Autologous fat grafting (AFG) often needs multiple sessions due to low volume retention. Young adipose tissue demonstrates a more pronounced therapeutic effect; thus, the cryopreservation of young origin is particularly crucial. This study investigated protective effect new solution combining trehalose, glycerol, and metformin on tissue. initially examined various concentrations (0, 1, 2, 4, 8 mM) oxidative damage in identify optimal concentration. Subsequently, 1.5 mL fresh human was...

Given the widespread presence of spirocyclic scaffolds in natural products and pharmacologically relevant synthetic compounds, development efficient methodologies for their construction holds great importance medicinal chemistry pharmaceutical research. In this study, a general photochemical dearomative spirocyclization nonactivated arenes has been established. The key to method lies ability amine-borane radicals activate aryl bromides iodides via halogen-atom transfer, thereby allowing...

Abstract Background Schwann cell-like cells (SCLCs), differentiated from mesenchymal stem cells, have shown promising outcomes in the treatment of peripheral nerve injuries preclinical studies. However, certain clinical obstacles limit their application. Hence, primary aim this study was to investigate role exosomes derived SCLCs (SCLCs-exo) regeneration. Methods were human amniotic (hAMSCs) vitro and validated by immunofluorescence, real-time quantitative PCR western blot analysis. Exosomes...

Stem cell therapy is considered as the most promising treatment for chronic wounds. Extracellular matrix/stromal vascular fraction gel (ECM/SVF gel), an adipose-derived stem cell-based cytotherapy, has shown healing potential in experimental wounds animal models. However, effects of ECM/SVF on human have not been investigated. The aim present study to investigate therapeutic effect Autologous was prepared and used treat patients with clinics, negative pressure wound positive control. Wound...

Taking advantage of cellular paracrine mechanisms, the secretome adipose-derived stem cells (ADSCs) and adipose tissue has been demonstrated to induce repair regeneration in various ischemic impaired conditions. However, these cell-based therapies have hindered by issues, such as inherent safety cost-efficiency for clinical applications. In this study, we prepared a liquid cell-free extract from human [adipose (ALE)] evaluated its potential therapeutic efficacy. ALE was subcutaneous using...

Conventional treatments for Raynaud's phenomenon (RP) often show limited effectiveness due to their inability address both vascular and inflammatory aspects. This study evaluates the combination of high-density fat grafting (HDFG) with botulinum toxin A (BTX-A) treating RP. Eleven patients 20 affected hands diagnosed RP were recruited randomly assigned receive either HDFG combined BTX-A (intervention group, n = 11) or alone (control 9). Efficacy was assessed using Visual Analog Scale (VAS)...

Despite being an attractive cell type for mesenchymal stem (MSC) transplantation therapy wound healing, human adipose-derived cells (hADSCs) from diabetes mellitus (DM) patients result in remarkable retention of activity due to diabetes-induced glucolipotoxicity.We explored the effect and medium containing AGEs on activity, phenotype, multipotency, angiogenic potential, therapeutic hADSCs.Then, miRNA-1248 was selected by miRNA microarray analysis further study core molecular pathways that...

Abstract Background Chronic refractory wounds easily relapse and seriously affect the patients’ quality of life. Previous studies have shown that stromal vascular fraction gel (SVF-gel) significantly promotes early healing chronic wounds; however, mechanisms SVF-gel function per se remain unclear, a long-term follow-up is lacking. This study aims to explore promoting follow up efficacy SVF-gel. Methods Autologous transplantation was performed in 20 patients with (from March 2016 September...

Adipose-derived stem cells (ASCs) possess a well-characterized capacity to enhance cutaneous wound healing. However, many controversies exist regarding ASCs from diabetic patients (dASCs). No report exists on the administration of dASCs for treatment pressure ulcers. The aim this study was compare properties and nondiabetic (nASCs). In addition, we studied if enhanced ulcer healing in rodent model investigated underlying mechanisms. We found similar expression cell surface markers...

