A5049446238- Adipose Tissue and Metabolism
- Regulation of Appetite and Obesity
- Exercise and Physiological Responses
- Estrogen and related hormone effects
- Muscle metabolism and nutrition
- Growth Hormone and Insulin-like Growth Factors
- Bioactive Compounds and Antitumor Agents
- Chronic Myeloid Leukemia Treatments
- Metabolism, Diabetes, and Cancer
- Synthesis and biological activity
- Liver Disease Diagnosis and Treatment
- Menopause: Health Impacts and Treatments
- Muscle Physiology and Disorders
- Cytokine Signaling Pathways and Interactions
- Adipokines, Inflammation, and Metabolic Diseases
- Cardiovascular and exercise physiology
- Stress Responses and Cortisol
- Cancer, Lipids, and Metabolism
- Receptor Mechanisms and Signaling
- Lipid metabolism and disorders
- Hormonal Regulation and Hypertension
- Cancer, Hypoxia, and Metabolism
- Sports Performance and Training
- High Altitude and Hypoxia
- Neurobiology and Insect Physiology Research
Universidad de Las Palmas de Gran Canaria
2014-2024
Instituto de Investigaciones Biomédicas Sols-Morreale
2014-2023
Universidad Autónoma de Madrid
2022-2023
Consejo Superior de Investigaciones Científicas
2022-2023
Canary Institute of Cancer Research
2014-2018
Institute for Research in Biomedicine
2016
Departamento de Educación
2013
Universidad de La Laguna
2001-2003
Texas Biomedical Research Institute
2000
To elucidate the molecular mechanisms behind physical inactivity–induced insulin resistance in skeletal muscle, 12 young, healthy male subjects completed 7 days of bed rest with vastus lateralis muscle biopsies obtained before and after. In six subjects, were taken from both legs after a 3-h hyperinsulinemic euglycemic clamp performed 3 h 45-min, one-legged exercise. Blood samples one femoral artery veins during clamp. Glucose infusion rate leg glucose extraction lower than rest. This...
AMP-activated protein kinase (AMPK) is a major mediator of the exercise response and molecular target to improve insulin sensitivity. To determine if anaerobic component response, which exaggerated when sprint performed in severe acute hypoxia, influences exercise-elicited Thr(172)-AMPKα phosphorylation, 10 volunteers single 30-s (Wingate test) normoxia hypoxia (inspired Po(2): 75 mmHg). Vastus lateralis muscle biopsies were obtained before immediately after 30 120 min postsprint. Mean power...
Human skeletal muscle expresses leptin receptor mRNA; however, it remains unknown whether receptors (OB-R) are also expressed at the protein level. Fourteen healthy men (age = 33.1 ± 2.0 yr, height 175.9 1.7 cm, body mass 81.2 3.8 kg, fat 22.5 1.9%; means SE) participated in this investigation. The expression of OB-R was determined muscle, subcutaneous adipose tissue, and hypothalamus using a polyclonal rabbit anti-human receptor. Three bands with molecular close to 170, 128, 98 kDa were...
The extremely high energy demand elicited by sprint exercise is satisfied an increase in O 2 consumption combined with a glycolytic rate, leading to marked lactate accumulation, increased AMP-to-ATP ratio, and reduced NAD + /NADH.H muscle pH, which are accompanied Thr 172 AMP-activated protein kinase (AMPK)-α phosphorylation during the recovery period mechanism not fully understood. To determine role played reactive nitrogen oxygen species (RNOS) on -AMPKα response cycling exercise, nine...
Rapid oestrogen neuroprotection against beta-amyloid peptide (Abeta)-induced toxicity, a main feature of Alzheimer's disease, may be partially initiated at the plasma membrane. However, mechanism by which this effect occurs is unknown. In septal murine cell line (SN56), we observed that short exposures to either 17beta-oestradiol (E2) or membrane impermeant E2 bound horseradish peroxidase (E-HRP) induced biphasic stimulation extracellular-signal regulated protein kinase (ERK1/2)...
To examine whether obesity‐associated leptin resistance could be due to down‐regulation of receptors (OB‐Rs) and/or up‐regulation suppressor cytokine signalling 3 (SOCS3) and protein tyrosine phosphatase 1B (PTP1B) in skeletal muscle, which blunt janus kinase 2‐dependent signal transducer activator transcription (STAT3) phosphorylation reduce AMP‐activated (AMPK) acetyl‐coenzyme A carboxylase (ACC) phosphorylation. Deltoid vastus lateralis muscle biopsies were obtained from 20 men: 10...
