A5032006991- Neonatal Respiratory Health Research
- Inhalation and Respiratory Drug Delivery
- Respiratory Support and Mechanisms
- Respiratory viral infections research
- Immune Response and Inflammation
- Chronic Obstructive Pulmonary Disease (COPD) Research
- Antimicrobial Peptides and Activities
- Glycosylation and Glycoproteins Research
- Pediatric health and respiratory diseases
- Neuroscience of respiration and sleep
- Galectins and Cancer Biology
- Cancer-related gene regulation
- Helicobacter pylori-related gastroenterology studies
- Congenital Diaphragmatic Hernia Studies
- Influenza Virus Research Studies
- Asthma and respiratory diseases
- Air Quality and Health Impacts
- Toxin Mechanisms and Immunotoxins
- Neonatal and Maternal Infections
- Viral Infections and Outbreaks Research
- Infant Nutrition and Health
- Cystic Fibrosis Research Advances
- Microtubule and mitosis dynamics
- Immunodeficiency and Autoimmune Disorders
- Bacterial Genetics and Biotechnology
University College London
2019-2025
University of Southampton
2012-2025
London Women's Clinic
2024-2025
University College Hospital
2021-2024
Southampton General Hospital
2010-2020
NIHR Southampton Respiratory Biomedical Research Unit
2013-2018
National Institute for Health Research
2014-2018
University Hospital Southampton NHS Foundation Trust
2014-2018
Wellcome Trust
2014
Massachusetts General Hospital
2008
Abstract Lung surfactant protein-D (SP-D), a collectin mainly produced by alveolar type II cells, initiates the effector mechanisms of innate immunity on binding to microbial carbohydrates. A panel mRNAs from human tissues was screened for SP-D mRNA RT-PCR. The lung main site synthesis, but transcripts were readily amplified trachea, brain, testis, salivary gland, heart, prostate kidney, and pancreas. Minor sites synthesis uterus, small intestine, placenta, mammary stomach. sequence derived...
Surfactant protein D (SP-D) is an oligomeric C type lectin that promotes phagocytosis by binding to microbial surface carbohydrates. A 340-kDa glycoprotein (gp-340) has been shown bind SP-D in the presence of calcium but does so independently carbohydrate recognition. This exists both a soluble form and association with membranes alveolar macrophages. The primary structure gp-340 established molecular cloning, which yielded 7,686-bp cDNA sequence encoding polypeptide chain 2,413 amino acids....
Trefoil factors (TTFs) are small, compact proteins coexpressed with mucins in the gastrointestinal tract. Three trefoil known mammals: TFF1, TFF2, and TFF3. They implicated to play diverse roles maintenance repair of channel. We compared expression pattern three analyzing mRNA from a panel 20 human tissues by conventional reverse transcriptase (RT) PCR and, addition, real-time PCR. These findings were supported immunohistochemical analysis paraffin-embedded using rabbit polyclonal antibodies...
Over the last four decades, H3N2 subtype influenza A viruses have gradually acquired additional potential sites for glycosylation within globular head of hemagglutinin (HA) protein. Here, we examined biological effect on virulence viruses. We created otherwise isogenic reassortant by site-directed mutagenesis that contain and in naïve BALB/c, C57BL/6, surfactant protein D (SP-D)-deficient mice. The introduction was consistent with sequence acquisition over past 40 years, beginning two 1968...
Abstract Surfactant protein D (SP-D) plays important roles in the host defense against infectious microorganisms and regulating innate immune response to a variety of pathogen-associated molecular pattern. SP-D is mainly expressed by type II cells lung, but generally found on epithelial surfaces serum. Genotyping for three single-nucleotide variations altering amino acids mature codon 11 (Met11Thr), 160 (Ala160Thr), 270 (Ser270Thr) gene was performed related levels Individuals with...
Lung surfactant protein A (SP-A) is a collectin produced by alveolar type II cells and Clara cells. It binds to carbohydrate structures on microorganisms, initiating effector mechanisms of innate immunity modulating the inflammatory response in lung. Reverse transcriptase-polymerase chain reaction was performed panel RNAs from human tissues for SP-A mRNA expression. The lung main site synthesis, but transcripts were readily amplified trachea, prostate, pancreas, thymus. Weak expression...
Surfactant protein D (SP-D) is primarily expressed in the lungs and modulates pro- anti-inflammatory processes to toxic challenge, maintaining lung homeostasis. We investigated interaction between NPs SP-D subsequent uptake by cells involved immunity. Dynamic light scattering (DLS) scanning electron microscopy (SEM) measured NP aggregation, particle size charge native human (NhSP-D) recombinant fragment (rfhSP-D). aggregated NPs, especially following addition of calcium. Immunohistochemical...
