A5030798280- MicroRNA in disease regulation
- Nanoplatforms for cancer theranostics
- Nanoparticle-Based Drug Delivery
- Cancer Cells and Metastasis
- Tissue Engineering and Regenerative Medicine
- Cancer Research and Treatments
- Cancer, Hypoxia, and Metabolism
- Polyamine Metabolism and Applications
- Probiotics and Fermented Foods
- Pancreatic and Hepatic Oncology Research
- Molecular Biology Techniques and Applications
- Angiogenesis and VEGF in Cancer
- Protein Hydrolysis and Bioactive Peptides
- X-ray Diffraction in Crystallography
- Immune cells in cancer
- Microbial Metabolites in Food Biotechnology
- Bacteriophages and microbial interactions
- Crystallization and Solubility Studies
- Wnt/β-catenin signaling in development and cancer
- RNA Interference and Gene Delivery
- Biochemical and Structural Characterization
- Enzyme Production and Characterization
- Digestive system and related health
- PI3K/AKT/mTOR signaling in cancer
- Cancer Genomics and Diagnostics
Toyama Prefectural Agricultural, Forestry & Fisheries Research Center
2009-2023
Kanazawa University
2009-2023
The University of Texas MD Anderson Cancer Center
2004-2023
Houston Methodist
2012-2022
Food Research Institute
2011-2021
National Defense Academy of Japan
2019
Nagoya University
1996-2016
Vilnius University
2015
Methodist Hospital
2013
Fukui University of Technology
2012
Hypoxia, frequently found in the center of solid tumor, is associated with resistance to chemotherapy by activation signaling pathways that regulate cell pro-liferation, angiogenesis, and apoptosis. We determined whether hypoxia can increase human pancreatic carcinoma cells gemcitabine-induced apoptosis phosphatidylinositol 3'-kinase (PI3K)/Akt, MEK/mitogen-activated protein kinase (extracellular signal-regulated kinase) [MAPK(Erk) (MEK)], nuclear factor kappa B (NF-kappa B) pathways.We...
Abstract Porous silicon (pSi) is emerging as a promising material in the development of nanovectors for systemic delivery therapeutic and imaging agents. The integration photolithographic patterning, typical semiconductor industry, with electrochemical etching provides highly flexible strategy to fabricate monodisperse precisely tailored nanovectors. Here, microfabrication direct lithographic patterning discoidal pSi particles presented that enables precise independent control over particle...
Abstract We studied growth factors and their receptors in tumor cells tumor-associated endothelial as the therapeutic targets colon cancer. Immunohistochemical analysis of 13 surgical specimens human adenocarcinoma revealed that both 11 expressed epidermal factor (EGF), transforming α (TGF-α), EGF receptor (EGFR), phosphorylated EGFR (pEGFR), vascular (VEGF), VEGF (VEGFR), VEGFR (pVEGFR). HT29 cancer growing orthotopically cecum nude mice a high level EGF, EGFR, pEGFR, VEGF, VEGFR, pVEGFR....
Abstract Purpose: Pancreatic adenocarcinoma is currently the fourth leading cause of cancer death in United States, and most pancreatic cancers develop locally advanced disease or metastasis at time diagnosis. The mechanisms by which it invades metastasizes are not known. Experimental Design: To identify genes involved metastasis, we analyzed gene expression profiles between highly metastatic Colo357L3.6pl parental Colo357FG cell lines using cDNA microarrays confirmed differential reverse...
Glycogen synthase kinase-3beta (GSK3beta) regulates multiple cell signaling pathways and has been implicated in glucose intolerance, neurodegenerative disorders, inflammation. We investigated the expression, activity, putative pathologic role of GSK3beta gastrointestinal, pancreatic, liver cancers.Colon, stomach, cancer lines; nonneoplastic HEK293 cells; matched pairs normal tumor tissues stomach colon patients were examined for expression its phosphorylation at serine 9 (inactive form)...
The capillary wall is the chief barrier to tissue entry of therapeutic nanoparticles, thereby dictating their efficacy. Collagen fibers are an important component walls, affecting leakiness in healthy or tumor vasculature. Using a computational model along with vivo systems, we compared how collagen structure affects diffusion flux 1-nm chemotherapeutic molecule [doxorubicin (DOX)] and 80-nm chemotherapy-loaded pegylated liposome (DOX-PLD) We found direct correlation between content around...