To determine if there is a gender dimorphism in the expression of leptin receptors (OB-R170, OB-R128 and OB-R98) protein suppressor cytokine signaling 3 (SOCS3) human skeletal muscle, OB-R, perilipin A, SOCS3 alpha-tubulin was assessed by Western blot muscle biopsies obtained from m. vastus lateralis thirty-four men (age = 27.1±6.8 yr) thirty-three women 26.7±6.7 yr). Basal serum insulin concentration HOMA were similar both genders. Serum 3.4 times higher compared to (P<0.05) this difference...
To determine if muscle biopsies can be repeated using a single small (5-6 mm) skin incision without inducing immediate MAPK activation or inflammation in the noninjured areas, phosphorylation of ERK1/2, p38-MAPK, c-Jun NH(2)-terminal kinases (JNKs), IκBα, IKKα, and signal transducer activator transcription 3 (STAT3) was examined concurrent with IL-6 mRNA six obtained from vastus lateralis five men. Four were through same right leg (taken at 0, 30, 123, 126 min) another two each new incisions...
The liver responds to estrogens and GH which are critical regulators of body growth, gender-related hepatic functions, intermediate metabolism. effects on can be direct, through the direct actions ER, or indirect, include crosstalk with endocrine, metabolic, sex-differentiated functions GH. Most previous studies have been focused influence pituitary secretion, has a great impact transcriptional regulation. However, there is strong evidence that GH-regulated endocrine metabolic in human by...
This study was designed to determine whether sprint exercise activates signaling cascades linked leptin actions in human skeletal muscle and how this pattern of activation may be interfered by glucose ingestion. Muscle biopsies were obtained 15 young healthy men response a 30-s (Wingate test) randomly distributed into two groups: the fasting ( n = 7, C) group 8, G), who ingested 75 g 1 h before Wingate test. Exercise elicited different patterns JAK2, STAT3, STAT5, ERK1/2, p38 MAPK...
Hepatocellular carcinoma (HCC) is one of the most prevalent and lethal cancers worldwide, but precise intracellular mechanisms underlying progression this inflammation associated cancer are not well established. SOCS2 protein plays an important role in carcinogenesis different tumors by regulating cytokine signalling through JAK/STAT axis. However, its HCC unclear. Here, we investigate potential as biomarker. The effects were evaluated experimental model diethylnitrosamine (DEN)-induced...
Abstract Although oestrogen [17β‐estradiol (E2)]‐related neuroprotection has been demonstrated in different models, the involvement of non‐classical receptors (ERs) remains unexplored. Using SN56 cholinergic cell line, we present evidence indicating that an ER associated with plasma membrane participates oestrogen‐dependent inhibition death induced by amyloid‐β peptide (Aβ) toxicity. Similarly to E2 alone, a 15‐min exposure estradiol‐horseradish peroxidase (E‐HRP) significantly reduced...
Lung type 2 pneumocytes (T2Ps) and alveolar macrophages (AMs) play crucial roles in the synthesis, recycling catabolism of surfactant material, a lipid/protein fluid essential for respiratory function. The liver X receptors (LXR), LXRα LXRβ, are transcription factors important lipid metabolism inflammation. While LXR activation exerts anti-inflammatory actions lung injury caused by lipopolysaccharide (LPS) other inflammatory stimuli, full extent endogenous transcriptional activity pulmonary...
Recent studies have demonstrated that statins reduce cell viability and induce apoptosis in various types of cancer cells. The molecular mechanisms underlying these effects are poorly understood. JAK/STAT pathway plays an important role the regulation proliferation many tissues, its deregulation is believed to be involved tumorigenesis cancer. physiological activation STAT proteins by GH rapid but transient nature inactivation regulated mainly expression SOCS proteins. UMR-106 osteosarcoma...
The effects of basketball on basal concentrations testosterone and cortisol its associations to body composition physical performance remain be determined.The main aim this study was determine the playing position fitness, percentage fat hormonal profile in professional players (BP).Jump (SJ, CMJ ABK), 30 m running speed treadmill VO2max tests were conducted 12 male BP (24.1 years) from first division league Spain (ACB). determined anthropometry, hemoglobin, glucose, measured fasting blood...