A5067516276- Endoplasmic Reticulum Stress and Disease
- Cellular transport and secretion
- Immune Cell Function and Interaction
- Bone Tissue Engineering Materials
- Glycosylation and Glycoproteins Research
- Dental materials and restorations
- Autophagy in Disease and Therapy
- Dermatology and Skin Diseases
- Exercise and Physiological Responses
- Carbohydrate Chemistry and Synthesis
- Peroxisome Proliferator-Activated Receptors
- Virus-based gene therapy research
- Ion Channels and Receptors
- Coenzyme Q10 studies and effects
- T-cell and B-cell Immunology
- Adenosine and Purinergic Signaling
- S100 Proteins and Annexins
- RNA regulation and disease
- Graphene and Nanomaterials Applications
- IL-33, ST2, and ILC Pathways
- Galectins and Cancer Biology
- Polymer Surface Interaction Studies
- Advanced Proteomics Techniques and Applications
- Whipple's Disease and Interleukins
- Genetics, Aging, and Longevity in Model Organisms
Romanian Academy
2016-2024
Institute of Biochemistry
2016-2024
Centre for Genomic Regulation
2017-2019
Barcelona Institute for Science and Technology
2019
Interleukin-21 (IL-21) is an immunostimulatory cytokine which belongs to the common gamma-chain family of cytokines. It plays import role in development, differentiation, proliferation, and activation immune cells, particular T natural killer (NK) cells. Since its discovery 2000, IL-21 has been shown regulate both adaptive responses associates with key antiviral antitumor responses. Recent advances indicate as a promising target for cancer treatment encouraging results were obtained...
An appreciation of the functional properties cytoplasmic fatty acid binding protein 4 (FABP4) has advanced with recent demonstration that an extracellular form secreted by adipocytes regulates a wide range physiological functions. Little, however, is known about mechanisms mediate unconventional secretion FABP4. Here, we demonstrate FABP4 mediated membrane-bounded compartment, independent conventional endoplasmic reticulum–Golgi secretory pathway. We show also GRASP proteins, autophagy, and...
Background: Toll-like receptors (TLRs) are a subclass of molecules that regulate both innate and adaptive immune cells, this ability makes them attractive targets for cancer immunotherapy. Synthetic TLR agonists, such as imidazoquinolines, activate TLR7/8 on various cells offer exciting perspectives therapy. Aim: In study we aimed to investigate the effects imiquimod gardiquimod, NK cell phenotype tumor growth in mouse model melanoma. Methods: C57BL/6J mice were injected subcutaneously with...
Endoplasmic reticulum (ER)-associated degradation (ERAD) is the main mechanism of targeting ER proteins for to maintain homeostasis, and perturbations ERAD lead pathological conditions. ER-degradation enhancing α-mannosidase-like (EDEM1) was proposed extract terminally misfolded from calnexin folding cycle target them by ERAD. Here, using mass-spectrometry biochemical methods, we show that EDEM1 found in auto-regulatory complexes with components. Moreover, N-terminal disordered region...
EDEM-1, EDEM-2 and EDEM-3 are key players for the quality control of newly synthesized proteins in endoplasmic reticulum (ER) by accelerating disposal degradation misfolded through ER Associated Degradation (ERAD). Although many previous studies reported role individual ERAD components especially cell-based systems, still little is known about consequences dysfunction under physiological stress conditions context a multicellular organism. Here we report first combined characterization...
Various pathologies result from disruptions to or stress of endoplasmic reticulum (ER) homeostasis, such as Parkinson's disease and most neurodegenerative illnesses, diabetes, pulmonary fibrosis, viral infections, cancers. A critical process in maintaining ER homeostasis is the selection misfolded proteins by quality-control system for destruction via ER-associated degradation (ERAD). One key protein proposed act during first steps glycoprotein degradation-enhancing α-mannosidase-like 2...
Abstract Due to their excellent mechanical and biocompatibility properties, titanium-based implants are successfully used as biomedical devices. However, when new bone formation fails for different reasons, impaired fracture healing becomes a clinical problem affects the patient's quality of life. We aimed design bioactive surface titanium with synergetic PEG biopolymer-based composition gradual delivery growth factors (FGF2, VEGF, BMP4) during healing. The optimal architecture non-cytotoxic...
Background: Transient receptor potential channels (TRP) are overexpressed in some pancreatic adenocarcinoma (PDAC) patients and cell lines, settling them as putative therapeutic targets this disease. Reactive oxygen species (ROS), with levels increased PDAC, modulate members of the TRP family renamed “redox channels”. Here, we investigate direct effects 4-hydroxinonenal (4-HNE) on TRPA1, natively expressed PDAC lines association migration cycle progression. Methods: We performed...
EDEM2 (Endoplasmic reticulum Degradation-Enhancing alpha-Mannosidase-like protein 2) is one of the key-proteins suggested to be involved in selection and degradation misfolded proteins from endoplasmic reticulum. The datasets discussed this article are related experiments covering affinity proteomics, label-free quantitative deglycoproteomics SILAC (Stable Isotope Labeling by Amino Acids Cell Culture) proteomics data A375 melanoma cells with modified expression EDEM2. Our first aim was...