Patrick Wong
A5101463381- COVID-19 Clinical Research Studies
- SARS-CoV-2 and COVID-19 Research
- Long-Term Effects of COVID-19
- Immune Cell Function and Interaction
- SARS-CoV-2 detection and testing
- Cell Image Analysis Techniques
- Immunotherapy and Immune Responses
- Immune Response and Inflammation
- Mosquito-borne diseases and control
- Single-cell and spatial transcriptomics
- T-cell and B-cell Immunology
- Cancer Immunotherapy and Biomarkers
- RFID technology advancements
- COVID-19 diagnosis using AI
- Immune cells in cancer
- User Authentication and Security Systems
- Immunodeficiency and Autoimmune Disorders
- Machine Learning and Data Classification
- Leptospirosis research and findings
- Advanced biosensing and bioanalysis techniques
- Tryptophan and brain disorders
- HIV Research and Treatment
- Immune responses and vaccinations
- Stress Responses and Cortisol
- Gut microbiota and health
Yale University
2016-2023
Chinese University of Hong Kong
2023
Serimmune (United States)
2021
Translational Research Institute
2020
Howard Hughes Medical Institute
2016
National Institute of Allergy and Infectious Diseases
2014-2015
National Institutes of Health
2014-2015
University of Hong Kong
2010-2012
There is increasing evidence that coronavirus disease 2019 (COVID-19) produces more severe symptoms and higher mortality among men than women1–5. However, whether immune responses against acute respiratory syndrome (SARS-CoV-2) differ between sexes, such differences correlate with the sex difference in course of COVID-19, currently unknown. Here we examined viral loads, SARS-CoV-2-specific antibody titres, plasma cytokines blood-cell phenotyping patients moderate COVID-19 who had not...
Abstract Rapid and accurate SARS-CoV-2 diagnostic testing is essential for controlling the ongoing COVID-19 pandemic. The current gold standard diagnosis real-time RT-PCR detection of from nasopharyngeal swabs. Low sensitivity, exposure risks to healthcare workers, global shortages swabs personal protective equipment, however, necessitate validation new approaches. Saliva a promising candidate diagnostics because (1) collection minimally invasive can reliably be self-administered (2) saliva...
In the temperate regions, seasonal influenza virus outbreaks correlate closely with decreases in humidity. While low ambient humidity is known to enhance viral transmission, its impact on host response infection and disease outcome remains unclear. Here, we showed that housing Mx1 congenic mice relative makes more susceptible severe following respiratory challenge A virus. We find inhalation of dry air impairs mucociliary clearance, innate antiviral defense, tissue repair. Moreover,...
Abstract Dysregulated immune responses against the SARS-CoV-2 virus are instrumental in severe COVID-19. However, signatures associated with immunopathology poorly understood. Here we use multi-omics single-cell analysis to probe dynamic hospitalized patients stable or progressive course of COVID-19, explore V(D)J repertoires, and assess cellular effects tocilizumab. Coordinated profiling gene expression cell lineage protein markers shows that S100A hi /HLA-DR lo classical monocytes...
Abstract The recent spread of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) exemplifies the critical need for accurate and rapid diagnostic assays to prompt clinical public health interventions. Currently, several quantitative reverse-transcription polymerase chain reaction (qRT-PCR) are being used by clinical, research, laboratories. However, it is currently unclear if results from different tests comparable. Our goal was evaluate primer-probe sets in four common available on...
Antibodies secreted at the mucosal surface play an integral role in immune defense by serving to neutralize pathogen and promote its elimination site of entry. Secretory immunoglobulin A (IgA) is a predominant Ig isotype surfaces whose epithelial cells express polymeric receptor capable transporting dimeric IgA lumen. Although intestinal mucosa has been extensively studied, cell types responsible for secreting that protects host against pathogens lower respiratory tract are less clear. Here,...
