A5101565810- Cancer-related molecular mechanisms research
- MicroRNA in disease regulation
- RNA Research and Splicing
- Circular RNAs in diseases
- RNA modifications and cancer
- PI3K/AKT/mTOR signaling in cancer
- RNA Interference and Gene Delivery
- Mesenchymal stem cell research
- Epigenetics and DNA Methylation
- interferon and immune responses
- Extracellular vesicles in disease
- Cancer, Hypoxia, and Metabolism
- Plant Parasitism and Resistance
- Cancer Research and Treatments
- NF-κB Signaling Pathways
- Aortic Disease and Treatment Approaches
- Hearing, Cochlea, Tinnitus, Genetics
- Cancer Mechanisms and Therapy
- Nanoplatforms for cancer theranostics
- Pluripotent Stem Cells Research
- Aortic aneurysm repair treatments
- Liver physiology and pathology
- Advanced Breast Cancer Therapies
- Wnt/β-catenin signaling in development and cancer
- Influenza Virus Research Studies
First Affiliated Hospital of Zhengzhou University
2020-2025
Guizhou Provincial People's Hospital
2015-2024
Zaozhuang University
2022-2023
Weifang University
2011-2021
Second Affiliated Hospital of Nanjing Medical University
2021
Sanofi (United States)
2013-2020
Central South University
2019-2020
Zhengzhou University
2020
Nanjing Brain Hospital
2019
Nanjing Medical University
2019
Abstract Background Although trastuzumab provides significant clinical benefit for HER2-positive breast cancers, responses are limited by the emergence of resistance. Recent evidence suggests that long noncoding RNAs (lncRNAs) play important roles in tumorigenesis and chemoresistance. However, regulatory mechanism lncRNAs resistance is not well established to date. In this research, we identified differentially expressed lncRNA investigated its role cancer. Methods LncRNA microarray qRT-PCR...
Trastuzumab is commonly used in the treatment of human epidermal growth factor receptor-2 positive (HER-2+) breast cancer, but its efficacy often limited by emergence chemoresistance. Recent studies indicate that exosomes act as vehicles for exchange genetic cargo between heterogeneous populations tumor cells, engendering a transmitted drug resistance cancer development and progression. However, specific contribution cancer-derived poorly understood. In this study, publicly available...
Abstract Hepatocellular carcinoma (HCC) remains a significant clinical challenge with few therapeutic options available to cancer patients. MicroRNA 21-5p (miR-21) has been shown be upregulated in HCC, but the contribution of this oncomiR maintenance tumorigenic phenotype liver poorly understood. We have developed potent and specific single-stranded oligonucleotide inhibitors miR-21 (anti-miRNAs) used them interrogate dependency on panel cell lines. Treatment anti–miR-21, not mismatch...
Primary treatment for estrogen receptor-positive (ER+) breast cancer is endocrine therapy. However, substantial evidence indicates a continued role ER signaling in tumor progression. Selective receptor degraders (SERD), such as fulvestrant, induce effective inhibition, although clinical studies with fulvestrant report insufficient blockade of signaling, possibly due to suboptimal pharmaceutical properties. Furthermore, activating mutations the have emerged resistance mechanism current...
The research aimed to examine the expression of lncRNA H19, miR‐188, and LCoR in mouse bone marrow stromal stem cells (mBMSCs), investigate regulatory mechanism H19/miR‐188/ osteogenic adipogenic differentiation mBMSCs. miR‐188 mBMSCs osteogenesis induced was detected by stem‐loop RT‐PCR, while H19 adipogenesis examined qRT‐PCR. Luciferase reporter assay verified targeted relationship between or . Cell proliferation ability determined MTT assay, cell surface markers were analyzed via flow...
Hepatocellular carcinoma (HCC) is a deadly malignancy with limited treatment options. Activation of the AKT/mTOR cascade one most frequent events along hepatocarcinogenesis. mTOR serine/threonine kinase and presents in two distinct complexes: mTORC1 mTORC2. While has been extensively studied HCC, functional contribution mTORC2 during hepatocarcinogenesis not well characterized, especially vivo. Pten expression major mechanisms leading to aberrant activation signaling. Here, we show that...
The regulatory mechanism of long non-coding RNAs (lncRNAs) in trastuzumab resistance is not well established to date. In this research, we identified differentially expressed lncRNA and investigated its role breast cancer. HiSeq sequencing quantitative real-time PCR were performed identify the dysregulated lncRNAs. Mass spectrometry, RNA fluorescence situ hybridization (RNA-FISH), immunoprecipitation assays direct interactions between ZNF649-AS1 other associated targets, such as...