Current treatments for liver metastases arising from primary breast and lung cancers are minimally effective. One reason this unfavorable outcome is that poorly vascularized, limiting the ability to deliver therapeutics systemic circulation lesions. Seeking enhance transport of agents into tumor microenvironment, we designed a system in which nanoparticle albumin-bound paclitaxel (nAb-PTX) loaded nanoporous solid multistage nanovector (MSV) enable passage drug through vessel wall its...
Abstract Cancer stem cells (CSC) present a formidable clinical challenge by escaping therapeutic intervention and seeding tumors through processes that remain incompletely understood. Here, we describe small subpopulations of pancreatic cancer with high intrinsic Wnt activity (Wnthigh) possess properties indicative CSCs, including drug resistance tumor-initiating capacity, whereas cell populations negligible (Wntlow) preferentially express markers differentiation. Spontaneous response to...
Abstract Although gemcitabine has been approved as the first-line chemotherapeutic reagent for pancreatic cancer, its response rate is low and average survival duration still only marginal. Because epidermal growth factor receptor (EGFR), vascular endothelial (VEGFR), platelet-derived (PDGFR) modulate tumor progression, we hypothesized that inhibition of phosphorylation all three on cells, tumor-associated stroma cells would improve treatment efficacy in an orthotopic model nude mice prolong...
Experimental metastases in the brain of mice are infiltrated by microglia, and parabiosis experiments green fluorescent protein (GFP(+)) GFP(-) revealed that these microglia derived from circulating monocytes (GFP(+), F4/80(+), CD68(+)). These findings raised question as to whether (specialized macrophages) possess tumoricidal activity. C8-B4 murine cells were incubated vitro medium (control) or containing both lipopolysaccharide interferon-γ. Control not against a number human tumor cells,...
Abstract The extensive phenotypic and functional heterogeneity of cancer cells plays an important role in tumor progression therapeutic resistance. Characterizing this identifying invasive phenotype may provide possibility to improve chemotherapy treatment. By mimicking cell perfusion through circulatory system metastasis, we develop a unique microfluidic cytometry (MC) platform separate at high throughput further derive physical parameter ‘transportability’ characterize the ability pass...
Therapies targeted to the immune system, such as immunotherapy, are currently shaping a new, rapidly developing branch of promising cancer treatments, offering potential change prognosis previously non-responding patients. Macrophages comprise most abundant population cells in tumor microenvironment (TME) and can undergo differentiation into functional phenotypes depending on local tissue environment. Based these phenotypes, associated macrophages (TAM) either aid progression (M2 phenotype)...
The proposed experimental/computational approach could enable prediction of nanotherapeutics performance to treat hypovascularized metastatic cancer in the liver.
Abstract Purpose: We determined whether the administration of tyrosine kinase inhibitor, AEE788, which targets epidermal growth factor receptor and vascular endothelial receptor, alone or in combination with paclitaxel, can inhibit progressive human ovarian carcinoma peritoneal cavity female nude mice. Experimental Design: Western blot analysis immunohistochemical identified optimal dose schedule AEE788 therapy. In several different experiments, paclitaxel-sensitive paclitaxel-resistant...
There is growing evidence implicating that neutrophil gelatinase-associated lipocalin (NGAL) plays a role in the development and progression of cancers. However, effect NGAL colorectal carcinoma (CRC) has not been clearly elucidated. In this study, we investigated tumorigenesis CRC evaluated clinical value expression.We examined expression 526 tissue samples, including 53 sets matched specimens (histologically normal mucosa, adenomas, carcinomas) using immunohistochemical analysis. CRCs,...
Success in anticancer immune therapy relies on stimulation of tumor antigen-specific T lymphocytes and effective infiltration the cells into tissue. Here, a therapeutic vaccine that promotes proliferation both inflamed noninflamed types is described. The consists STING agonist 2'3'-cGAMP, TLR9 ligand CpG, antigen peptides are loaded nanoporous microparticles (
We screened an orthotopic nude mouse model of human pancreatic cancer for candidate serum biomarkers and examined their presence in the plasma patients. Nude mice were injected pancreas with L3.9pl cells. One week later, randomized into 4 treatment groups: i) control, saline; ii) oral STI 571; iii) intraperitoneal gemcitabine; iv) 571 gemcitabine. After 1, 2, 3 weeks treatment, sera tumors collected from each group as well uninjected mice. All analyzed by surface enhanced laser desorption...