for single sequence do not fully consider folding diversity which may occur among RNAs with different functions or sources. This paper explores relationship between and accuracy, puts forward new method structure. Our research investigates following: 1. feature based on stochastic context-free grammar proposed. By using dimension reduction clustering techniques, some public data...