Abstract Background Schwann cell-like cells (SCLCs), differentiated from mesenchymal stem cells, have shown promising outcomes in the treatment of peripheral nerve injuries preclinical studies. However, certain clinical obstacles limit their application. Hence, primary aim this study was to investigate role exosomes derived SCLCs (SCLCs-exo) regeneration. Methods were human amniotic (hAMSCs) vitro and validated by immunofluorescence, real-time quantitative PCR western blot analysis. Exosomes...

Abstract Background Wound healing is a complex pathophysiological process that involves variety of cells and cytokines. In this study, we found local injection human amnion mesenchymal stem into wounds in rats could promote wound healing. Therefore, hypothesized the exosomes contain substances regulate migration epidermal cells. It has been reported miR-135a involved cell transformation. However, there have no reports its function skin Methods To test hypothesis, injected overexpressing...

Background Diabetic foot ulcer (DFU) is a severe complication that occurs in patients with diabetes and primary factor necessitates amputation. Therefore, the occurrence progression of DFU must be predicted at an early stage to improve patient prognosis outcomes. In this regard, emerging evidence suggests inflammation-related markers play significant role DFU. One such potential marker, monocyte-lymphocyte ratio (MLR), has not been extensively studied relation This study aimed define...

Acinetobacter baumannii (A. baumannii) has emerged as one of the most troublesome pathogens in health care institutions. A. can cause a wide range diseases humans, including pneumonia and septicemia. Phage therapy drawn great interest from medical researchers potential way to control infections by antibiotic-resistant . Using pandrug-resistant clinical isolate AB ZY 9 an indicator, we isolated lytic phage Abp9 hospital sewage. belongs myoviridae family shows wider host 12%. contains linear...

Despite being an attractive cell type for mesenchymal stem (MSC) transplantation therapy wound healing, human adipose-derived cells (hADSCs) from diabetes mellitus (DM) patients result in remarkable retention of activity due to diabetes-induced glucolipotoxicity.We explored the effect and medium containing AGEs on activity, phenotype, multipotency, angiogenic potential, therapeutic hADSCs.Then, miRNA-1248 was selected by miRNA microarray analysis further study core molecular pathways that...

Abstract Background Chronic refractory wounds easily relapse and seriously affect the patients’ quality of life. Previous studies have shown that stromal vascular fraction gel (SVF-gel) significantly promotes early healing chronic wounds; however, mechanisms SVF-gel function per se remain unclear, a long-term follow-up is lacking. This study aims to explore promoting follow up efficacy SVF-gel. Methods Autologous transplantation was performed in 20 patients with (from March 2016 September...

Objective: This study evaluated the efficacy of various local management strategies for diabetic foot ulcers (DFUs). Background: Several surgical and non-surgical interventional approaches are available treatment DFUs. The comparative effectiveness different treatments is unknown, it remains unclear which approach optimal choice DFUs due to limited direct comparisons. Methods: We did a systematic review meta-analysis select management. searched Medline, Embase, Web Science,...

G protein-coupled receptor 75 (GPR75), a novel member of the rhodopsin-like (GPCR) family, has been identified across various tissues and organs, where it contributes to biological regulation disease progression. Recent studies suggest potential interactions between GPR75 ligands such as 20-hydroxyeicosatetraenoic acid (20-HETE) C-C motif chemokine ligand 5 (CCL5/RANTES); however, its definitive endogenous remains unidentified, is currently classified an orphan by International Union Basic...

Mesenchymal stem cells (MSCs) possess a capacity to enhance cutaneous wound healing that is well characterized. However, the therapeutic effect of MSCs appears be limited. Modifying MSC target genes increase necessary biological effects promising strategy for therapy. Interleukin-10 (IL-10) an anti-inflammatory cytokine has on healing. In this study, we modified human amniotic mesenchymal (hAMSCs) using recombinant lentiviral vectors expressing IL-10 and evaluated hAMSCs-IL-10 in We...

Diabetic foot ulcer (DFU) is frequently difficult to heal and finally leads amputation, resulting in high mortality rate diabetic patients. To date, effective optimal therapies are still lacking. This study aims investigate the efficacy of integrated surgical wound treatment (ISWT) mode on wound.From January 2021 December 2021, 13 patients with Wagner grade 3 4 were treated ISWT mode, which combined TTT technique debridement, induced membrane technique, vacuum sealing drainage (VSD) skin...

Adipose-derived stem cell- (ADSC-) based regenerative medicine has expanded to include the treatment of hair loss. However, cell therapy remains a relatively recent technique, and reports its use for treating alopecia are rare. ADSCs exert biological functions via paracrine actions various growth factors cytokines. Conditioned medium from (ADSCs-CM) is cell-free suspension rich in cytokines that demonstrated significant role stimulating growth, with encouraging outcomes terms regeneration...

This study aimed to reveal the distribution pattern of antebrachial cutaneous nerves and provide a morphological basis for sensory reconstruction during flap transplantation. Forearm specimens containing skin subcutaneous fat were obtained from 24 upper extremities 12 adult cadavers. Cutaneous visualized using modified Sihler's staining. Then data was used show innervation area forearm nerve. The anterior branch lateral nerve innervates 26% medial forearm; posterior 38.21% 24.46% forearm....

Abstract Diabetic foot ulcers (DFUs) present significant challenges due to their associated amputation rates, mortality, treatment complexity and excessive costs. Our earlier work introduced a wound surgical integrated (WSIT) for DFUs, yielding promising outcomes. This study focuses on specific WSIT protocol employing antibiotic‐loaded bone cement (ALBC) in the first Stage, free vastus lateralis muscle‐sparing (VLMS) flaps split‐thickness skin grafts (STSGs) second stage repair...

The use of Schwann cell-like cells (SCLCs) derived from stem has been introduced as an effective strategy for promoting peripheral nerve regeneration (PNR). However, molecular mechanisms underlying therapeutic transplantation SCLCs PNR are often ignored. To explore the potential treatment sciatic never injury and investigate molecule mechanisms. differentiated human amniotic mesenchymal (hAMSCs) specific markers were detected. transplanted into injured sites a rat model injury, functional...

Objective Burn wounds are a global public health problem, and large number of casualties caused by burns each year. In this study, we explored the epidemiological characteristics associated with factors affecting length hospital stay (LOS) in children adults burn wounds. Methods Records patients Affiliated Hospital Zunyi Medical University from January 1, 2014 to August 31, 2016 were retrieved. Information on demographic characteristics, mechanism burns, first treatment received, clinical...

Pressure injury (PI) affects quality of life and results in economic social burdens. Local transplantation human adipose-derived stem cells (ASCs) is considered an effective treatment. However, ASC suspension alone vulnerable to the immune system a shortened cell survival. There increasing evidence synergistic effect platelet-rich plasma (PRP) combined with ASCs on wound healing. This study investigated effectiveness, synergy, mechanism healing following local injection PRP rodent PI model....

To investigate the feasibility and effectiveness of antibiotic bone cement directly inducing skin regeneration technology in repairing wound lateral toe flap donor area.

Abstract Anterolateral thigh (ALT) free flaps are widely used for reconstruction, but poor sensory recovery of the flap tissue can cause unsatisfactory outcomes and function. Adipose-derived mesenchymal stem cells (ADSCs) promote neural regeneration, clinical use stem-cell therapy has been limited by lack regulatory approval. Nanofat is an autologous product that prepared mechanically from harvested fat. It enriched in ADSCs does not contain any exogenous substances. The developmental adult...