A5022353700- Pain Mechanisms and Treatments
- Parasites and Host Interactions
- Neuropeptides and Animal Physiology
- Olfactory and Sensory Function Studies
- Ion Channels and Receptors
- IL-33, ST2, and ILC Pathways
- Migraine and Headache Studies
- Neurobiology and Insect Physiology Research
- Eosinophilic Esophagitis
- Parasite Biology and Host Interactions
- Pancreatic and Hepatic Oncology Research
- Biochemical effects in animals
- Diverticular Disease and Complications
- Pancreatitis Pathology and Treatment
- Circadian rhythm and melatonin
- Cancer, Stress, Anesthesia, and Immune Response
- Helicobacter pylori-related gastroenterology studies
- Antimicrobial Peptides and Activities
- Colorectal Cancer Surgical Treatments
- Genetics, Aging, and Longevity in Model Organisms
- Anesthesia and Neurotoxicity Research
- Musculoskeletal pain and rehabilitation
- Pediatric Hepatobiliary Diseases and Treatments
- Invertebrate Immune Response Mechanisms
- Neuroendocrine regulation and behavior
University of Pennsylvania
2015-2025
California University of Pennsylvania
2025
Columbia University
2021-2023
Brain (Germany)
2022-2023
Children's Hospital of Philadelphia
2015-2019
University of Iowa
2012-2019
University of Minnesota Medical Center
2015
Neurology, Inc
2015
University of Florida
2005-2012
University of Minnesota
2006-2008
Orofacial pain has been well-characterized clinically, but evaluation of orofacial in animals not kept pace. The objective this study was to describe behavioral responses facial thermal stimulation and inflammation with/without an analgesic using a novel operant paradigm. Animals were trained voluntarily place their face against stimulus thermode (37.7-57.2 degrees C) providing access positive reinforcement. These contingencies present conflict between reward tolerance for nociceptive...
Abstract Immunity against skin-invasive pathogens requires mechanisms that rapidly detect, repel or immobilize the infectious agent. While bacteria often cause painful cutaneous reactions, host skin invasion by human parasitic helminth Schistosoma mansoni goes unnoticed. This study investigated role of pain-sensing afferents express ion channel Transient Receptor Potential Vanilloid 1 (TRPV1) in detection and initiation immunity S. . Data show mice infected with have reduced behavioral...
Interleukin 33 (IL-33) is a pleiotropic cytokine released from diverse cell types that regulate both pro- and anti-inflammatory responses during pathogen infection. However, it remains unclear whether IL-33 controls key aspects of cutaneous immunity against skin-penetrating parasites. In this study, mice percutaneously infected with the parasitic helminth Strongyloides ratti were investigated to understand mechanisms anamnestic at skin barrier. Surprisingly, lacking Type 2 transcription...
Abstract Injury of the tooth pulp is excruciatingly painful and yet receptors neural circuit mechanisms that transmit this form pain remain poorly defined in both clinic preclinical rodent models. Easily quantifiable behavioral assessment mouse orofacial area remains a major bottleneck uncovering molecular govern inflammatory tooth. In study we sought to address problem using Mouse Grimace Scale novel approach application mechanical Von Frey hair stimuli. We use dental injury model exposes...
Laparoscopic adrenalectomy has been recommended as the standard method for removing an aldosteronoma. To assess our surgical experience with primary hyperaldosteronism in era of laparoscopic a 6-year retrospective review 30 consecutive patients was done. The 20 men and 10 women ranged age from 35 to 78 mean 51.2 years. All were hypertensive hypokalemic serum potassium 2.9 +/- 0.32 (standard deviation) mmol/L. Serum aldosterone elevated 28 (94%) normal remaining two. renin suppressed all...
Background: Rodent models of orofacial pain typically use methods adapted from manipulations to hind paw; however, limitations these include animal restraint and subjective assessments behavior by the experimenter. In contrast methods, assessment operant responses painful stimuli has been shown overcome expand breadth interpretation behavioral responses. current study, we used an model based on a reward-conflict paradigm assess nociceptive in three strains mice (SKH1-Hr hr , C57BL/6J, TRPV1...
A hallmark of many orofacial pain disorders is cold sensitivity, but relative to heat-related pain, mechanisms perception and the development allodynia are not clearly understood. Molecular mediators sensation such as TRPM8 have been recently identified characterized using in vitro studies. In this study we operant behavior with respect individually presented stimuli (24, 10, 2, -4 degrees C) a thermal preference task where rats chose between 48 C stimulation. We also evaluated effects...
Abstract Background Obesity is a risk factor associated with several pain syndromes. However, the mechanisms underlying association between obesity and are not known. The aim of this study was to test hypothesis that enhances neuronal responses nociceptive stimulation within trigeminal nucleus caudalis ( TNC ). Methods Male female C57BL/6J mice were fed high‐fat or regular diet from time weaning until 20 weeks age. We then quantified activation by measuring F os immunoreactivity in response...
The impact of the host immune environment on parasite transcription and fitness is currently unknown. It widely held that hookworm infections have an immunomodulatory host, but whether converse true remains unclear. Immunity against adult-stage hookworms largely mediated by Type 2 responses driven factor Signal Transducer Activator Transcription 6 (STAT6). This study investigated serial passage rodent Nippostrongylus brasiliensis in STAT6-deficient mice (STAT6 KO) caused changes parasites...
Co-evolutionary adaptation of hookworms with their mammalian hosts has been selected for immunoregulatory excretory/secretory (E/S) products. However, it is not known whether, or if so, how host immunological status impacts the secreted profile hematophagous adult worms. This study interrogated impact Signal transducer and activator transcription 6 (STAT6) expression during experimental evolution through sequential passage life cycle in either STAT6 deficient WT C57BL/6 mice. Proteomic...
In Brief Obesity is associated with several pain disorders including headache. The effects of obesity on the trigeminal nociceptive system, which mediates headache, remain unknown. We used 2 complementary mouse models (high-fat diet and leptin deficiency) to examine this. assessed capsaicin-induced nocifensive behavior photophobia in obese control mice. Calcium imaging was determine activity primary afferents vitro. found that mice had a normal acute response facial injection capsaicin, but...
Management of pain involves a balance between inhibition and minimization side effects; therefore, in developing new analgesic compounds, one must consider the effects treatment on both processing behavior. The purpose this study was to evaluate mu kappa-2 opioid receptor agonists general behavioral outcomes. As assessment, we modified cylinder rearing assay recorded number duration events. Thermal sensitivity evaluated using either reflexive measure hindpaw withdrawal latency radiant heat...
A prospective assessment was performed to determine the incidence of anal complications after ileoanal J-pouch anastomosis procedures for ulcerative colitis (UC) and familial adenomatous polyposis (FAP). From 1989 2000, 75 patients (50 male 25 female) underwent proctocolectomy ileal pouch-anal with temporary loop ileostomy UC (N = 68) FAP 7). Overall 33 (44%) developed postoperatively. Nineteen (25%) had mild stenosis amenable digital dilatation in office. Ten (13%) severe requiring...
The gastrointestinal (GI) nematode Strongyloides stercoralis (S.s.) causes human strongyloidiasis, a potentially life-threatening disease that currently affects over 600 million people globally. uniquely pernicious aspect of S.s. infection, as compared to all other GI nematodes, is its autoinfective larval stage (L3a) maintains low-grade chronic allowing undetectable persistence for decades. Infected individuals who are administered glucocorticoid therapy can develop rapid and often lethal...