In this study, we investigated the effects of temozolomide (TMZ)/Poly (lactide-co-glycolide)(PLGA)/nano-hydroxyapatite microspheres on behavior U87 glioma cells. The were fabricated by “Solid/Water/Oil” method, and they characterized using X-Ray diffraction, scanning electron microscopy differential calorimetry. proliferation, apoptosis invasion cells evaluated MTT, flow cytometry assay Transwell assay. presence key invasive gene, αVβ3 integrin, was detected RT-PCR Western blot method. It...

Background: Gliomas are one of the most lethal cancers in human central nervous system. Despite clinical treatment advancements, prognosis patients with glioma remains poor. KDM2B is a histone lysine demethylase, which has been observed multiple tumors. But concrete role gliomas to be further illustrated. Methods: The expression was detected immunohistochemistry and Western blot assay. Furthermore, knockdown U87 U251 cell lines, proliferation capacity evaluated by viability assay, colon...

U87-derived stem-like cells (U87-SLCs) were cultured using serum-free stem cell media and identified by both biological behaviors markers. Tumor necrosis factor (TNF)-related apoptosis-inducing ligand (TRAIL) paclitaxel (PX), in combination or alone, was used to treat U87-MG human glioma (U87 cells) U87-SLCs. The results showed that TRAIL/PX cannot only synergistically inhibit U87 but also We observed a significantly higher apoptotic rate simultaneously treated with for 24 h compared either...

In recent years, the theory of brain glioma stem cells (BGSCs) has facilitated study gliomas. BGSCs have been accepted as origin gliomas and determine their biological features. Numerous efforts made to probe into characteristics behaviors BGSCs. However, culturing target remains essential first step for research on this study, we established a simplified procedure culture isolate from samples clinical patients. Samples 17 patients were included in processed grown serum-free cell media....

Gliomas are the most common and primary tumors of central nervous system in adults. Temozolomide (TMZ) is main drug used to treat glioma; however, prognosis remains poor for patients. Glioma stem cells (GSCs) thought enable glioma initiation evasion from immune surveillance; their immunogenicity can be determined by expression major histocompatibility complex (MHC)-I. The present study investigated effect TMZ on MHC-I GSCs. spheres were cultured serum-free medium containing epidermal growth...

In order to investigate the interplay between cells and TiO2 nanotube array, explore ability of sense size change in nano-environment, we reported on behavior glioma C6 array coatings terms proliferation apoptosis. The was obviously size-dependent coatings; caliber with 15 nm diameter provided effective spacing improve enhanced cellular activities. cells’ biological behaviors showed many similar tendencies phorocytes; matching degree geometry integrin defined that a optimal for inducing...

Membrane-associated guanylate kinase inverted repeat member 1 (MAGI1) acts as a tumor suppressor in variety of tumors; however, its expression and biological function glioma are still unknown.MAGI1 was examined by immunohistochemistry. In addition, overexpression MAGI1 U87 U373 cells, colony formation MTT assays were used to evaluate cell proliferation, Transwell determine migration invasion, xenograft model established using cells the effect vivo. Western blot analyze Akt, MMP2, MMP9...

Glioblastoma multiforme (GBM) is one of the most frequent primary malignancies brain.Although treatment strategy has significantly improved, patient prognosis remains poor.In vitro studies have shown that right open reading frame kinase 1/protein B (RIOK1-AKT) signaling pathway plays an important role in malignant phenotype glioma cells.This study aimed to investigate co-expression RIOK1 and ATK tissues its clinical significance.Compared with normal tissues, AKT1 expression were upregulated...

The effect of scinderin (SCIN) on cancer progression has been studied, but its role in glioma remains unknown. This study describes the value SCIN for diagnosis, prognosis, and treatment glioma.The expression was analyzed using GEPIA, Oncomine, cBioPortal, CGGA databases. GO/KEGG enrichment analysis similar genes to were performed R software package, protein-protein interaction (PPI) network by STRING GeneMANIA correlations mRNA between MMP2/9 TCGA glioma. Simultaneously, TISIDB TIMER...

Glioma patients receiving therapy are at a high risk of relapse and rapid progression and, thus, more effective treatments required. The aim the present study was to determine suppressive role miR‑489 as an alternative therapeutic target for preventing glioma progression. results demonstrated that with relatively lower levels expression had favorable clinical outcomes. Furthermore, inversely correlated p21‑activated kinase 5 (PAK5) mRNA in specimens. A dual luciferase reporter assay revealed...

Abstract: Bilateral basal ganglia hemorrhages are extremely rare and have very poor prognosis. We describe the case of a 52-year-old woman with history hypertension who experienced bilateral hemorrhages. performed hematoma aspiration by minimally invasive surgery via frontal temporal puncture points. discuss surgical procedure review relevant literature pertaining to underlying causes complications similar cases. Keywords: preexisting hypertension, intracranial hemorrhage, procedure,...

Solitary fibrous tumor (SFT) of the central nervous system is rare. It predominantly benign and rarely malignant. There no established standardized treatment regimen for malignant intracranial SFTs.We present a rare case SFT in 9-year-old girl with space-occupying effect frontal-parietal lobes. She underwent craniotomy, mass was resected. Immunohistochemistry examination specimen showed that Ki-67 proliferation index staining highly positive 80% cells. Whole exome sequencing surgical tissue...

Objective To investigate the expression of interleukin-6 in glioma stem cells (GSCs) and its role invasion.Method GSCs from eight surgical specimens were cultured identified.Real-time RT-PCR Enzyme-Linked Immunosorbent Assay used to measure compare levels IL-6 corresponding primary (PGCs).Subsequently,IL-6 antibody or exogenous was a Matrigel-invasion assay effects on PGCs invasiveness then determined.Results Paired t-test showed that mRNA protein significantly higher than identical tumor (t...

Objective To determine the mRNA expression of transforming growth factor β2 (TGF-β2) in human brain glioma stem cells (BGSCs),and investigate its effect onimmunotherapy glioma. Methods BGSCs were grown from 8 specimens resected October 2008 to January 2009,then,they isolated and identified.The expressions ofCD133,GFAP TU-20 tumor cell spheres detected by immunofluorescence staining; TGF-β2 was RT-PCR,and then,this result compared with that respective differentiated cells. Results Positive...