A5037379788- Ion Channels and Receptors
- Ion channel regulation and function
- Ion Transport and Channel Regulation
- Extracellular vesicles in disease
- Magnesium in Health and Disease
- Plant Stress Responses and Tolerance
- Bioactive Compounds and Antitumor Agents
- Heavy Metal Exposure and Toxicity
- MicroRNA in disease regulation
- Hepatocellular Carcinoma Treatment and Prognosis
- Circular RNAs in diseases
- Mercury impact and mitigation studies
- Magnetic and Electromagnetic Effects
- Photoreceptor and optogenetics research
- Biochemical Analysis and Sensing Techniques
- Trace Elements in Health
- Medicinal Plant Research
- Kidney Stones and Urolithiasis Treatments
- Mobile Health and mHealth Applications
- Telemedicine and Telehealth Implementation
- Spectroscopy and Quantum Chemical Studies
- Erythrocyte Function and Pathophysiology
- Advanced Chemical Sensor Technologies
- Herbal Medicine Research Studies
- Electrolyte and hormonal disorders
University of Bucharest
1999-2024
Institutul Clinic Fundeni
2014
University of Antwerp
2011
International Centre of Biodynamics
2009
KU Leuven
2002-2005
Abstract Background The expression of microRNAs (miRNAs) is a promising prognostic and diagnostic tool in hepatocellular carcinoma (HCC). Here we performed small RNA sequencing (sRNA-seq) tissue, serum exosomes to investigate changes miRNA between the different sample types correlated with clinical parameters. We also gene arrays on tumor normal tissue. Results Paired revealed consistent positive correlation miR-21 indicating that could be exported from tissue circulation via exosomes. found...
The channels from the superfamily of transient receptor potential (TRP) activated by reactive oxygen species (ROS) are defined as redox channels. Those with best exposure cysteine residues and hence most sensitive to oxidative stress TRPC4, TRPC5, TRPV1, TRPV4, TRPA1, while others, such TRPM2 TRPM7 indirectly ROS. Activation ROS has different effects on tumorigenic process: some TRP may, upon activation, stimulate proliferation, apoptosis, or migration cancer cells, others inhibit these...
Background: Improving surgical outcomes in hepatocellular carcinoma (HCC) patients would greatly benefit from biomarkers. Angiogenesis and inflammation are hallmarks of HCC progression therapeutic targets. Methods: We retrospectively evaluated preoperative clinical variables circulating (plasma) biomarkers angiogenesis a cohort who underwent liver resection (LR) or transplantation (LT). Biomarker correlation with outcomes—freedom recurrence (FLR), disease-free survival (DFS) overall (OS)—was...
Abstract The transient receptor potential ankyrin type 1 (TRPA1) channel belongs to the TRP superfamily of ion channels. TRPA1 is a membrane protein with multiple functions able respond noxious stimuli, reactive oxygen species, inflammatory cytokines or pungent substances, and it participates in pain signalling, taste, inflammation various steps tumorigenic process. To date, no reports have addressed expression function pancreatic ductal adenocarcinoma (PDAC) cells. This work endogenous...
Recently, the transient receptor potential melastatin 8 (TRPM8) channel has emerged as a putative biomarker for pancreatic ductal adenocarcinoma (PDA). This study aimed to evaluate expression of TRPM8 and its modulation by specific agonists antagonists in PDA cells.We examined protein 3 different cell lines compared it with nontumoral epithelial line human origin using Western blotting immunocytochemical analysis. To assess function channels, we measured currents whole-cell mode patch clamp...
Pancreatic adenocarcinoma (PDAC) has low survival rates worldwide due to its tendency be detected late and characteristic desmoplastic reaction, which slows the use of targeted therapies. As such, discovery new connections between genes clinicopathological parameters contribute search for biomarkers or targets therapy. Transient receptor potential (TRP) channels are promising tools cancer therapy markers PDAC. Therefore, in this study, we selected several encoding TRP proteins previously...
We used oocytes of the South African clawed toad Xenopus laevis to express three subunits epithelial Na + channel from rat distal colon (rENaC). combined conventional dual‐microelectrode voltage‐clamp with continuous capacitance ( C m ) measurements and noise analysis evaluate effects cAMP Ni 2+ on rENaC. Control or rENaC‐expressing exhibited no spontaneous fluctuations in current. However, amiloride induced a marked plateau‐shaped rise power density spectra. Recordings using four different...