Mechanisms of unassisted delivery RNA therapeutics, including inhibitors microRNAs, remain poorly understood. We observed that the hepatocellular carcinoma cell line SKHEP1 retains productive free uptake a miR-21 inhibitor (anti-miR-21). Uptake anti-miR-21, but not mismatch (MM) control, induces expression known targets (DDAH1, ANKRD46) and leads to dose-dependent inhibition growth. To elucidate mechanisms sensitivity we conducted an unbiased shRNA screen revealed tumor susceptibility gene...
Objective: To investigate the clinical characteristics, prognosis of different treatments and influencing factors hepatic myelopathy (HM). Methods: The data 134 HM patients treated in First Affiliated Hospital Zhengzhou University from June 2016 to 2023 were retrospectively analyzed. age was (53.6±9.0) years old, with 114 males 20 females. According treatment methods, they divided into drug group (n=95), intervention (n=23) liver transplantation (n=16); according types shunt, spontaneous...
Previous study has demonstrated that long noncoding RNA cyclin-dependent kinase inhibitor 2B antisense 1 (CDKN2B-AS1) was abnormally expressed in diabetic nephropathy (DN). However, the underlying mechanism allows CDKN2B-AS1 progression of DN remains to be further elucidated.Peripheral blood cells 24 diabetes patients with and 20 without were collected. Human glomerular mesangial (HGMC) cultured high glucose or low medium. The expression levels CDKN2B-AS1, microRNA (miR)-424-5p mobility...
MicroRNAs have been confirmed to be a group of important regulators during the pathogenesis nasopharyngeal carcinoma (NPC). This study that expression microRNA-92a (miR-92a) was significantly upregulated in NPC as compared noncancerous epithelial tissues. Furthermore, high miR-92a observed all cell lines, especially metastatic lines. Clinical analysis indicated associated with adverse clinicopathological features including advanced tumor-node-metastasis stage and distant metastasis,...
Trastuzumab has been widely used for treatment of HER-2-positive breast cancer patients, however, the clinical response restricted due to emergence resistance. Recent studies indicate that long noncoding RNA AGAP2-AS1 (lncRNA AGAP2-AS1) plays an important role in However, precise regulatory function and therapeutic potential trastuzumab resistance is still not defined. In this study, we sought reveal essential Our results suggest disseminates via packaging into exosomes. Exosomal induces...
Studies have shown that male germline stem cells (mGSCs), which are responsible for maintaining spermatogenesis in the male, could be obtained from mouse and human testis. However, traditional cultural methods were mostly dependent on serum feeder, initial mGSCs either neonatal mice or detailed description of its potency origin was not provided. Here we reported a novel (retinol (RE) serum-free feeder-free) system successful culture adult Kunming (8-24 weeks) The isolated cultured RE...
A small molecular chemical-Reversine has been shown to promote cell reprogramming and induce dedifferentiation of multiple terminally differentiated mesodermal origin cells, then differentiate into other types within lineages as well neuroectodermal. However, the possibilities these cells give rise germ have not examined. The objective current study was detect effect Reversine on PMDSCs differentiation cells. from fetal porcine skeletal muscle their potential in vitro were investigated....
Abstract To investigate the effect of low-intensity pulsed ultrasound (LIPUS) on proliferation human adipose-derived mesenchymal stromal cells (hASCs) and uncovered its stimulation mechanism. LIPUS at 30 mW/cm 2 was applied for 5 min/day to promote hASCs. Flow cytometry used study cell surface markers, cycle, apoptosis The hASCs detected by counting kit-8, cycle assay, RT-PCR. expression cytokines determined ELISA. differences between transcriptional genes metabolites were analyzed...
Hepatocellular carcinoma (HCC) is a leading cause of cancer related deaths worldwide. The PI3K cascade one the major signaling pathways underlying HCC development and progression. Activating mutations catalytic subunit alpha (PIK3CA) and/or loss Pten often occur in human HCCs. Serum glucocorticoid kinase 3 (SGK3) belongs to SGK family AGK kinases functions parallel AKT downstream PI3K. Previous studies have shown that SGK3 may be responsible for oncogenic potential PIK3CA helical domain...
Abstract Background Skeletal muscle development, a long-term and complex process, is controlled by set of the myogenic genes. Circular RNAs (circRNAs), class noncoding RNA, have been shown to regulate various biological processes. Recent studies indicate circRNAs may be involved in myogenesis, but role regulatory mechanism myogenesis largely unknown. In present study, circCPE was firstly found promote bovine myoblast proliferation inhibit cell apoptosis differentiation influencing expression...
Hepatocellular carcinoma (HCC) represents a serious public health challenge with few therapeutic options available to cancer patients.Wnt/β-catenin pathway is thought play significant role in HCC pathogenesis. In this study, we confirmed high frequency of CTNNB1 (β-catenin) mutations two independent cohorts patients and demonstrated upregulation β-catenin protein the overwhelming majority patient samples, patient-derived xenografts (PDX) established cell lines. Using genetic tools validated...