Abstract An outbreak of coronavirus disease 2019 (COVID‐19) occurred in Wuhan and it has rapidly spread to almost all parts the world. For coronaviruses, RNA‐dependent RNA polymerase (RdRp) is an important that catalyzes replication from template attractive therapeutic target. In this study, we screened these chemical structures traditional Chinese medicinal compounds proven show antiviral activity severe acute respiratory syndrome (SARS‐CoV) similar through a molecular docking study target...

4-Coumarate:CoA ligases (4CLs, EC 6.2.1.12 ) are a group of enzymes necessary for maintaining continuous metabolic flux the biosynthesis plant phenylpropanoids, such as lignin and flavonoids, that essential to survival plants. So far, various biochemical molecular studies 4CLs seem suggest 4CL isoforms in plants functionally indistinguishable mediating these phenolics. However, we have discovered two structurally distinct genes, Pt4CL1 Pt4CL2 (63% protein sequence identity), differentially...

Autophagy is a programmed cell survival mechanism that has key role in both physiologic and pathologic conditions. The relationship between autophagy cancer complex because can act as either tumour suppressor or promoter. of oral squamous carcinoma (OSCC) controversial. Several studies have claimed high low expression autophagy-related proteins was associated with poor prognosis OSCCs. aims the study were to compare OSCCs, verrucous hyperplasias, normal mucosas, inspect prognostic OSCCs.We...

Epidermal growth factor receptor (EGFR) activation has been demonstrated to have a critical role in tumor angiogenesis. In the present study, correlation between EGFR mutations and vascular endothelial (VEGF) was investigated lung cancer cell lines non-small-cell (NSCLC) tissues. VEGF levels were significantly increased culture medium of cells NSCLC tissues with (H1650 vs. A549, P=0.0399; H1975 P<0.0001). Stable expressing mutant (exon 19 deletion, E746-A750; exon 21 missense mutation,...

The benefit of cytoreduction with hyperthermic intraperitoneal chemotherapy (CRS/HIPEC) for epithelial ovarian cancer (EOC) remains uncertain. This study investigated the relationship between serum cytokines, particularly monocyte chemoattractant protein-1 (MCP-1), a key inflammatory mediator, and recurrence risk in EOC patients undergoing CRS/HIPEC. From January 2018 to 2023, cytokine levels were analyzed 34 (17 primary, 17 recurrent) before after CRS/HIPEC using MILLIPLEX Magnetic Bead...

Lynch syndrome is an autosomal dominant disorder predisposing individuals to colorectal and other cancers, primarily caused by variants in mismatch repair genes. This study describes a novel MSH6 variant affecting transcript structure Taiwanese family meeting the Amsterdam II criteria for syndrome. A 67-year-old male presented with jejunal adenocarcinoma strong history of cancer. Immunohistochemistry revealed loss expression, while next-generation sequencing performed on tumor tissue failed...

For rapid and unlimited cell growth proliferation, cancer cells require large quantities of nutrients. Many metabolic pathways nutrient uptake systems are frequently reprogrammed upregulated to meet the demand from cells, including for lipids. The lipids most adult normal mainly acquired circulatory system. Whether different adopt identical mechanisms ensure sufficient lipid supply, whether supply each other, remains unclear, was investigated in lung cells. Results showed that, despite...

Epidermal growth factor receptor (EGFR) activation has been shown to play a critical role in tumor angiogenesis. In this study, we investigate the correlation between EGFR mutations and cadherin-5 (CDH5), which is an angiogenic factor, lung cancer cells. Increased expression CDH5 observed cells with mutations. Stable cell lines expressing mutant (exon 19 deletion E746-A750, exon 21 missense mutation L858R) wild type genes are established. A significantly higher of stable mouse xenografts....

Background Beclin 1 and 2 are autophagy-related proteins that show similar amino acid sequences domain structures. established the first connection between autophagy cancer. However, role of in cancer is unclear. The aims this study were to analyze expressions oral tissues cell lines, evaluate their possible roles progression. Methods We investigated by immunohistochemistry 195 cases prognostic analyzed statistically. In vitro, overexpression knockdown performed on an line, SAS....

