This investigation examined the influence of number repetitions per set on power output and muscle metabolism during leg press exercise. Six trained men (age 34 ± 6 yr) randomly performed either 5 sets 10 (10REP), or (5REP) bilateral exercise, with same initial load rest intervals between sets. Muscle biopsies (vastus lateralis) were taken before first set, after final Compared 5REP, 10REP resulted in a markedly greater decrease (P<0.05) output, PCr ATP content, higher levels lactate IMP....

Diabetes is frequently diagnosed late, when the development of complications almost inevitable, decreasing quality life patients. However, early detection affected individuals would allow implementation timely and effective therapies.Here we set to describe profile circulating microRNAs (miRNAs) in prediabetic patients with intention identifying novel diagnostic therapeutic tools.We used real-time RT-PCR measure abundance 176 miRNAs serum a cohort 92 control either impaired fasting glucose...

The effect of the distribution rest periods on efficacy interval sprint training is analysed. Ten male subjects, divided at random into two groups, performed distinct incremental protocols, in which muscle load was same (14 sessions), but varied. `short programme' group (SP) trained every day for 2 weeks, while `long (LP) over a 6‐week period with 2‐day following each session. volunteers 30‐s supramaximal cycling test cycle ergometer before and after training. Muscle biopsies were obtained...

Gorostiaga, EM, Navarro-Amézqueta, I, Calbet, JAL, Sánchez-Medina, L, Cusso, R, Guerrero, M, Granados, C, González-Izal, Ibáñez, J, and Izquierdo, M. Blood ammonia lactate as markers of muscle metabolites during leg press exercise. J Strength Cond Res 28(10): 2775–2785, 2014—To examine whether blood concentrations can be used to estimate the functional state contractile machinery with regard adenosine triphosphate (ATP) levels Thirteen men (age, 34 ± 5 years; 1 repetition maximum strength...

The purpose of the present study was to investigate effects 8 months a specific and controlled sprint training programme on three groups young athletes (two males one females). Biopsies vastus lateralis were taken before after period training. type percentage diameter fibres, as well glycogen content activities enzymes metabolism (glycogen synthase phosphorylase), glycolysis (phosphofructokinase, pyruvate kinase, aldolase lactate dehydrogenase), oxidative (succinate de‐hydrogenase) creatine...

The extremely high energy demand elicited by sprint exercise is satisfied an increase in O 2 consumption combined with a glycolytic rate, leading to marked lactate accumulation, increased AMP-to-ATP ratio, and reduced NAD + /NADH.H muscle pH, which are accompanied Thr 172 AMP-activated protein kinase (AMPK)-α phosphorylation during the recovery period mechanism not fully understood. To determine role played reactive nitrogen oxygen species (RNOS) on -AMPKα response cycling exercise, nine...

The comparability and quality of UNESCO's international education statistics have been questioned since the end 1980s, when other agencies started producing their own indicators on education. This evolution coincides with new strategic role given to in national economic programmes, marks transition from era development that globalization. According OECD or World Bank, more emphasis should be placed outputs than measurement mass Moreover, used not only compare characteristics systems but also...

Roser Cussó,

Purpose: To assess early changes in muscle function and hypertrophy, measured as increases cross-sectional areas (CSAs) total volume, over a 4 weeks inertial resistance training (RT) program. Methods: Ten young RT-naive volunteers (age 23.4 ± 4.1 years) underwent 10 sessions (2-3 per week) consisting of five sets flywheel squats (moment inertia 900 kg⋅cm2). Magnetic resonance imaging (MRI) scans both thighs were performed before (PRE), after 2 (IN) (POST) to compute individual volumes...

Glycogen metabolism has been the subject of extensive research, but mechanisms by which it is regulated are still not fully understood. It well accepted that rate-limiting enzymes in glycogenesis and glycogenolysis glycogen synthase (GS) phosphorylase (GPh), respectively. Both reversible phosphorylation allosteric effectors. However, evidence literature indicates changes muscle GS GPh intracellular distribution may constitute a new regulatory mechanism metabolism. Already 1960s, was proposed...

Information about anaerobic energy production and mechanical efficiency that occurs over time during short-lasting maximal exercise is scarce controversial. Bilateral leg press an interesting muscle contraction model to estimate because it largely differs from the models used until now. This study examined changes in metabolite concentration power output first second half of a set 10 repetitions failure (10RM) bilateral exercise. On two separate days, biopsies were obtained vastus lateralis...

As metabolic changes in human skeletal muscle after long-term (simulated) spaceflight are not well understood, this study examined the effects of microgravity, with and without concurrent resistance exercise, on oxidative glycolytic capacity. Twenty-one men were subjected to 84 days head-down tilt bed rest (BRE; n = 9) or (BR; 12) flywheel exercise. Activity gene expression glycogen synthase, phosphorylase (GPh), hexokinase, phosphofructokinase-1 (PFK-1), citrate synthase (CS), as succinate...

Mammalian skeletal muscle expresses GLUT-4 and GLUT-1 glucose transporters. Here, we have investigated whether expression is regulated in by contractile activity. mRNA levels were high at days 16 17 of fetal life decreased markedly 19 21. In contrast, clearly detectable day 21 life, they increased progressively during postnatal life. The timing data for induction repression suggest that these processes are related to innervation. adult rat rabbit tibialis anterior after severage peroneal...

The diagnosis of muscular lesions suffered by athletes is usually made clinical criteria combined with imaging the lesion (ultrasonography and/or magnetic resonance) and blood tests to detect presence non-specific muscle markers. This study was undertaken evaluate injury fast slow-twitch fibres using specific markers for these fibres.Blood samples were obtained from 51 non-sports people 38 sportsmen skeletal injury. Western analysis performed determine slow myosin creatine kinase (CK)...

Background Glycogen-depleting exercise can lead to supercompensation of muscle glycogen stores, but the biochemical mechanisms this phenomenon are still not completely understood. Methods Using chronic low-frequency stimulation (CLFS) as an model, tibialis anterior rabbits was stimulated for either 1 or 24 hours, inducing a reduction in 90% and 50% respectively. Glycogen recovery subsequently monitored during hours rest. Results In muscles hour, recovered basal levels rest period. However,...

Muscle damage induced by inertial exercise performed on a flywheel device was assessed through the serum evolution of muscle enzymes, interleukin 6, and fiber type‐specific sarcomere proteins such as fast myosin ( FM ) slow SM ). We hypothesized that model could be constructed measuring concentration following exercise, according to their molecular weight compartment in which they are located. Moreover, , type fibers affected assessed. Serum profiles were registered before 24, 48, 144 h...

Glucose 1,6-bisphosphate, fructose 2,6-bisphosphate, glycogen, lactate and other glycolytic metabolites were measured in rat gastrocnemius muscle, which was electrically stimulated situ via the sciatic nerve. Both frequency duration of stimulation varied to obtain different rates glycolysis. There no apparent relationship between 2,6-bisphosphate content accumulation contracting muscle. In contrast, glucose 1,6-bisphosphate increased with concentration during contraction. It is suggested...