The formation of protein coronae on nanoparticles (NPs) has been investigated almost exclusively in serum, despite the prevailing route exposure being inhalation airborne particles. In addition, an increasing number nanomedicines, that exploit airways as site delivery, are undergoing medical trials. An understanding effects NPs is therefore required. To further this field, we have described corona formed polystyrene (PS) particles with different surface modifications and titanium dioxide...
The protein deleted in malignant brain tumors (DMBT1) and the trefoil factor (TFF) proteins have all been proposed to roles epithelial cell growth differentiation shown be up regulated inflammatory bowel diseases. A panel of monoclonal antibodies was raised against human DMBT1gp340. Analysis lung washings colon tissue extracts by Western blotting unreduced state, two (Hyb213-1 Hyb213-6) reacted with a double band 290 kDa lavage. Hyb213-6, addition, 270 extract while Hyb213-1 showed no...
Pulmonary epithelial cell responses can enhance type 2 immunity and contribute to control of nematode infections. An important product is the collectin Surfactant Protein D (SP-D). We found that SP-D concentrations increased in lung following Nippostrongylus brasiliensis infection; this increase was dependent on key components immune response. carried out loss gain function studies establish if required for optimal parasite. N. infection SP-D-/- mice resulted profound impairment host innate...
The role of surfactant proteins A and D (SP-A SP-D) in lung clearance translocation to secondary organs inhaled nanoparticles was investigated by exposing SP-A SP-D knockout (AKO DKO) wild type (WT) mice nose-only for 3 hours an aerosol 20 nm gold (AuNPs). Animals were euthanised at 0-, 1-, 7- 28-days post-exposure. Analysis inductively coupled plasma mass spectrometry (ICP-MS) the liver kidneys showed that extrapulmonary below limits detection. Imaging lungs laser ablation ICP-MS confirmed...
Abstract CRP‐ductin is a protein expressed mainly by mucosal epithelial cells in the mouse. Sequence homologies indicate that mouse homologue of human gp‐340, glycoprotein agglutinates microorganisms and binds lung collectin surfactant protein‐D (SP‐D). Here we report purified SP‐D calcium‐dependent manner binding not inhibited maltose. The same properties have previously been observed for gp‐340 SP‐D. also showed to both gram‐positive ‐negative bacteria. A polyclonal antibody raised against...
Surfactant protein D (SP-D) is an important innate immune defense molecule that mediates clearance of pathogens and modulates the inflammatory response. Moreover, SP-D involved in lipid homeostasis, pulmonary accumulation phospholipids has previously been observed SP-D-deficient ( Spd−/−) mice. Atherogenesis involves both inflammation deposition, we investigated role development atherosclerosis. synthesis was localized to vascular endothelial cells. Atherosclerotic lesion areas were 5.6-fold...
Abstract Deleted in Malignant Brain Tumors 1 ( DMBT1 ) at chromosome region 10q25.3–q26.1 has been proposed as a candidate tumor‐suppressor gene for brain, digestive tract, and lung cancer. Recent studies on its expression cancer have led to divergent results raised controversial discussion. Moreover, implicated with epithelial protection the respiratory tract. We thus wondered how loss of could be related carcinogenesis lung. To address these issues, we investigated location normal By...
Surfactant Protein D (SP-D) is an oligomerized C-type lectin molecule with immunomodulatory properties and involvement in lung surfactant homeostasis the respiratory tract. SP-D binds to enveloped viruses, influenza A virus syncytial inhibits their replication vitro vivo. has been shown bind HIV via envelope protein gp120 inhibit infectivity vitro. Here we show that different strains of (BaL IIIB) binding occurs at both pH 7.4 5.0 resembling physiological relevant values found body female...
The lung provides the main route for nanomaterial exposure. Surfactant protein A (SP-A) is an important respiratory innate immune molecule with ability to bind or opsonise pathogens enhance phagocytic removal from airways. We hypothesised that SP-A, like surfactant D, may interact inhaled nanoparticulates, and this interaction will be affected by nanoparticle (NP) surface characteristics. In study, we characterise of SP-A unmodified (U-PS) amine-modified (A-PS) polystyrene particles varying...
Respiratory syncytial virus (RSV) is the leading cause of bronchiolitis and hospitalisation infants in developed countries. Surfactant protein A (SP-A) an important innate immune molecule, localized pulmonary surfactant. SP-A binds to carbohydrates on surface pathogens a calcium-dependent manner enable neutralisation, agglutination clearance including RSV. forms trimeric units further oligomerises through interactions between its N-terminal domains. Whilst recombinant fragment closely...