Regulator of G protein signaling 11 (RGS11), a member the R7 subfamily RGS proteins, is well-characterized GTPase-accelerating that involved in heterotrimeric regulation amplitude and kinetics receptor-promoted retinal bipolar nerve cells. However, role RGS11 cancer completely unclear. Using subtractive hybridization analysis, we found was highly expressed lymph-node metastatic tissues bone-metastatic tumors obtained from patients with lung adenocarcinoma. Characterization...

Cullin 4A (Cul4A) is overexpressed in a number of cancers and has been established as an oncogene. This study aimed to elucidate the role Cul4A lung cancer invasion metastasis. We observed that was non-small cell (NSCLC) tissues overexpression associated with poor prognosis after surgical resection it also decreased expression tumor suppressor protein annexin A10 (ANXA10). The knockdown upregulation ANXA10, forced downregulation ANXA10 cells. Further studies showed inhibited metastasis...

The c-myc promoter-binding protein-1 (MBP-1) is a transcriptional suppressor of tumorigenesis and thought to be the product alternative translation initiation α-enolase (ENO1) transcript. In present study, we cloned 2552-bp novel cDNA with putative coding sequence MBP-1 functionally examined its ability encode protein. Similarly ENO1, obtained was widely differentially expressed in variety normal tissues cancer cells. Experiments using promoter-driven luciferase reporter assays, biochemical...

Abstract Epithelial–mesenchymal transition (EMT) is a critical process for inducing stem-like properties of epithelial cancer cells. However, the role EMT inducers in hematological malignancies unknown. Twist1 , an inducer necessary cell migration, has recently been found to have transcriptionally regulatory activity on expression Bmi1 and these two are capable promoting tumorigenesis synergized manner. Knowing that essential maintenance leukemic stem cells, we speculate might govern...

High mobility group AT-hook 2 (HMGA2) is an architectural transcription factor that negatively regulated by let-7 microRNA through binding to it's 3′-untranslated region. Transgenic mice expressing Hmga2 with a truncation of its region has been shown exhibit myeloproliferative phenotype. To decipher the let-7-HMGA2 axis in neoplasms, we employed vitro model supplemented clinical correlation. Ba/F3 cells inducible JAK2V617F expression (Ton.JAK2.V617F cells) showed upregulation HMGA2...

Abstract Cullin 4A (Cul4A) has been observed to be overexpressed in various cancers. In this study, the role of Cul4A growth and chemosensitivity lung cancer cells were studied. We showed that is tissues. Knockdown expression by sh RNA resulted decreased cellular proliferation cells. Increased sensitivity gemcitabine, a chemotherapy drug, was also noted those knockdown Moreover, increased p21, transforming factor ( TGF )‐β inducible early gene‐1 (TIEG1) beta‐induced (TGFBI) after knockdown,...

ObjectiveEpstein–Barr virus-positive diffuse large B-cell lymphoma is a provisional entity in the 2008 World Health Organization classification of tumors hematopoietic and lymphoid tissues. Reports on characteristics clinical outcome this disease different geographic regions showed great disparities.

The ongoing COVID-19 pandemic, caused by SARS-CoV-2, continues to pose significant global health challenges. results demonstrated that GB-2 at 200 μg/mL effectively increased the population of 293T-ACE2 cells with low RBD binding for both SARS-CoV-2 Omicron EG.5.1 and HV.1 variants dual-color flow cytometry, indicating its ability inhibit virus attachment. Further investigation revealed (+)-catechin 25 50 did not significantly alter ACE2-RBD interaction variant. In contrast, theaflavin...

Unlimited growth of cancer cells requires an extensive nutrient supply. To meet this demand, drastically upregulate glucose uptake and metabolism compared to normal cells. This difference has made the blocking glycolysis a fascinating strategy treat malignant disease. α-enolase is not only one most upregulated glycolytic enzymes in cells, but also associates with many cellular processes or conditions important cell survival, such as migration, invasion, hypoxia. Targeting could...

Somatic mutations of MET gene are emerging as important driver for lung cancers. To identify the common clinicopathological features exon 14 skipping and amplification clarify whether two alterations cause protein overexpression were investigated using 196 cancer samples Taiwan through real time-qPCR/sequencing, fluorescence in situ hybridization, immunohistochemistry. The both present low frequency, ~1%, studied population Taiwan. identified from early-stage patients, who relatively...