A5102712942- PARP inhibition in cancer therapy
- Connexins and lens biology
- Birth, Development, and Health
- Folate and B Vitamins Research
- Cancer Diagnosis and Treatment
- Metabolomics and Mass Spectrometry Studies
- Lipoproteins and Cardiovascular Health
- Epigenetics and DNA Methylation
- Cancer Genomics and Diagnostics
- Redox biology and oxidative stress
- Erythrocyte Function and Pathophysiology
- Genetic Syndromes and Imprinting
- Immune cells in cancer
- Blood groups and transfusion
- Ginseng Biological Effects and Applications
- Hormonal Regulation and Hypertension
- Cholinesterase and Neurodegenerative Diseases
- Antioxidant Activity and Oxidative Stress
- Machine Learning in Healthcare
- Lipid metabolism and disorders
- Vitamin K Research Studies
- Sulfur Compounds in Biology
- Advanced Glycation End Products research
- Mast cells and histamine
- Optimal Experimental Design Methods
Furness General Hospital
2024
Yale University
2019-2024
Xiamen University
2024
The First People's Hospital of Shunde
2020
Southern Medical University
2020
North Sichuan Medical University
2020
Nanchong Central Hospital
2020
University of Oklahoma Health Sciences Center
2013
University of Colorado Denver
2010-2012
University of Cincinnati Medical Center
2002-2012
Glutamate-cysteine ligase (GCL) is the rate-limiting enzyme in GSH biosynthesis pathway. In higher eukaryotes, this a heterodimer comprising catalytic subunit (GCLC) and modifier (GCLM), which change characteristics of holoenzyme. To define cellular function GCLM, we disrupted mouse Gclm gene to create null allele. Gclm(−/−) mice are viable fertile have no overt phenotype. liver, lung, pancreas, erythrocytes, plasma, however, levels were 9–16% that Gclm(+/+) littermates. Cysteine inGclm(−/−)...
Organisms using oxygen for aerobic respiration require antioxidants to balance the production of reactive species during metabolic processes. Various – including humans and other primates suffer mutations in GULO gene encoding L ‐gulono‐ γ ‐lactone oxidase; is rate‐limiting enzyme biosynthesis ascorbate, an important cellular antioxidant. Animals lacking ability synthesize vitamin C develop scurvy without dietary supplementation. The Gulo( − / ) knockout (KO) mouse requires oral supplemental...
Several lines of evidence implicate lipid raft microdomains in Alzheimer disease-associated β-amyloid peptide (Aβ) production. Notably, targeting β-secretase (β-site amyloid precursor protein (APP)-cleaving enzyme 1 (BACE1)) exclusively to rafts by the addition a glycosylphosphatidylinositol (GPI) anchor its ectodomain has been reported elevate Aβ secretion. Paradoxically, secretion is not reduced expression non-raft resident S-palmitoylation-deficient BACE1 (BACE1-4C/A...
Oxidation injury to skin is one of the main reasons for aging. The aim present study was explore protective effect squid ink polysaccharides and its mechanism action against H2O2-induced dermal fibroblast damage. Our results show that effectively reduce oxidative damage mediated by up-regulation NADPH oxidase Connexin43. Concurrently, decrease ROS induced MMP1 MMP9 MMP-mediated Therefore, we hypothesize play an antioxidant role inhibiting expression connexin43. This provides a new target...
Cancer of unknown primary (CUP) represents metastatic cancer where the site remains unidentified despite standard diagnostic procedures. To determine tumor origin in such cases, we developed BPformer, a deep learning method integrating transformer model with prior knowledge biological pathways. Trained on transcriptomes from 10,410 tumors across 32 types, BPformer achieved remarkable accuracy rates 94%, 92%, and 89% sites tumors, respectively, surpassing existing methods. Additionally, was...
As one of the most abundantly expressed proteins in mammalian corneal epithelium, aldehyde dehydrogenase 3A1 (ALDH3A1) plays critical and multifaceted roles protecting cornea from oxidative stress. Recent studies have demonstrated that protective mechanism ALDH3A1 is direct absorption UV-energy, which reduces damage to other such as glucose-6-phosphate through a competition mechanism. UV-exposure, however, leads inactivation cases. In current study, we demonstrate UV-light caused soluble,...
Abstract Over the last century, outbreaks and pandemics have occurred with disturbing regularity, necessitating advance preparation large-scale, coordinated response. Here, we developed a machine learning predictive model of disease severity length hospitalization for COVID-19, which can be utilized as platform future unknown viral outbreaks. We combined untargeted metabolomics on plasma data obtained from COVID-19 patients (n = 111) during healthy controls 342), clinical comorbidity 508) to...
Andrographolide (ANDRO), a diterpenoid lactone isolated from the traditional herbal plant Andrographis paniculata, was reported to induce apoptosis in hepatoma Hep3B cells our previous study (Ji LL, Liu TY, J, Chen Y, Wang ZT. inhibits human hepatoma-derived growth through activation of c-Jun N-terminal kinase. Planta Med 2007; 73: 1397–1401). The present investigation carried out observe whether cellular reduced glutathione (GSH) plays important roles ANDRO-induced apoptosis. ANDRO...
Previously, the Evans antigen (RH37) of Rh blood group system was shown to be specified by a novel CE-D-CE hybrid gene. We studied further heterogeneity and report here its new molecular type resulting from intraexon fusion event on background D. D-- complexes.A white family with 2 Evans+ Evans- members analyzed serological methods techniques.The proband (JD) typed D+C-c-E-e- showed partial loss RHCE but an increased dose RHD DNA blots. On sequencing cDNAs, normal D 3 transcripts were...
Abstract Reliable fibre optic (FO) connection systems are a key enabling technology to deliver near real-time in-well digital data through distributed acoustic sensing (DAS), temperature (DTS) and Seismic monitoring profiling along the well. This Multi-channel Optical Feedthrough System (OFS) development addresses technical challenges provides highly reliable solution enable optical communication Vertical Monobore Tree (VMXT) Tubing Hanger (TH) while providing full pressure containment...
Abstract Despite advanced diagnostics, 3%–5% of cases remain classified as cancer unknown primary (CUP). DNA methylation, an important epigenetic feature, is essential for determining the origin metastatic tumors. We presented PathMethy, a novel Transformer model integrated with functional categories and crosstalk pathways, to accurately trace tumors in CUP samples based on methylation. PathMethy outperformed seven competing methods F1-score across nine datasets predicted molecular subtypes...
Reduced glutathione (GSH) is an abundant antioxidant that regulates intracellular redox homeostasis by scavenging reactive oxygen species (ROS). Glutamate-cysteine ligase catalytic (GCLC) subunit the rate-limiting step in GSH biosynthesis. Using