A5025750577- CAR-T cell therapy research
- Acute Lymphoblastic Leukemia research
- Immunotherapy and Immune Responses
- Immune Cell Function and Interaction
- Acute Myeloid Leukemia Research
- Chronic Lymphocytic Leukemia Research
- Single-cell and spatial transcriptomics
- Nanowire Synthesis and Applications
- T-cell and B-cell Immunology
- Chronic Myeloid Leukemia Treatments
- Allergic Rhinitis and Sensitization
- RNA modifications and cancer
- Sinusitis and nasal conditions
- SARS-CoV-2 and COVID-19 Research
- Lymphoma Diagnosis and Treatment
- Virus-based gene therapy research
- Cancer-related molecular mechanisms research
- Hematopoietic Stem Cell Transplantation
- RNA Research and Splicing
- Infectious Diseases and Mycology
Children's Hospital of Los Angeles
2015-2025
The University of Texas Health Science Center at Houston
2024
University of Southern California
2021
Children's Center
2015-2017
Abstract Chimeric antigen receptor (CAR) T-cells targeting CD19 demonstrate remarkable efficacy in treating B-lineage acute lymphoblastic leukemia (BL-ALL), yet up to 39% of treated patients relapse with CD19(−) disease. We report that escape is associated downregulation, but preservation, targetable expression CD20 and CD22. Accordingly, we reasoned broadening the spectrum CD19CAR include both CD22 would enable them target BL-ALL while preserving their upfront efficacy. created a...
Severe chronic rhinosinusitis with nasal polyps (CRSwNP), a form of diffuse bilateral (usually type 2) CRS, is debilitating disease significant impact on quality life (QoL). With novel knowledge and treatment options becoming available, there growing need to update or revise key definitions enable communication across different specialties dealing agree goals care in CRSwNP. The European Forum for Research Education Allergy Airway diseases (EUFOREA) EPOS expert members discussed how measure...
Abstract Little is known about the efficacy of COVID‐19 vaccines during acute lymphoblastic leukemia therapy (ALL); data for vaccine immune responses in pediatric remain sparse. We conducted a single center study patients aged 5–25 years undergoing ALL chemotherapy who received vaccination. Twenty‐one were enrolled; was evaluable 20. Twenty vaccinated while receiving chemotherapy. BNT162b2 mRNA vaccine. Spike reactive antibodies (S‐IgG) and/or T‐cells (SRT) detected 16 20 (80%) patients; 13...
Single cell RNA sequencing of human thymic cells is dependent on isolation highly pure and viable populations. This protocol describes the CD34+ progenitor more differentiated CD34- fractions from post-natal tissue to study thymopoiesis. represent <1% cells, so this uses magnetic- followed by fluorescence-activated separation isolate enriched cells. For complete details use execution protocol, please refer Le et al. (2020).
The bone marrow is a frequent location of primary relapse after conventional cytotoxic drug treatment human B-cell precursor acute lymphoblastic leukemia (BCP-ALL). Because stromal cells have major role in promoting chemotherapy resistance, they should be included to more realistically model vitro treatment. Here we validated novel application the xCELLigence system as continuous co-culture assess long-term effects on BCP-ALL cells. We found that OP9 adhere electrodes but are progressively...