Transient receptor potential melastatin 8 (TRPM8), a membrane ion channel, is activated by thermal and chemical stimuli. In pancreatic ductal adenocarcinoma, TRPM8 required for cell migration, proliferation, senescence associated with tumor size adenocarcinoma stages. Although the underlying mechanisms of these processes have yet to be described, this cation-permeable channel has been proposed as an oncological target. study, glycosylation status was shown affect calcium uptake. expressed in...
Background: Transient receptor potential channels (TRP) are overexpressed in some pancreatic adenocarcinoma (PDAC) patients and cell lines, settling them as putative therapeutic targets this disease. Reactive oxygen species (ROS), with levels increased PDAC, modulate members of the TRP family renamed “redox channels”. Here, we investigate direct effects 4-hydroxinonenal (4-HNE) on TRPA1, natively expressed PDAC lines association migration cycle progression. Methods: We performed...
Background: The expression of the transient receptor potential 1 (TRPA1) gene is increased in many solid tumours, and its function relates to inflammation, oxidative stress or presence toxic substances. However, little known about correlation clinical parameters with patients’ cancer stages, metastases degree tumour infiltration by immune cells. Methods: We performed a bioinformatic analysis, using databases public resources investigate TRPA1 for available samples. used samples from TCGA...
The present study was conducted to investigate the renal tubular handling of inorganic cadmium (Cd(2+)) by exposing primary human cell cultures physiologically relevant doses chloride (CdCl(2)). Furthermore, cellular accumulation Cd(2+) compared that metallothionein-1-bound Cd (Cd7MT-1). Finally, this aimed effect (both and Cd7MT-1) in cells on expression genes nephrotoxic processes.Cd concentration measured using atomic absorption spectrometry. mRNA evaluated quantitative real-time...
THz spectroscopy is important for the study of ion channels because it directly addresses low frequency collective motions relevant their function. Here we used to investigate inhibition epithelial sodium channel (ENaC) by its specific blocker, amiloride. Experiments were performed on A6 cells' suspensions, which are cells overexpressing ENaC derived from Xenopus laevis kidney. spectra investigated with or without When was inhibited amiloride, a substantial increase in absorption noticed....
Opposite effects of Ni 2+ on Xenopus and rat ENaCs expressed in oocytes. Am J Physiol Cell 289: C946–C958, 2005. First published June 8, 2005; .—The epithelial Na + channel (ENaC) is modulated by various extracellular factors, including , organic or inorganic cations, serine proteases. To identify the effect divalent cation ENaCs, we compared permeability amiloride kinetics (xENaCs) (rENaCs) heterologously We found that cloned from kidney clawed toad laevis [wild-type (WT) xENaC] was...
The function of the epithelial Na+ channel from apical membrane many transporting epithelia is modulated by various chemical compounds extracellular space, such as heavy metals, protons or chloride ions. We have studied effect Cd2+ on (ENaC) in heterologously expressed Xenopus laevis oocytes and Na+-transporting epithelia. assayed amiloride-sensitive sodium current (I(Na)). rapidly voltage-independently inhibited INa expressing αβγ ENaC (xENaC). transport showed no influence trafficking,...
Background: Digital medicine (eHealth) represents using information and communications technologies to support health health-related fields with affordable secure healthcare services, medical literature, education, research. Methods: A literature search was conducted on Publons, the PubMed database, dedicated websites starting in 1995. We included papers performed different countries, specific statistical methods standardized questionnaires quantify patients' clinicians' opinions correctly....
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Identification of biomarkers that correlate with prognosis is crucial in hepatocellular carcinoma (HCC) because the high recurrence rates seen after surgical treatments.Serum levels α-fetoprotein (AFP) are widely used clinically, but prognostic value AFP remains unclear.AFP influenced not only by presence HCC also severity underlying liver disease.Furthermore, could be normal up to 30% patients.Thus, additional needed.Insulin-like growth factors 1 (IGF-1) a factor dose-dependent...