Mesenchymal stem cells (MSCs) possess a capacity to enhance cutaneous wound healing that is well characterized. However, the therapeutic effect of MSCs appears be limited. Modifying MSC target genes increase necessary biological effects promising strategy for therapy. Interleukin-10 (IL-10) an anti-inflammatory cytokine has on healing. In this study, we modified human amniotic mesenchymal (hAMSCs) using recombinant lentiviral vectors expressing IL-10 and evaluated hAMSCs-IL-10 in We...

Diabetic foot ulcer (DFU) is frequently difficult to heal and finally leads amputation, resulting in high mortality rate diabetic patients. To date, effective optimal therapies are still lacking. This study aims investigate the efficacy of integrated surgical wound treatment (ISWT) mode on wound.From January 2021 December 2021, 13 patients with Wagner grade 3 4 were treated ISWT mode, which combined TTT technique debridement, induced membrane technique, vacuum sealing drainage (VSD) skin...

Secondary lymphedema is a chronic, disabling disease affecting more than 50% of patients with cancer and lacking effective pharmacologic treatment even for early to middle stages. Metformin reportedly exerts anti-inflammatory antifibrotic effects safe, minimal side effects. The authors investigated the role metformin in mouse models examined underlying molecular mechanisms.

Abstract Conditioned medium (CM) is a new treatment modality in regenerative medicine and has shown successful outcome wound healing. We recently introduced extracellular matrix/stromal vascular fraction gel (ECM/SVF‐gel), an adipose‐derived stem cell adipose native matrix‐enriched product for cytotherapy. This study aimed to evaluate the effect of CM from ECM/SVF‐gel (Gel‐CM) on healing compared with conventional tissue (Adi‐CM) (SVF‐CM). In vitro three CMs keratinocytes fibroblasts was...

Acinetobacter baumannii has become one of the most challenging conditional pathogens in health facilities. It causes various infectious diseases humans, such as wound or urinary tract infections and pneumonia. Phage therapy been used an alternative strategy for antibiotic-resistant A. approved by several governments. Previously, we have reported two potential phage candidates, Abp1 Abp9, both which are narrow-host-range phages. In present study, screened isolated 22 bacteriophages from...

Adipose-derived stem cell- (ADSC-) based regenerative medicine has expanded to include the treatment of hair loss. However, cell therapy remains a relatively recent technique, and reports its use for treating alopecia are rare. ADSCs exert biological functions via paracrine actions various growth factors cytokines. Conditioned medium from (ADSCs-CM) is cell-free suspension rich in cytokines that demonstrated significant role stimulating growth, with encouraging outcomes terms regeneration...

The use of Schwann cell-like cells (SCLCs) derived from stem has been introduced as an effective strategy for promoting peripheral nerve regeneration (PNR). However, molecular mechanisms underlying therapeutic transplantation SCLCs PNR are often ignored. To explore the potential treatment sciatic never injury and investigate molecule mechanisms. differentiated human amniotic mesenchymal (hAMSCs) specific markers were detected. transplanted into injured sites a rat model injury, functional...

Aim: We investigated the healing effects of conditioned medium (CM) derived from a physiological 3D culture system engineered to use an extracellular matrix/stromal vascular fraction (SVF) gel enriched for adipose on diabetic wounds in rats. This CM (Gel-CM) was compared with that 2D used SVF cells (SVF-CM). Materials & methods: Keratinocytes, fibroblasts and were treated Gel-CM SVF-CM, cytokine levels types quantified. Results: Proliferation migration keratinocytes significantly higher...

Background The oblique branch of the lateral circumflex femoral artery is considered an alternative vascular pedicle anterolateral thigh (ALT) flap. However, has several advantages over descending branch. Methods A total 61 consecutive ALT free flaps were harvested according to Yu's ABC system. Vascular dimensions and length, course, perforator origins, flap harvest time with or recorded. We classified branches into three types origin B. Type I those where B originated from II without III...