Abstract Aims and Objectives The purpose of this study was to understand the emotional intelligence level (EI) negative status front‐line nurses in epidemic situation further explore relationship between them. Background During COVID‐19 epidemic, under influence multiple factors, were vulnerable emotions. While previous studies have explored, role emotions, two has not been sufficiently discussed context COVID‐19. Design carried out a cross‐sectional survey. STROBE selected as checklist...

Photosynthesis can be impaired by combined high light and temperature (HLHT) stress. Obtaining HLHT tolerant photoautotrophs is laborious time-consuming, in most cases the underlying molecular mechanisms remain unclear. Here, we increase mutation rates of cyanobacterium Synechococcus elongatus PCC 7942 three orders magnitude through combinatory perturbations genetic fidelity machinery cultivation environment. Utilizing hypermutation system, isolate mutants with improved tolerance identify...

Mannitol is a valuable sugar alcohol, extensively used across various industries. Cyanobacteria show potential as future platforms for mannitol production, utilizing CO 2 and solar energy directly. The proof-of-concept has been demonstrated by introducing two-step pathway in cyanobacteria, converting fructose-6-phosphate to mannitol-1-phosphate sequentially mannitol. However, recombinant strains generally faced issues of genetic instability or low titers, consequently affecting the long-term...

In the present study, an oligosaccharide SPO-1 from Spirulina platensis was prepared by glycosidase a marine bacterium. The prebiotic activity of on growth Lactobacillus paracasei and Bifidobacterium animalis, its effect human gut microbiota were examined in vitro. molecular weight tetrasaccharide 650.2 Da, it mainly composed glucose with α-type glycosidic linkages. score highest for probiotic strains L. B. animalis. Furthermore, as fermentation proceeded, gradually degraded utilized...

Cyanobacteria are supposed to be promising photosynthetic microbial platforms recycling carbon dioxide driven by solar energy into biomass and bioproducts. Glycogen synthesis serves as an essential natural sink mechanism, storing a large portion of organic source from photosynthesis. Engineering glycogen metabolism harness rewire flow is important strategy optimize efficacy cyanobacteria platforms. ADP-glucose pyrophosphorylase (GlgC) catalyzes the rate-limiting step for synthesis. However...

Fructose is an important monosaccharide product widely applied in the food, medicine, and chemical industries. Currently, fructose mainly manufactured with plant biomass-sourced polysaccharides through multiple steps of digestion, conversion, separation, purification. The development cyanobacterial metabolic engineering provides attractive alternative route for one-step direct production utilizing carbon dioxide solar energy. In this work, we developed a paradigm chassis cells into efficient...

Chemotherapy is generally accompanied by undesirable side effects, such as immunosuppression and malnutrition, which reduce tolerance to cancer therapies. Prior studies have shown that immunonutrition improves the clinical outcomes of patients. In this study, immunoregulatory polysaccharides from Crassostrea hongkongensis were included in a nutrition formula was administered S180 tumor-bearing mice combination with 5-fluorouracil (5-FU) treatment. The C30-60% fraction characterized branched...

Chemotherapeutics, including 5-fluorouracil (5-FU), often damage the intestinal mucosal barrier and cause mucositis (IM). Supplementation with immunoregulatory polysaccharides from Crassostrea hongkongensis has been shown to positively influence effectiveness toxicity of 5-FU. Therefore, we studied effects oyster on 5-FU-induced in rats. The C30–60% ethanol-precipitated fraction promoted IEC-6 cell proliferation exhibited a maximal effect at 0.0195 mg/mL concentration. Moreover, treatment...

In the present work, analysis of hypolipidemic properties Trachinotus ovatus protein hydrolysates (TOPHs) and identification peptides with bile acid-binding activity were performed. Hydrolysates prepared by trypsin digestion exhibited highest in vitro capacities compared other four proteases mainly composed small amino acids molecular weights <3 kDa, accounting for 77.30%. Among five ultra-filtration fractions TOPHs, TOPHs-5 (<3 kDa) capacity, which was equivalent to 77.97% cholestyramine at...

Abstract Valencene is a natural sesquiterpene with desirable bioactivity and aroma, making it valuable ingredient in the food cosmetics industries. Traditionally, valencene was extracted from citrus fruits, its applications were restricted by low concentrations sources high costs for extraction. Photosynthetic biomanufacturing represents promising route efficient stable production of valencene, while cyanobacteria have been considered one most platforms regarding biotechnological routes...

In this study, Tuna trimmings (Thunnas albacares) protein hydrolysate (TPA) was produced by alcalase. The anti-tumor synergistic effect and intestinal mucosa protective of TPA on S180 tumor-bearing mice treated with 5-fluorouracil (5-FU) chemotherapy were investigated. results showed that can enhance the 5-FU chemotherapy, as evident a significant reduction in tumor volume observed medium high dose TPA+5-FU groups compared to group (p < 0.001). Moreover, significantly elevated content total...

The beneficial effects of oyster extract against various disorders and diseases induced by oxidative stress have aroused great interest. In this article, ultrasonic-assisted enzymolysis was employed to produce polysaccharides Crassostrea hongkongensis (CHP) their antioxidant activity investigated. A single-factor experiment then a four-factor, three-level Box-Behnken design were first used optimize ultrasonic extraction for polysaccharides. On the basis ridge analysis, optimum conditions are...

The traditional Chinese medicine theory believes that octopus papaya soup can stimulate milk production in lactating women. objective of this study was to determine whether dietary supplementation with an enzymatic hydrolysate Octopus vulgaris and Carica (EHOC) could increase nutritional indexes Sprague Dawley (SD) rats. Female SD rats (n = 24) were fed a control diet 8), EHOC-supplemented diet, or positive (Shengruzhi) from day 10 pregnancy lactation. Maternal serum, mammary gland (day...

Cyanobacteria are important model organisms for exploring the mechanisms of photosynthesis and considered as promising microbial platforms photosynthetic biomanufacturing. The development efficient cyanobacteria cell factories requires convenient tools to dynamically regulate manipulate target proteins, modules, pathways. Targeted protein degradation is achieve rapid responses cellular metabolic networks artificial or environmental signals, there currently limited approaches induce in...