While several clinical and immunological parameters correlate with disease severity mortality in SARS-CoV-2 infection, work remains identifying unifying correlates of coronavirus 2019 (COVID-19) that can be used to guide practice. Here, we examine saliva nasopharyngeal (NP) viral load over time them patient demographics, cellular immune profiling. We found was significantly higher those COVID-19 risk factors; it correlated increasing levels showed a superior ability as predictor (AUC=0.90)....
ABSTRACT Broadly neutralizing antibodies (bNAbs) have been isolated from selected HIV-1-infected individuals and shown to bind conserved sites on the envelope glycoprotein (Env). However, circulating plasma virus in these donors is usually resistant autologous bNAbs, indicating that during chronic infection, HIV-1 can escape even broadly cross-reactive antibodies. Here, we evaluate if such viral associated with an impairment of replication. Antibodies VRC01 class target functionally CD4...
COVID-19 manifests with a wide spectrum of clinical phenotypes that are characterized by exaggerated and misdirected host immune responses 1–8 . While pathological innate activation is well documented in severe disease 1 , the impact autoantibodies on progression less defined. Here, we used high-throughput autoantibody discovery technique called Rapid Extracellular Antigen Profiling (REAP) to screen cohort 194 SARS-CoV-2 infected patients healthcare workers for against 2,770 extracellular...
T follicular helper cells associate with development of highly mutated neutralizing antibodies against SHIV.
Compared to females, male COVID-19 patients have more kynurenic acid, which may underlie their poorer immune response.
Abstract A growing body of evidence indicates sex differences in the clinical outcomes coronavirus disease 2019 (COVID-19) 1-4 . However, whether immune responses against SARS-CoV-2 differ between sexes, and such explain male susceptibility to COVID-19, is currently unknown. In this study, we examined viral loads, SARS-CoV-2-specific antibody titers, plasma cytokines, as well blood cell phenotyping COVID-19 patients. By focusing our analysis on patients with mild moderate who had not...
T cell-B cell interaction is the key immune response to protect host from severe viral infection. However, how cells support B exert protective humoral immunity in humans not well understood. Here, we use COVID-19 as a model of acute infections and analyze CD4
Summary Recent studies have provided insights into innate and adaptive immune dynamics in coronavirus disease 2019 (COVID-19). Yet, the exact feature of antibody responses that governs COVID-19 outcomes remain unclear. Here, we analysed humoral 209 asymptomatic, mild, moderate severe patients over time to probe nature severity mortality. We observed a correlation between anti-Spike (S) IgG levels, length hospitalization clinical parameters associated with worse progression. While high anti-S...
An indirect immunoperoxidase test was compared with an fluorescent antibody and the Weil-Felix OXK for serodiagnosis of scrub typhus by measuring rickettsial antigen specific activity IgG, IgM, whole globulin. Acute convalescent sera from 50 Rickettsia tsutsugamushi isolate-positive patients 45 febrile diagnosed as having diseases other than were tested. The receiver operating characteristic each showed that tests more sensitive using acute well paired sera. no cross-reactivity when R....
Strong antibody response is considered a hallmark of successful vaccine. While dendritic cells (DCs) are important for T follicular helper (Tfh) cell priming, how this process regulated in vivo unclear. We show here that the depletion CD301b+ DCs specifically enhanced development Tfh cells, germinal center B and responses against protein antigens. Exaggerated mice depleted occurred absence any adjuvants, resulting antibodies had broader specificity higher affinity to immunogen. express high...
Abstract A dysregulated immune response against the SARS-CoV-2 virus plays a critical role in severe COVID-19. However, molecular and cellular mechanisms by which causes lethal immunopathology are poorly understood. Here, we utilize multiomics single-cell analysis to probe dynamic responses patients with stable or progressive manifestations of COVID-19, assess effects tocilizumab, an anti-IL-6 receptor monoclonal antibody. Coordinated profiling gene expression cell lineage protein markers...